MG1655
W3110
Search for
Japanese | English
Top Genes Deletions Circular Linear Motif Contigs Reference SequenceCutter Download Strains TEC
Gene Report : prfB
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1988;170(11):5378-81.
Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli.
Kawakami K, Inada T, Nakamura Y. ( 3053663 )
     Deletion OCL41,42-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  prfB
Related strains
     Strains Search Search strains by gene name:  prfB   Search strains by all related name:  prfB b2891 ECK2886 JW5847 supK

General information  (Go to Linear View:)
 Gene Name prfB  
 Alternative name b2891,ECK2886,JW5847,supK  
 Location, Length 3,033,206 - 3,034,304 (  -  ) ;   65.38 min ; 1099 (bp) ,   365 (aa) Go to Linear View
 Product peptide chain release factor RF-2  
 Operon Name xerD-dsbC-recJ-prfB-lysS  
 Note an in-frame premature UGA termination codon is located within the prfB sequence, and a naturally occuring +1 frameshift is required for synthesis of RF-2; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation  
 Function factor; Proteins - translation and modification,2.3.2 information transfer; protein related; translation,7.1 location of gene products; cytoplasm  
 Gene Ontology GO:0003747 ; translation release factor activity ( prfB )
GO:0005737 ; cytoplasm ( prfB )
GO:0006412 ; translation ( prfB )
GO:0006415 ; translational termination ( prfB )
GO:0016149 ; translation release factor activity, codon specific ( prfB )
 PID 2367172  
 EC number
  (KEGG Pathway)   		 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene prfB ( EG10762 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) RF2_ECOLI ( P07012 ; P76642 )
    -  Protein name Peptide chain release factor 2  
    -  Synonyms RF-2  
    -  Gene name Name=prfB; Synonyms=supK; OrderedLocusNames=b2891;  
    -  Comments
  • FUNCTION:Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • PTM:Methylated by hemK. Methylation increases the termination efficiency of RF2. Is absent when the factor is overproduced.
  • MISCELLANEOUS:The gene for this protein contains a UGA in-frame termination codon after Leu-25; a naturally occurring frameshift enables complete translation of RF-2. This provides a mechanism for the protein to regulate its own production.
  • SIMILARITY:Belongs to the prokaryotic/mitochondrial release factor family.  
    •   botton Entry name(Acc.no) RF2_ECOL6 ( Q8XD56 )
        -  Protein name Peptide chain release factor 2  
        -  Synonyms RF-2  
        -  Gene name Name=prfB; OrderedLocusNames=c5622, z4229, ECs3763, SF2877, S3076;  
        -  Comments
  • FUNCTION:Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (By similarity).
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • PTM:Methylated by hemK. Methylation increases the termination efficiency of RF2. Is absent when the factor is overproduced (By similarity).
  • MISCELLANEOUS:The gene for this protein contains a UGA in-frame termination codon after Leu-25; a naturally occurring frameshift enables complete translation of RF-2. This provides a mechanism for the protein to regulate its own production (By similarity).
  • SIMILARITY:Belongs to the prokaryotic/mitochondrial release factor family.  

    •  Linear View (Whole Mode)
    View Location
       3020.0  –  3045.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP prfB (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Docking Of The Modified Rf2 X-Ray Structure Into The Low Resolution Cryo-Em Map Of Rf2 E.Coli 70s Ribosome  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Peptide chain release factor 2  
             PSI-BLAST Peptide chain release factor 1
        nr     (database updated : 2007.02.20 )
             BLAST peptide chain release factor 2 [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    LEUCINE_ZIPPER    RF_PROK_I     

    Other Cross-Reference
        COG COG1186J 
        EcoCyc prfB 

    MMBR References
    J Biol Chem. 1989;264:20054-20059
    Molecular cloning and expression of ribosome releasing factor.
    Ichikawa, S., and A. Kaji.
    Proc Natl Acad Sci U S A. 1988;85:5620-5624
    Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli.
    Kawakami, K., Y. H. Jonsson, G. R. Bjork, H. Ikeda, and H. Nakamura.
    J Bacteriol. 1995;177:5547-5553
    Comparative characterization of release factor RF-3 genes of Escherichia coli, Salmonella typhimurium, and Dichelobacter nodosus.
    Kawazu, Y., K. Ito, K. Matsumura, and Y. Nakamura.
    J Bacteriol. 1988;170(10):4537-41.
    Rapid and precise mapping of the Escherichia coli release factor genes by two physical approaches.
    Lee CC, Kohara Y, Akiyama K, Smith CL, Craigen WJ, Caskey CT. ( 3049538 )

    Sequences
    Amino acid
    FASTA format 
    0001 MFEINPVNNR IQDLTERSDV LRGYLDYDAK KERLEEVNAE LEQPDVWNEP ERAQALGKER SSLEAVVDTL 
0071 DQMKQGLEDV SGLLELAVEA DDEETFNEAV AELDALEEKL AQLEFRRMFS GEYDSADCYL DIQAGSGGTE 
0141 AQDWASMLER MYLRWAESRG FKTEIIEESE GEVAGIKSVT IKISGDYAYG WLRTETGVHR LVRKSPFDSG 
0211 GRRHTSFSSA FVYPEVDDDI DIEINPADLR IDVYRTSGAG GQHVNRTESA VRITHIPTGI VTQCQNDRSQ 
0281 HKNKDQAMKQ MKAKLYELEM QKKNAEKQAM EDNKSDIGWG SQIRSYVLDD SRIKDLRTGV ETRNTQAVLD 
0351 GSLDQFIEAS LKAGL
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             gccaatttag cgtcatcttc tctataaaaa 
-070 agagcgtgga ttgggtacaa tcccgctctt atcaccgcat tttgactagc tcaataaaag aaatcagacc 
0001 atgtttgaaa ttaatccggt aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt 
0071 atctttgact acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct 
0141 ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct 
0211 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc tgacgacgaa 
0281 gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact ggcgcagctt gagttccgcc 
0351 gtatgttctc tggcgaatat gacagcgccg actgctacct cgatattcag gcggggtctg gcggtacgga 
0421 agcacaggac tgggcgagca tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact 
0491 gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt 
0561 acgcttacgg ctggctgcgt acagaaaccg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg 
0631 cggtcgtcgc cacacgtcgt tcagctccgc gtttgtttat ccggaagttg atgatgatat tgatatcgaa 
0701 atcaacccgg cggatctgcg cattgacgtt tatcgcacgt ccggcgcggg cggtcagcac gttaaccgta 
0771 ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat cgtgacccag tgccagaacg accgttccca 
0841 gcacaagaac aaagatcagg ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa 
0911 aatgccgaga aacaggcgat ggaagataac aaatccgaca tcggctgggg cagccagatt cgttcttatg 
0981 tccttgatga ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga 
1051 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat gtctgaacaa 
1121 cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac gcgtcgtgag aagctggcga 
1191 acctgcgcg