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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(argA-thyA)232
,
OCL44,45-11
(D)
,
OCL44,45-15 (AK1-9 / BK2-9)
(D)
,
OCL44,45-3
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
recD
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Related strains
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Strains Search
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Search strains by gene name:
recD
Search strains by all related name:
recD b2819 ECK2815 hopE JW2787
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General information (Go to Linear View: )
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Gene Name
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recD
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Alternative name
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b2819,ECK2815,hopE,JW2787
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Location, Length
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2,948,657 - 2,950,483
(
-
)
;
63.55
min
;
1827
(bp) ,
608
(aa)
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Product
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exonuclease V (RecBCD complex), alpha chain
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Operon Name
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ptrA-recBD
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Note
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DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease; GO_process: GO:0006308 - DNA catabolic process; GO_process: GO:0006310 - DNA recombination
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Function
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enzyme; Degradation of DNA
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Gene Ontology
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GO:0000166
;
nucleotide binding ( recD )
GO:0004386
;
helicase activity ( recD )
GO:0004518
;
nuclease activity ( recD )
GO:0004519
;
endonuclease activity ( recD )
GO:0004527
;
exonuclease activity ( recD )
GO:0005524
;
ATP binding ( recD )
GO:0006281
;
DNA repair ( recD )
GO:0006974
;
response to DNA damage stimulus ( recD )
GO:0008854
;
exodeoxyribonuclease V activity ( recD )
GO:0009338
;
exodeoxyribonuclease V complex ( recD )
GO:0016787
;
hydrolase activity ( recD )
GO:0017111
;
nucleoside-triphosphatase activity ( recD )
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PID
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1789182
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EC number
(KEGG Pathway)
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3.1.11.5
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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recD
(
EG10826
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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EX5A_ECOLI
(
P04993
;
Q59378
)
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- Protein name
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Exodeoxyribonuclease V alpha chain
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- Synonyms
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EC 3.1.11.5, Exodeoxyribonuclease V 67 kDa polypeptide
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- Gene name
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Name=recD; OrderedLocusNames=b2819;
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- Comments
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FUNCTION:Exhibits several catalytic activities, including ATP-dependent exonuclease, ATP-stimulated endonuclease, ATP-dependent unwinding and DNA-dependent ATPase activities. Strand cleavage occurs 5' to 3' during the unwinding of duplex DNA at CHI sequences, which locally stimulate recombination.
CATALYTIC ACTIVITY:Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.
SUBUNIT:Consist of three subunits; recB, recC and recD.
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Entry name(Acc.no)
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Q8FEB4
(
Q8FEB4
)
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- Protein name
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Exodeoxyribonuclease V alpha chain
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- Synonyms
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EC 3.1.11.5
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- Gene name
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Name=recD; OrderedLocusNames=c3413;
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- Comments
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SIMILARITY:Belongs to the ATP-dependent AMP-binding enzyme family.
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Entry name(Acc.no)
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Q8X6N0
(
Q8X6N0
)
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- Protein name
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DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease.
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- Synonyms
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- Gene name
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Name=recD; OrderedLocusNames=z4136;
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- Comments
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SIMILARITY:Belongs to the beta-ketoacyl-ACP synthases family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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recD
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain D, Recbcd:dna Complex
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Exodeoxyribonuclease V alpha chain
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PSI-BLAST
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UvrABC system protein B
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nr
(database updated :
2007.02.20
)
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BLAST
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exonuclease V (RecBCD complex), alpha chain [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
RGD
ATP_GTP_A
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MMBR References
|
Nucleic Acids Res. 1986;14:8583-8594
Complete nucleotide sequence of recD, the structural gene for the alpha subunit of exonuclease V of Escherichia coli.
Finch, P. W., A. Storey, K. Brown, I. D. Hickson, and P. T. Emmerson.
|
Mol Gen Genet. 1990;224(1):1-9.
Linear multimer formation of plasmid DNA in Escherichia coli hopE (recD) mutants.
Niki H, Ogura T, Hiraga S.
(
2177520
)
|
J Bacteriol. 1986;167(2):594-603.
Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli.
Biek DP, Cohen SN.
(
3015881
)
|
J Biol Chem. 1995;270:24451-24458
Monomeric RecBCD enzyme binds and unwinds DNA.
Taylor, A. F., and G. R. Smith.
|
Biochimie. 1991;73(4):523-32.
rec genes and homologous recombination proteins in Escherichia coli.
Clark AJ.
(
1911953
)
|
Proc Natl Acad Sci U S A. 1986;83:5558-5562
recD: the gene for an essential third subunit of exonuclease V.
Amundsen, S. K., A. F. Taylor, A. M. Chaudhury, and G. R. Smith.
