MG1655
W3110
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Gene Report : rpoS
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Gen Genet. 1995;248(2):190-4.
Sigma S-dependent overexpression of ftsZ in an Escherichia coli K-12 rpoB mutant that is resistant to the division inhibitors DicB and DicF RNA.
Cam K, Cuzange A, Bouche JP. ( 7651342 )
     Deletion OCL46 (D)  ,  OCL46-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rpoS
Related strains
     Strains Search Search strains by gene name:  rpoS   Search strains by all related name:  rpoS abrD appR b2741 csi2 dpeB ECK2736 JW5437 katF nur otsX sigS

General information  (Go to Linear View:)
 Gene Name rpoS  
 Alternative name abrD,appR,b2741,csi2,dpeB,ECK2736,JW5437,katF,nur,otsX,sigS  
 Location, Length 2,864,581 - 2,865,573 (  -  ) ;   61.74 min ; 993 (bp) ,   330 (aa) Go to Linear View
 Product RNA polymerase, sigma S (sigma 38) factor  
 Operon Name nlpD-rpoS  
 Note RNA polymerase, sigma S (sigma38) factor; synthesis of many growth phase related proteins; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006350 - transcription; GO_process: GO:0006970 - response to osmotic stress  
 Function enzyme; Global regulatory functions  
 Gene Ontology GO:0003677 ; DNA binding ( rpoS )
GO:0003700 ; transcription factor activity ( rpoS )
GO:0006350 ; transcription ( rpoS )
GO:0006352 ; transcription initiation ( rpoS )
GO:0006355 ; regulation of transcription, DNA-dependent ( rpoS )
GO:0016987 ; sigma factor activity ( rpoS )
 PID 1789098  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) RPOS_ECOLI ( P13445 ; Q79EB7 )
    -  Protein name RNA polymerase sigma factor rpoS  
    -  Synonyms Sigma-38  
    -  Gene name OrderedLocusNames=b2741;  
    -  Comments
  • FUNCTION:Sigma transcription factor controlling a regulon of genes required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon and other genes by modifying the affinity of RNA polymerase for the promoters.
  • SIMILARITY:Belongs to the sigma-70 factor family.  
    •   botton Entry name(Acc.no) Q8XEE1 ( Q8XEE1 )
        -  Protein name RNA polymerase, sigma S (Sigma38) factor; synthesis of many growth phase related proteins.  
        -  Synonyms  
        -  Gene name Name=rpoS; OrderedLocusNames=z4049;  
        -  Comments
  • FUNCTION:Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity).
  • SIMILARITY:Belongs to the sigma-70 factor family.  

    •  Linear View (Whole Mode)
    View Location
       2855.0  –  2880.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP rpoS (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain F, Crystal Structure Of The Rna Polymerase Holoenzyme From Thermus Thermophilus At 2.6a Resolution  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST RNA polymerase sigma factor rpoS  
             PSI-BLAST RNA polymerase sigma factor rpoD
        nr     (database updated : 2007.02.20 )
             BLAST RNA polymerase sigma factor [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    RGD    SIGMA70_1    SIGMA70_2     

