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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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9-1
(LD)
,
DE(gpt-lac)5
,
DE(argF-lac)169
,
OCR36,37-6 (LD 9C::Ap)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
betT
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Related strains
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Strains Search
|
Search strains by gene name:
betT
Search strains by all related name:
betT b0314 ECK0312 JW0306
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General information (Go to Linear View: )
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Gene Name
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betT
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Alternative name
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b0314,ECK0312,JW0306
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Location, Length
|
328,687 - 330,720
(
+
)
;
7.08
min
;
2034
(bp) ,
677
(aa)
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Product
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choline transporter of high affinity
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Operon Name
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betT
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Note
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high-affinity choline transport; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006578 - betaine biosynthetic process
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Function
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transport; Transport of small molecules: Other
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Gene Ontology
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GO:0005215
;
transporter activity ( betT )
GO:0005886
;
plasma membrane ( betT )
GO:0006810
;
transport ( betT )
GO:0006950
;
response to stress ( betT )
GO:0016020
;
membrane ( betT )
GO:0016021
;
integral to membrane ( betT )
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PID
|
1786506
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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BETT_ECOLI
(
P17447
)
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- Protein name
|
High-affinity choline transport protein.
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- Synonyms
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- Gene name
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Name=betT; OrderedLocusNames=b0314, z0401, ECs0360;
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- Comments
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FUNCTION:High-affinity uptake of choline driven by a proton-motive force.
PATHWAY:Osmoregulatory choline-glycine betaine pathway.
SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane.
INDUCTION:By osmotic stress. Choline is required for full expression.
SIMILARITY:Belongs to the BCCT transporter (TC 2.A.15) family.
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Entry name(Acc.no)
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Q8FKI6
(
Q8FKI6
)
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- Protein name
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High-affinity choline transport protein.
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- Synonyms
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- Gene name
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Name=betT; OrderedLocusNames=c0434;
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- Comments
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SIMILARITY:Contains 1 HTH luxR-type DNA-binding domain.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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betT
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, E. Coli Pyruvate Dehydrogenase
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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High-affinity choline transport protein
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PSI-BLAST
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High-affinity choline transport protein
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nr
(database updated :
2007.02.20
)
