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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCL46
(D)
,
OCL46-2
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
cysD
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Related strains
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Strains Search
|
Search strains by gene name:
cysD
Search strains by all related name:
cysD b2752 ECK2747 JW2722
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General information (Go to Linear View: )
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Gene Name
|
cysD
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Alternative name
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b2752,ECK2747,JW2722
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Location, Length
|
2,873,443 - 2,874,351
(
-
)
;
61.93
min
;
909
(bp) ,
302
(aa)
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|
Product
|
sulfate adenylyltransferase, subunit 2
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Operon Name
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cysDNC
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Note
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ATP:sulfurylase (ATP:sulfate adenylyltransferase), subunit 2; GO_process: GO:0006790 - sulfur metabolic process
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Function
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enzyme; Central intermediary metabolism: Sulfur metabolism
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Gene Ontology
|
GO:0000103
;
sulfate assimilation ( cysD )
GO:0004781
;
sulfate adenylyltransferase (ATP) activity ( cysD )
GO:0008152
;
metabolic process ( cysD )
GO:0016740
;
transferase activity ( cysD )
GO:0016779
;
nucleotidyltransferase activity ( cysD )
GO:0019421
;
sulfate reduction, APS pathway ( cysD )
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|
PID
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1789109
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EC number
(KEGG Pathway)
|
2.7.7.4
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
cysD
(
EG10186
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
|
CYSD_ECOLI
(
P21156
)
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- Protein name
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Sulfate adenylyltransferase subunit 2
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- Synonyms
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EC 2.7.7.4, Sulfate adenylate transferase, SAT, ATP-sulfurylase small subunit
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- Gene name
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Name=cysD; OrderedLocusNames=b2752;
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- Comments
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CATALYTIC ACTIVITY:ATP + sulfate = diphosphate + adenylylsulfate.
ENZYME REGULATION:Stimulated by an intrinsic GTPase (Probably cysN).
PATHWAY:First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway.
SUBUNIT:Heterodimer composed of cysD, the smaller subunit, and cysN.
SIMILARITY:Belongs to the PAPS reductase family. CysD subfamily.
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Entry name(Acc.no)
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CYSD_ECOL6
(
Q8FEJ0
)
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- Protein name
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Sulfate adenylyltransferase subunit 2
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- Synonyms
|
EC 2.7.7.4, Sulfate adenylate transferase, SAT, ATP-sulfurylase small subunit
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- Gene name
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Name=cysD; OrderedLocusNames=c3319;
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- Comments
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CATALYTIC ACTIVITY:ATP + sulfate = diphosphate + adenylylsulfate.
PATHWAY:First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway.
SUBUNIT:Heterodimer composed of cysD, the smaller subunit, and cysN (By similarity).
SIMILARITY:Belongs to the PAPS reductase family. CysD subfamily.
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Entry name(Acc.no)
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CYSD_ECO57
(
Q8X7X4
)
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- Protein name
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Sulfate adenylyltransferase subunit 2
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- Synonyms
|
EC 2.7.7.4, Sulfate adenylate transferase, SAT, ATP-sulfurylase small subunit
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- Gene name
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Name=cysD; OrderedLocusNames=z4060, ECs3606;
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- Comments
|
CATALYTIC ACTIVITY:ATP + sulfate = diphosphate + adenylylsulfate.
PATHWAY:First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway.
SUBUNIT:Heterodimer composed of cysD, the smaller subunit, and cysN (By similarity).
SIMILARITY:Belongs to the PAPS reductase family. CysD subfamily.
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MMBR References
|
J Gen Microbiol. 1987;133(10):2707-17.
Lambda transducing phage and clones carrying genes of the cysJIHDC gene cluster of Escherichia coli K12.
Hunt CL, Colless V, Smith MT, Molasky DO, Malo MS, Loughlin RE.
(
2966849
)
|
Gene. 1990;87(1):127-31.
Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12.
Malo MS, Loughlin RE.
(
2185135
)
|
Biotechnol Appl Biochem. 1990;12(2):212-6.
Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12.
Tei H, Murata K, Kimura A.
(
2158792
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MDQIRLTHLR QLEAESIHII REVAAEFSNP VMLYSIGKDS SVMLHLARKA FYPGTLPFPL LHVDTGWKFR
0071 EMYEFRDRTA KAYGCELLVH KNPEGVAMGI NPFVHGSAKH TDIMKTEGLK QALNKYGFDA AFGGARRDEE
0141 KSRAKERIYS FRDRFHRWDP KNQRPELWHN YNGQINKGES IRVFPLSNWT EQDIWQYIWL ENIDIVPLYL
0211 AAERPVLERD GMLMMIDDNR IDLQPGEVIK KRMVRFRTLG CWPLTGAVES NAQTLPEIIE EMLVSTTSER
0281 QGRVIDRDQA GSMELKKRQG YF
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gaactactca ttctaattgg taatttcatt
-070 cgttctctta cgctccctat agtcgaaaca tctgatggca agaaaatagc ggtattgcaa aggaacggtt
0001 atggatcaaa tacgacttac tcacctgcgg caactggagg cggaaagcat ccacattatt cgcgaggtgg
0071 cggcagaatt ctcaaatccg gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc
0141 gcgcaaggcg ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
0211 gagatgtatg agttccgcga tcgtactgct aaagcctacg gctgcgaact gctggtgcat aaaaacccgg
0281 aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat accgatatta tgaaaactga
0351 aggcctgaaa caggcgctga acaaatacgg ttttgatgcc gccttcggtg gtgcgcgccg tgacgaagag
0421 aaatcccgcg ctaaagagcg aatttactct ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc
0491 gcccggagct gtggcacaac tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc
0561 taactggacc gagcaggata tctggcaata catctggctg gaaaatatcg acattgttcc gctatatctc
0631 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt atcgacctgc
0701 aaccgggcga agtgattaaa aaacggatgg tgcgtttccg tacgctgggc tgctggccgc tgaccggtgc
0771 ggtggagtca aatgcacaaa cactgccgga aatcatcgaa gagatgctgg tttccaccac cagtgaacgt
0841 cagggccgcg tgattgaccg cgaccaggcg gggtctatgg agctgaaaaa acgtcagggg tatttttaag
0911 atgaacaccg cacttgcaca acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac
0981 ataaaagcct gctgcgtttt ctgacctgt
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