    Other Cross-Reference
        COG COG0568K 
        EcoCyc rpoS 

    MMBR References
    J Bacteriol. 1998;180:1030-1036
    Importance of RpoS and Dps in survival of exposure of both exponential- and stationary-phase Escherichia coli cells to the electrophile N-ethylmaleimide.
    Ferguson, G. P., R. I. Creighton, Y. Nikolaev, and I. R. Booth.
    J Bacteriol. 1993;175:7982-7989
    Synthesis of the stationary-phase sigma factor s is positively regulated by ppGpp.
    Gentry, D. R., V. J. Hernandez, L. H. Nguyen, D. B. Jensen, and M. Cashel.
    J Bacteriol. 1993;175(9):2483-9.
    Protein-protein communication within the transcription apparatus.
    Ishihama A. ( 8478317 )
    J Bacteriol. 1998;180:1095-1102
    Multiple regions on the Escherichia coli heat shock transcription factor sigma32 determine core RNA polymerase binding specificity.
    Joo, D. M., A. Nolte, R. Calendar, Y. N. Zhou, and D. J. Jin.
    J Bacteriol. 1992;174:889-898
    Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR).
    Kaasen, I., P. Falkenberg, O. B. Styrvold, and A. R. Strom.
    J Bacteriol. 1995;177:4676-4680
    Identification of transcriptional start sites and the role of ppGpp in the expression of rpoS, the structural gene for the sigma S subunit of RNA polymerase in Escherichia coli.
    Lange, R., D. Fischer, and R. Hengge-Aronis.
    Mol Microbiol. 1991;5(1):49-59.
    Identification of a central regulator of stationary-phase gene expression in Escherichia coli.
    Lange R, Hengge-Aronis R. ( 1849609 )
    J Bacteriol. 1984;160(2):668-75.
    Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli.
    Loewen PC, Triggs BL. ( 6094482 )
    J Bacteriol. 1994;176:5378-5384
    Evidence for involvement of proteins HU and RpoS in transcription of the osmoresponsive proU operon in Escherichia coli.
    Manna, D., and J. Gowrishankar.
    Gene. 1988;73(2):337-45.
    Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli.
    Mulvey MR, Sorby PA, Triggs-Raine BL, Loewen PC. ( 2977357 )
    Nucleic Acids Res. 1989;17(23):9979-91.
    Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor.
    Mulvey MR, Loewen PC. ( 2690013 )
    J Bacteriol. 1998;180:846-854
    Roles of DnaK and RpoS in starvation-induced thermotolerance of Escherichia coli.
    Rockabrand, D., K. Livers, T. Austin, R. Kaiser, D. Jensen, R. Burgess, and P. Blum.
    Proc Natl Acad Sci U S A. 1989;86:3271-3275
    Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF gene product.
    Sak, B. D., A. Eisenstark, and D. Touati.
    J Bacteriol. 1986;168(1):13-21.
    Control of sensitivity to inactivation by H2O2 and broad-spectrum near-UV radiation by the Escherichia coli katF locus.
    Sammartano LJ, Tuveson RW, Davenport R. ( 3019996 )
    J Bacteriol. 1992;174:4769-4776
    Regulation of katF and katE in Escherichia coli K-12 by weak acids.
    Schellhorn, H. E., and V. L. Stones.
    Res Microbiol. 1991;142(1):29-36.
    Are appR and katF the same Escherichia coli gene encoding a new sigma transcription initiation factor?
    Touati E, Dassa E, Dassa J, Boquet PL, Touati D. ( 1648776 )
    Mol Gen Genet. 1986;202(2):257-64.
    Pleiotropic mutations in appR reduce pH 2.5 acid phosphatase expression and restore succinate utilisation in CRP-deficient strains of Escherichia coli.
    Touati E, Dassa E, Boquet PL. ( 3517593 )
    J Bacteriol. 1997;179:7476-7487
    Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12.
    Tsui, H. C., G. Feng, and M. E. Winkler.
    J Bacteriol. 1998;180:2623-2629
    The L-isoaspartyl protein repair methyltransferase enhances survival of aging Escherichia coli subjected to secondary environmental stresses.
    Visick, J., H. Cai, and S. Clarke.
    J Bacteriol. 1994;176:7638-7645
    Induction of the Escherichia coli aidB gene under oxygen-limiting conditions requires a functional rpoS (katF) gene.
    Volkert, M. R., L. I. Hajec, Z. Matijasevic, F. C. Fang, and R. Prince.

    Sequences
    Amino acid
    FASTA format 
    0001 MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEQEPSDNDL AEEELLSQGA TQRVLDATQL YLGEIGYSPL 
0071 LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI EEGNLGLIRA VEKFDPERGF 
0141 RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR TARELSHKLD HEPSAEEIAE QLDKPVDDVS 
0211 RMLRLNERIT SVDTPLGGDS EKALLDILAD EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL 
0281 LGYEAATLED VGREIGLTRE RVRQIQVEGL RRLREILQTQ GLNIEALFRE
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             aatccgtaaa cccgctgcgt tatttgccgc 
-070 agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt 
0001 atgagtcaga atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg 
0071 tttttgacga aaaggcctta gtagaacagg aacccagtga taacgatttg gccgaagagg aactgttatc 
0141 gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg agattggtta ttcaccactg 
0211 ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga 
0281 tgatcgagag taacttgcgt ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc tggcgttgct 
0351 ggaccttatc gaagagggca acctggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc 
0421 cgcttctcaa catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta 
0491 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg agttgtccca 
0561 taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata agccagttga tgacgtcagc 
0631 cgtatgcttc gtcttaacga gcgcattacc tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt 
0701 tgctggacat cctggccgat gaaaaagaga acggtccgga agataccacg caagatgacg atatgaagca 
0771 gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag cgtgaagtgc tggcacgtcg attcggtttg 
0841 ctggggtacg aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc 
0911 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct 
0981 gttccgcgag taagtaagca tctgtcagaa aggccagtct caagcgaggc tggccttttc tgtgcacaat 
1051 aaaaggtccg atgcccatcg gaccttttta ttaaggtcaa att