MG1655
W3110
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Gene Report : metC
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL36 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  metC
Related strains
     Strains Search Search strains by gene name:  metC   Search strains by all related name:  metC b3008 ECK3000 JW2975

General information  (Go to Linear View:)
 Gene Name metC  
 Alternative name b3008,ECK3000,JW2975  
 Location, Length 3,150,258 - 3,151,445 (  +  ) ;   67.90 min ; 1188 (bp) ,   395 (aa) Go to Linear View
 Product cystathionine beta-lyase, PLP-dependent  
 Operon Name metC  
 Note cystathionine beta-lyase (beta-cystathionase); GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009086 - methionine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Methionine  
 Gene Ontology GO:0003824 ; catalytic activity ( metC )
GO:0004121 ; cystathionine beta-lyase activity ( metC )
GO:0005737 ; cytoplasm ( metC )
GO:0006520 ; amino acid metabolic process ( metC )
GO:0008652 ; amino acid biosynthetic process ( metC )
GO:0009086 ; methionine biosynthetic process ( metC )
GO:0016829 ; lyase activity ( metC )
GO:0030170 ; pyridoxal phosphate binding ( metC )
 PID 1789383  
 EC number
  (KEGG Pathway)   		4.4.1.8  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene metC ( EG10583 )  
    Number of removed 4 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) METC_ECOLI ( P06721 )
    -  Protein name Cystathionine beta-lyase  
    -  Synonyms EC 4.4.1.8, CBL, Beta-cystathionase, Cysteine lyase  
    -  Gene name Name=metC; OrderedLocusNames=b3008;  
    -  Comments
  • CATALYTIC ACTIVITY:Cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate.
  • COFACTOR:Pyridoxal phosphate.
  • PATHWAY:Methionine biosynthesis; third step.
  • SUBUNIT:Homotetramer.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Belongs to the trans-sulfuration enzymes family.  
    •   botton Entry name(Acc.no) Q8FDL3 ( Q8FDL3 )
        -  Protein name Cystathionine beta-lyase  
        -  Synonyms EC 4.4.1.8  
        -  Gene name Name=metC; OrderedLocusNames=c3742;  
        -  Comments
  • SIMILARITY:Contains 1 HTH araC/xylS-type DNA-binding domain.  
    •   botton Entry name(Acc.no) Q8XBT9 ( Q8XBT9 )
        -  Protein name Cystathionine beta-lyase  
        -  Synonyms Beta-cystathionase  
        -  Gene name Name=metC; OrderedLocusNames=z4361;  
        -  Comments
  • SIMILARITY:Contains 1 HTH araC/xylS-type DNA-binding domain.  

    •  Linear View (Whole Mode)
    View Location
       3140.0  –  3165.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP metC (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Methionine Gamma-Lyase (Mgl) From Trichomonas Vaginalis In Complex With Propargylglycine  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Cystathionine beta-lyase  
             PSI-BLAST Serine hydroxymethyltransferase
        nr     (database updated : 2007.02.20 )
             BLAST Chain A, Cystathionine Beta-Lyase (Cbl) From Escherichia Coli In Complex With N-Hydrazinocarbonylmethyl-2-Trifluoromethyl- Benzamide  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    CYS_MET_METAB_PP     

    Other Cross-Reference
        COG COG0626E 
        EcoCyc metC 

    MMBR References
    Proc Natl Acad Sci U S A. 1986;83:867-871
    Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region.
    Belfaiza, J. C., C. Parsot, A. Martel, C. Bouthier de la Tour, D. Margarita, G. N. Cohen, and I. Saint-Girons.
    FEMS Microbiol Lett. 1984;22:31-35.
    The metC gene in Escherichia coli K-12: isolation and studies of relatedness in Enterobacteriaceae.
    Michaeli, S., and E. Z. Ron.

    Sequences
    Amino acid
    FASTA format 
    0001 MADKKLDTQL VNAGRSKKYT LGAVNSVIQR ASSLVFDSVE AKKHATRNRA NGELFYGRRG TLTHFSLQQA 
0071 MCELEGGAGC VLFPCGAAAV ANSILAFIEQ GDHVLMTNTA YEPSQDFCSK ILSKLGVTTS WFDPLIGADI 
0141 VKHLQPNTKI VFLESPGSIT MEVHDVPAIV AAVRSVVPDA IIMIDNTWAA GVLFKALDFG IDVSIQAATK 
0211 YLVGHSDAMI GTAVCNARCW EQLRENAYLM GQMVDADTAY ITSRGLRTLG VRLRQHHESS LKVAEWLAEH 
0281 PQVARVNHPA LPGSKGHEFW KRDFTGSSGL FSFVLKKKLN NEELANYLDN FSLFSMAYSW GGYESLILAN 
0351 QPEHIAAIRP QGEIDFSGTL IRLHIGLEDV DDLIADLDAG FARIV
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             ATTTGCGAAA ATTTTCTGCT TTATGCCAAT 
-070 TCTTCAGGAT GCGCCCGCGA ATATTCATGC TAGTTTAGAC ATCCAGACGT ATAAAAACAG GAATCCCGAC 
0001 atggcggaca aaaagcttga tactcaactg gtgaatgcag gacgcagcaa aaaatacact ctcggcgcgg 
0071 taaatagcgt gattcagcgc gcttcttcgc tggtctttga cagtgtagaa gccaaaaaac acgcgacacg 
0141 taatcgcgcc aatggagagt tgttctatgg acggcgcgga acgttaaccc atttctcctt acaacaagcg 
0211 atgtgtgaac tggaaggtgg cgcaggctgc gtgctatttc cctgcggggc ggcagcggtt gctaattcca 
0281 ttcttgcttt tatcgaacag ggcgatcatg tgttgatgac caacaccgcc tatgaaccga gtcaggattt 
0351 ctgtagcaaa atcctcagca aactgggcgt aacgacatca tggtttgatc cgctgattgg tgccgatatc 
0421 gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg aatcgccagg ctccatcacc atggaagtcc 
0491 acgacgttcc ggcgattgtt gccgccgtac gcagtgtggt gccggatgcc atcattatga tcgacaacac 
0561 ctgggcagcc ggtgtgctgt ttaaggcgct ggattttggc atcgatgttt ctattcaagc cgccaccaaa 
0631 tatctggttg ggcattcaga tgcgatgatt ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac 
0701 gggaaaatgc ctatctgatg ggccagatgg tcgatgccga taccgcctat ataaccagcc gtggcctgcg 
0771 cacattaggt gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg ctgaatggct ggcagaacat 
0841 ccgcaagttg cgcgagttaa ccaccctgct ctgcctggca gtaaaggtca cgaattctgg aaacgagact 
0911 ttacaggcag cagcgggcta ttttcctttg tgcttaagaa aaaactcaat aatgaagagc tggcgaacta 
0981 tctggataac ttcagtttat tcagcatggc ctactcgtgg ggcgggtatg aatcgttgat cctggcaaat 
1051 caaccagaac atatcgccgc cattcgccca caaggcgaga tcgattttag cgggaccttg attcgcctgc 
1121 atattggtct ggaagatgtc gacgatctga ttgccgatct ggacgccggt tttgcgcgaa ttgtataaCA 
1191 TTGCCACTTT TGGACAATTT TGCAGACATT TTATTGTGAA AAGTCTTAAA TTGTTGCGTC CGGGATCAAG 
1261 GCGTCCCGGA CGATTCAGGA GTACAATA