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PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
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References (PMID)
|
Mol Microbiol. 1996;21(6):1207-18.
RbfA, a 30S ribosomal binding factor, is a cold-shock protein whose absence triggers the cold-shock response.
Jones PG, Inouye M.
(
8898389
)
|
|
|
Deletion
|
OCL29,30-6 (AK4-11 / BK5-11)
(D)
|
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Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
rbfA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
rbfA
Search strains by all related name:
rbfA b3167 ECK3156 f133 JW3136 sdr-43 yhbB
|
General information (Go to Linear View: )
|
|
Gene Name
|
rbfA
|
|
Alternative name
|
b3167,ECK3156,f133,JW3136,sdr-43,yhbB
|
|
Location, Length
|
3,310,799 - 3,311,200
(
-
)
;
71.36
min
;
402
(bp) ,
133
(aa)
|
|
Product
|
30s ribosome binding factor
|
|
Operon Name
|
metY-rimP-nusA-infB-rbfA-truB-rpsO-pnp
|
|
Note
|
ribosome-binding factor A; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009451 - RNA modification
|
|
Function
|
factor; Proteins - translation and modification
|
|
Gene Ontology
|
GO:0005515
;
protein binding ( rbfA )
GO:0005737
;
cytoplasm ( rbfA )
GO:0006364
;
rRNA processing ( rbfA )
|
|
PID
|
1789558
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
rbfA
(
EG11178
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
RBFA_ECOLI
(
P09170
)
|
|
- Protein name
|
Ribosome-binding factor A
|
|
- Synonyms
|
P15B protein
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|
- Gene name
|
OrderedLocusNames=b3167, c3923, z4528, ECs4048, SF3208, S3425;
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- Comments
|
FUNCTION:Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes.
SUBCELLULAR LOCATION:Cytoplasmic (Potential).
SIMILARITY:Belongs to the rbfA family.
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Homology Analysis
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BLAST
|
|
Bacteria
|
|
GTOP
|
rbfA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Ribosome Binding Factor A
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Ribosome-binding factor A (Protein P15B)
|
|
PSI-BLAST
|
Ribosome-binding factor A (Protein P15B)
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
ribosome-binding factor A [Escherichia coli CFT073]
|
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Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
CK2_PHOSPHO_SITE
MYRISTYL
RBFA
|
|
MMBR References
|
Mol Microbiol. 1996;21(6):1207-18.
RbfA, a 30S ribosomal binding factor, is a cold-shock protein whose absence triggers the cold-shock response.
Jones PG, Inouye M.
(
8898389
)
|
J Bacteriol. 1998;180:73-82
RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli.
Bylund, G. O., L. C. Wipemo, L. A. Lundberg, and P. M. Wikstrom.
|
Genes Dev. 1995;9:626-637
Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA.
Dammel, C. S., and H. F. Noller.
|
| Sequences |
Amino acid
FASTA format
|
0001 MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK DEDAVKAGIK
0071 ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMRMS NLVTSVVKHD EERRVNPDDS KED
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gacgctggcg cgtcttatca ggtcaacgtg
-070 aacagccctt ccacctgaaa ttaattttaa aaaggggcta acagcccctt ttttgtcagg agaatttatt
0001 atggcgaaag aatttggtcg cccgcagcgc gtagcgcagg aaatgcaaaa agagatcgct ctcatcctgc
0071 agcgtgaaat taaagatcct cgcctgggca tgatgaccac cgtttccggt gtcgaaatgt ctcgcgacct
0141 ggcgtatgcc aaagtatatg tgacgttcct caacgacaaa gatgaagacg cggttaaagc gggcatcaaa
0211 gcgttgcaag aagcttctgg tttcatccgc agcctgctgg ggaaagcgat gcgcctgcgt atcgtgccgg
0281 aactgacctt cttctacgac aactctctgg ttgaagggat gcgcatgtca aacctggtga ccagcgtggt
0351 caaacatgac gaagaacgtc gtgttaaccc ggacgacagc aaggaggact aatgagtcgt cctcgtcgtc
0421 gcggtcgcga cattaacggc gttttgttgc tggataaacc tcagggtatg tccagcaacg atgcgctgca
0491 aaaagtgaaa cg
|
