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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1991;173(19):5999-6008.
The Escherichia coli htrP gene product is essential for bacterial growth at high temperatures: mapping, cloning, sequencing, and transcriptional regulation of htrP.
Raina S, Mabey L, Georgopoulos C.
(
1917833
)
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Deletion
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OCL36(PC3,OCL35-1)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
ribB
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Related strains
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Strains Search
|
Search strains by gene name:
ribB
Search strains by all related name:
ribB b3041 ECK3032 htrP JW3009 luxH
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General information (Go to Linear View: )
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Gene Name
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ribB
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Alternative name
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b3041,ECK3032,htrP,JW3009,luxH
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Location, Length
|
3,181,835 - 3,182,488
(
-
)
;
68.58
min
;
654
(bp) ,
217
(aa)
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Product
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3,4-dihydroxy-2-butanone-4-phosphate synthase
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Operon Name
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sroG-ribB
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Note
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3,4 dihydroxy-2-butanone-4-phosphate synthase
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Function
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enzyme; Biosynthesis of cofactors, carriers: Riboflavin
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Gene Ontology
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GO:0000287
;
magnesium ion binding ( ribB )
GO:0005515
;
protein binding ( ribB )
GO:0005886
;
plasma membrane ( ribB )
GO:0006950
;
response to stress ( ribB )
GO:0008686
;
3,4-dihydroxy-2-butanone-4-phosphate synthase activity ( ribB )
GO:0009231
;
riboflavin biosynthetic process ( ribB )
GO:0016020
;
membrane ( ribB )
GO:0016829
;
lyase activity ( ribB )
GO:0030145
;
manganese ion binding ( ribB )
GO:0046872
;
metal ion binding ( ribB )
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PID
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1789420
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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ribB
(
EG10465
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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RIBB_ECOLI
(
P24199
)
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- Protein name
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3,4-dihydroxy-2-butanone 4-phosphate synthase
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- Synonyms
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DHBP synthase
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- Gene name
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OrderedLocusNames=b3041, z4399, ECs3929, SF3081, S3286;
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- Comments
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PATHWAY:Riboflavin biosynthesis.
SUBUNIT:Homodimer.
SUBCELLULAR LOCATION:Membrane-associated (Potential).
INDUCTION:Is expressed at all temperatures, but accumulation of transcripts decline with raising temperature. Thus, its expression is repressed by heat shock.
MASS SPECTROMETRY:MW=23352; MW_ERR=2; METHOD=Electrospray; RANGE=1-217; NOTE=Ref.1.
SIMILARITY:Belongs to the DHBP synthase family.
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Entry name(Acc.no)
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RIBB_ECOL6
(
Q8FDH9
)
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- Protein name
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3,4-dihydroxy-2-butanone 4-phosphate synthase
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- Synonyms
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DHBP synthase
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- Gene name
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Name=ribB; OrderedLocusNames=c3795;
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- Comments
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PATHWAY:Riboflavin biosynthesis.
SIMILARITY:Belongs to the DHBP synthase family.
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MMBR References
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Genetika. 1983;19(9):1419-25.
[3 linkage groups of the genes of riboflavin biosynthesis in Escherichia coli]
Bandrin SV, Rabinovich PM, Stepanov AI.
(
6315532
)
|
Sov. Genet. (Engl. Transl. Genetika) 1984;19:1103-1109.
Three linkage groups of genes for riboflavin biosynthesis.
Bandrin, S. V., P. M. Rabinovich, and A. I. Stepanov.
|
J Bacteriol. 1991;173(19):5999-6008.
The Escherichia coli htrP gene product is essential for bacterial growth at high temperatures: mapping, cloning, sequencing, and transcriptional regulation of htrP.
Raina S, Mabey L, Georgopoulos C.
(
1917833
)
|
J Bacteriol. 1992;174:4050-4056
Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli.
Richter, G., R. Volk, C. Krieger, H.-W. Lahm, U. Rothlisberger, and A. Bacher.
|
Cytol. Genet. 1983;17:57-59.
Localization of genes coding cyclohexrolase [cyclohydrolase] II and riboflavin synthase on the Escherichia coli K-12 chromosome.
Teslyar, G. E., and G. M. Shavlovskii.
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| Sequences |
Amino acid
FASTA format
|
0001 MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM TVEQMALTIR HGSGIVCLCI
0071 TEDRRKQLDL PMMVENNTSA YGTGFTVTIE AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL
0141 RAQAGGVLTR GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV TIEDLVAYRQ
0211 AHERKAS
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ctgtcgggca tggacccgct cacgttattt
-070 tggctatatg ccgccactcc taagactgcc ctgattctgg taaccataat tttagtgagg tttttttacc
0001 atgaatcaga cgctactttc ctcttttggt acgcctttcg aacgtgttga aaatgcactg gctgcgctgc
0071 gtgaaggacg cggtgtaatg gtgcttgatg atgaagaccg tgaaaacgaa ggtgatatga tcttcccggc
0141 agaaaccatg actgttgagc agatggcgct gaccattcgc cacggtagcg gtattgtttg cctgtgcatt
0211 actgaagatc gccgtaaaca actcgatctg ccaatgatgg tagaaaataa caccagcgcc tatggcaccg
0281 gttttaccgt gaccattgaa gcagctgaag gtgtgactac cggtgtttct gccgctgacc gtattacgac
0351 cgttcgcgca gcgattgccg atggcgcaaa accgtcagat ctgaatcgtc ctggccacgt tttcccactt
0421 cgcgctcagg caggtggtgt actgacgcgt ggcggtcata ctgaagcaac tattgatctg atgacgctgg
0491 caggctttaa accggctggt gtactgtgtg agctgactaa tgacgatggc acgatggcgc gtgcaccaga
0561 gtgtattgag tttgccaata aacacaatat ggcgctcgtg actattgaag acctggtggc ataccgtcag
0631 gcacatgagc gtaaagccag ctgaaaaccg ctgcttaatt tactgcctta atcaagaaac cgaagttgta
0701 gcaggcttcg gtttttattt ttccctgcta tgcaagatgt taattccgct tctc
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