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| Sequences |
Amino acid
FASTA format
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0001 MKLQKQLLEA VEHKQLRPLD VQFALTVAGD EHPAVTLAAA LLSHDAGEGH VCLPLSRLEN NEASHPLLAT
0071 CVSEIGELQN WEECLLASQA VSRGDEPTPM ILCGDRLYLN RMWCNERTVA RFFNEVNHAI EVDEALLAQT
0141 LDKLFPVSDE INWQKVAAAV ALTRRISVIS GGPGTGKTTT VAKLLAALIQ MADGERCRIR LAAPTGKAAA
0211 RLTESLGKAL RQLPLTDEQK KRIPEDASTL HRLLGAQPGS QRLRHHAGNP LHLDVLVVDE ASMIDLPMMS
0281 RLIDALPDHA RVIFLGDRDQ LASVEAGAVL GDICAYANAG FTAERARQLS RLTGTHVPAG TGTEAASLRD
0351 SLCLLQKSYR FGSDSGIGQL AAAINRGDKT AVKTVFQQDF TDIEKRLLQS GEDYIAMLEE ALAGYGRYLD
0421 LLQARAEPDL IIQAFNEYQL LCALREGPFG VAGLNERIEQ FMQQKRKIHR HPHSRWYEGR PVMIARNDSA
0491 LGLFNGDIGI ALDRGQGTRV WFAMPDGNIK SVQPSRLPEH ETTWAMTVHK SQGSEFDHAA LILPSQRTPV
0561 VTRELVYTAV TRARRRLSLY ADERILSAAI ATRTERRSGL AALFSSRE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aagaacatcc gcaacagggg atttacacaa
-070 cccgacccaa cgccgggttg attgccctga tggatgagat gtttgccggt atgaccctgg aggaggcgta
0001 atgaaattgc aaaagcaatt actggaagct gtggagcaca aacagctacg cccgctggat gtgcaatttg
0071 ccctgaccgt ggcgggagat gaacatcctg ccgtcaccct cgcggcggca ctgttaagtc atgatgccgg
0141 agagggacac gtttgtttgc cgctttcacg actggaaaat aacgaggcgt cgcatccgct gttggcgacc
0211 tgtgtcagtg aaatcggtga gctacaaaat tgggaagaat gcttgctggc ttctcaagcg gtcagcaggg
0281 gagatgaacc cacgccgatg atcctctgtg gcgatcgtct ttatttgaat cgcatgtggt gtaacgagcg
0351 cacagtggca cgctttttca acgaagtgaa tcatgccatt gaggttgatg aagctctact ggcgcaaacc
0421 ctggacaaac tttttccagt aagcgatgaa attaactggc aaaaagttgc ggcggcagtg gcgctgacgc
0491 ggcggatctc ggtgatttcc ggcggccctg gcaccggtaa aacgaccacc gtagcgaagt tgctggcagc
0561 gttaattcaa atggccgacg gcgaacgctg ccgtatccgt ctggctgcac caacgggtaa agctgccgcg
0631 cgcttaaccg aatctctcgg caaggctttg cgacagttac cgctgaccga tgaacaaaag aaacgcattc
0701 cggaagatgc cagcactttg caccgattgc tgggcgcgca gccgggtagc cagcgtttac gtcatcatgc
0771 cggtaacccg ctgcatcttg atgtgctggt ggtagatgaa gcgtcaatga tcgatctgcc tatgatgtcg
0841 agactgatcg acgccttgcc cgatcatgcg cgagtgatct ttctcggcga tcgtgatcaa ctggcctcgg
0911 ttgaggctgg ggctgtgctg ggcgatatct gcgcttatgc caacgcgggc tttaccgccg agcgtgccag
0981 gcagctaagc cgcctgacgg ggactcacgt tccggcagga accggcacag aagcggcatc tttgcgcgac
1051 agtctctgcc tgctgcaaaa aagctatcgt ttcggcagcg attctggcat tggtcagtta gctgcggcga
1121 tcaaccgtgg tgataaaacg gcagtgaaaa ccgtttttca gcaggatttt actgatatcg aaaaacggct
1191 tttacagagc ggcgaagatt atattgcgat gcttgaggaa gctcttgcgg gttacggacg ttatctggat
1261 ctgctgcaag cgcgtgccga gccggattta atcattcagg cgttcaatga gtaccagctt ttgtgcgccc
1331 tgcgggaagg gccgtttggc gtggctggac tgaatgagcg aattgagcag tttatgcaac agaagcgcaa
1401 aattcatcgt catccgcact ctcgttggta cgaaggtcga ccggtgatga ttgcccgtaa tgacagcgcg
1471 cttgggttgt ttaatggcga tatcggtatt gcgctggatc gcgggcaggg gacgcgcgtc tggtttgcga
1541 tgccggacgg caatattaag tctgtgcaac cgagtcgctt gccagagcac gaaactacgt gggcgatgac
1611 ggtacataaa tcgcagggat cggagttcga ccatgcggcg ttgattttgc cgagccaacg cacgccggta
1681 gtaacgcgag agctggttta taccgcggtg acccgcgcgc gtcgccgtct gtcgctgtat gccgatgagc
1751 gcatattaag tgcggcaatc gccactcgta ctgagcggcg cagtggtctg gcggcgttgt ttagttcacg
1821 ggaataaacg taattgccgg atgcgacgta cgagtgttac gcatgtcgca tccgacgatt ttcatcgctt
1891 accctaaatc cgccatcaac actttggatt tacgctg
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