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BLAST
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high-affinity choline transport [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
BCCT
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MMBR References
|
J Gen Microbiol. 1988;134(6):1737-46.
Molecular cloning, physical mapping and expression of the bet genes governing the osmoregulatory choline-glycine betaine pathway of Escherichia coli.
Andresen PA, Kaasen I, Styrvold OB, Boulnois G, Strom AR.
(
3065456
)
|
Mol Microbiol. 1991;5(5):1049-64.
DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli.
Lamark T, Kaasen I, Eshoo MW, Falkenberg P, McDougall J, Strom AR.
(
1956285
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTDLSHSREK DKINPVVFYT SAGLILLFSL TTILFRDFSA LWIGRTLDWV SKTFGWYYLL AATLYIVFVV
0071 CIACSRFGSV KLGPEQSKPE FSLLSWAAML FAAGIGIDLM FFSVAEPVTQ YMQPPEGAGQ TIEAARQAMV
0141 WTLFHYGLTG WSMYALMGMA LGYFSYRYNL PLTIRSALYP IFGKRINGPI GHSVDIAAVI GTIFGIATTL
0211 GIGVVQLNYG LSVLFDIPDS MAAKAALIAL SVIIATISVT SGVDKGIRVL SELNVALALG LILFVLFMGD
0281 TSFLLNALVL NVGDYVNRFM GMTLNSFAFD RPVEWMNNWT LFFWAWWVAW SPFVGLFLAR ISRGRTIRQF
0351 VLGTLIIPFT FTLLWLSVFG NSALYEIIHG GAAFAEEAMV HPERGFYSLL AQYPAFTFSA SVATITGLLF
0421 YVTSADSGAL VLGNFTSQLK DINSDAPGWL RVFWSVAIGL LTLGMLMTNG ISALQNTTVI MGLPFSFVIF
0491 FVMAGLYKSL KVEDYRRESA NRDTAPRPLG LQDRLSWKKR LSRLMNYPGT RYTKQMMETV CYPAMEEVAQ
0561 ELRLRGAYVE LKSLPPEEGQ QLGHLDLLVH MGEEQNFVYQ IWPQQYSVPG FTYRARSGKS TYYRLETFLL
0631 EGSQGNDLMD YSKEQVITDI LDQYERHLNF IHLHREAPGH SVMFPDA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TATCTATTAA AGCGGTTATT GATTGGACGT
-070 TCAATATAAA ATGTGTCTTA ATTGTTACGA ATTTGATTTT AAATAGTAAC AATAACAGTG GGGATACTGG
0001 atgacagacc tttcacacag cagggaaaag gacaaaatca atccggtggt gttttacacc tccgccggac
0071 tgattttgtt gttttccctg acaacgatcc tgtttcgcga cttctcggcc ctgtggattg gccgcacgct
0141 ggactgggtt tctaaaacct tcggttggta ctatctgctg gcggcaacgc tctatattgt ctttgtggtc
0211 tgtatcgctt gttcgcgttt tggttcggtg aagctcgggc cagaacaatc caaaccggaa ttcagcctgc
0281 tgagttgggc ggcgatgctg tttgctgccg ggatcggtat cgacctgatg ttcttctccg tagccgaacc
0351 ggtaacgcag tatatgcagc cgccggaagg cgcgggacag acgattgagg ccgcgcgtca ggcgatggtc
0421 tggacgctgt ttcactacgg cttaaccggc tggtcgatgt atgcgctgat gggcatggcg ctcggatact
0491 ttagctatcg ttataatttg ccgctcacca tccgctcggc gctgtacccg atcttcggta aacggattaa
0561 cgggccgata ggtcactcag tggatattgc agcggtgatc ggcactatct tcggtattgc cactacgctc
0631 ggtatcggtg tggtgcagct taactatggc ttgagcgtac tgtttgatat tcccgattcg atggcggcga
0701 aagcggcact gatcgccttg tcggtgataa tcgccacgat ctctgtcacc tccggtgtcg ataagggcat
0771 tcgcgtgtta tcggagctta atgtcgcgct ggcgctggga ttgatcctgt tcgtattgtt tatgggcgac
0841 acttcgttcc tgcttaatgc actggtgctg aatgttggcg actatgtgaa tcgctttatg ggcatgacgc
0911 tcaacagttt tgccttcgac cgtccggttg agtggatgaa taactggacg ctcttcttct gggcatggtg
0981 ggtggcatgg tcgccgtttg tcggcttgtt cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc
1051 gtgctgggca cgttgattat tccgtttacc ttcacgctgt tatggctctc ggtgttcggc aatagcgcgc
1121 tgtatgaaat catccacggc ggcgcggcat ttgccgagga agcgatggtc catccggagc gcggcttcta
1191 cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca ccattactgg cctgctgttt
1261 tatgtgacct cggcggactc cggggcgctg gtgctgggga atttcacctc gcagcttaaa gatatcaaca
1331 gcgacgcccc cggctggctg cgcgtcttct ggtcggtggc gattggcctg ctgacgctcg gcatgctgat
1401 gactaacggg atatccgcgc tgcaaaacac cacggtgatt atggggctgc cgttcagctt tgtgatcttc
1471 ttcgtgatgg cggggttgta taaatctctg aaggtagaag attaccgccg tgaaagtgcc aaccgcgata
1541 ccgcaccgcg accgctgggg cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc tgatgaatta
1611 tccgggcacg cgttacacta aacagatgat ggagacggtc tgttacccgg caatggaaga agtggcgcag
1681 gagttgcggt tgcgcggcgc gtacgtggag ctaaaaagcc tgccaccgga agagggacag cagttgggtc
1751 atctggattt gttggtgcat atgggcgaag agcaaaactt tgtctatcag atttggccgc agcaatattc
1821 ggtgccgggc tttacctacc gcgcacgcag cggtaaatcg acctactacc ggctggaaac cttcctgtta
1891 gaaggcagcc agggcaacga cctgatggac tacagcaaag agcaggtgat caccgatatt cttgaccagt
1961 acgagcggca ccttaacttt attcatctcc atcgtgaagc gccgggccat agcgtgatgt tcccggacgc
2031 gtgaTTGTTA TTGCATAAAA CCGCGCCATG TCTGCATATG GCGCTGTTTC TCCTACCTCT TGATACGTTA
2101 TATCTATACG GTTAAGCCCT TAGTATCTAT TGAT
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