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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
|
21
(LD)
,
DE(recA-srlR)300
,
OCL47,48-1 (LD 21_CRS)
(D)
,
OCL47,48-2 (LD 21_P/O)
(D)
,
OCL48
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
srlD
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Related strains
|
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Strains Search
|
Search strains by gene name:
srlD
Search strains by all related name:
srlD b2705 ECK2700 gutD JW2674
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General information (Go to Linear View: )
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Gene Name
|
srlD
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Alternative name
|
b2705,ECK2700,gutD,JW2674
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Location, Length
|
2,825,759 - 2,826,538
(
+
)
;
60.90
min
;
780
(bp) ,
259
(aa)
|
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Product
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sorbitol-6-phosphate dehydrogenase
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Operon Name
|
srlAEBD-gutM-srlR-gutQ
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Note
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glucitol (sorbitol)-6-phosphate dehydrogenase; GO_process: GO:0016052 - carbohydrate catabolic process
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Function
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enzyme; Degradation of small molecules: Carbon compounds
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Gene Ontology
|
GO:0003824
;
catalytic activity ( srlD )
GO:0005488
;
binding ( srlD )
GO:0008152
;
metabolic process ( srlD )
GO:0009010
;
sorbitol-6-phosphate 2-dehydrogenase activity ( srlD )
GO:0016491
;
oxidoreductase activity ( srlD )
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PID
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1789057
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EC number
(KEGG Pathway)
|
1.1.1.140
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
srlD
(
EG10971
)
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Number of removed
|
0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
|
SRLD_ECOLI
(
P05707
)
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- Protein name
|
Sorbitol-6-phosphate 2-dehydrogenase
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- Synonyms
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EC 1.1.1.140, Glucitol-6- phosphate dehydrogenase, Ketosephosphate reductase
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- Gene name
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Name=srlD; Synonyms=gutD; OrderedLocusNames=b2705;
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- Comments
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CATALYTIC ACTIVITY:D-sorbitol 6-phosphate + NAD(+) = D-fructose 6-phosphate + NADH.
PATHWAY:Sorbitol metabolism.
SUBUNIT:Homotetramer.
SIMILARITY:Belongs to the short-chain dehydrogenases/reductases (SDR) family.
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Entry name(Acc.no)
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Q8FEP0
(
Q8FEP0
)
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- Protein name
|
Sorbitol-6-phosphate 2-dehydrogenase
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- Synonyms
|
EC 1.1.1.140
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- Gene name
|
Name=srlD; OrderedLocusNames=c3259;
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- Comments
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SIMILARITY:Belongs to the short-chain dehydrogenases/reductases (SDR) family.
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Entry name(Acc.no)
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Q8X856
(
Q8X856
)
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- Protein name
|
Glucitol (Sorbitol)-6-phosphate dehydrogenase.
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- Synonyms
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- Gene name
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Name=srlD; OrderedLocusNames=z4012;
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- Comments
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SIMILARITY:Belongs to the short-chain dehydrogenases/reductases (SDR) family.
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Homology Analysis
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BLAST
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Bacteria
|
|
GTOP
|
srlD
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Oxoacyl-Acp Reductase Of Plasmodium Falciparum
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Sorbitol-6-phosphate 2-dehydrogenase
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PSI-BLAST
|
3-oxoacyl-[acyl-carrier-protein] reductase
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|
nr
(database updated :
2007.02.20
)
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BLAST
|
3-ketoacyl-(acyl-carrier-protein) reductase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
ATP_GTP_A
ADH_SHORT
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MMBR References
|
Mol Gen Genet. 1981;183(3):497-504.
Physical mapping of the srl recA region of Escherichia coli: analysis of Tn10 generated insertions and deletions.
Willis DK, Uhlin BE, Amini KS, Clark AJ.
(
6278241
)
|
J Biol Chem. 1987;262:5455-5463
Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli.
Yamada, M., and M. H. Saier.
|
J Bacteriol. 1987;169(7):2990-4.
Physical and genetic characterization of the glucitol operon in Escherichia coli.
Yamada M, Saier MH Jr.
(
3036766
)
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| Sequences |
Amino acid
FASTA format
|
0001 MNQVAVVIGG GQTLGAFLCH GLAAEGYRVA VVDIQSDKAA NVAQEINAEY GESMAYGFGA DATSEQSVLA
0071 LSRGVDEIFG RVDLLVYSAG IAKAAFISDF QLGDFDRSLQ VNLVGYFLCA REFSRLMIRD GIQGRIIQIN
0141 SKSGKVGSKH NSGYSAAKFG GVGLTQSLAL DLAEYGITVH SLMLGNLLKS PMFQSLLPQY ATKLGIKPDQ
0211 VEQYYIDKVP LKRGCDYQDV LNMLLFYASP KASYCTGQSI NVTGGQVMF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGAAGCGGAA TTTCCGGGCA CTGTCCATGT
-070 GGCAGGCCCT GTCCCCGACG ATATCGCGCC GGGATCGGTT TTGAAGTTTG AATCTGTTAA GGAGTAAAAA
0001 atgaatcagg ttgccgttgt catcggtggt gggcaaacct taggcgcgtt cctgtgccac ggtctggctg
0071 ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa
0141 cgccgaatat ggtgaaagta tggcgtacgg ttttggtgct gacgccacta gcgagcaaag cgttctggcg
0211 ctctctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta cagcgccgga atagcaaaag
0281 cagcctttat cagcgacttc cagctcggcg attttgaccg ttcgctacag gtgaatctgg tgggttattt
0351 cctgtgtgcg cgtgaatttt cgcgtttgat gatccgcgac gggattcagg ggcgcattat tcagatcaac
0421 tcgaaatccg gcaaagtggg cagcaaacac aactctggct acagcgcagc gaaatttggt ggcgtcgggc
0491 tgactcaatc actggcgctg gatctggcgg agtacggcat tacggtgcat tcactgatgc tcggtaacct
0561 gctgaaatcg ccgatgttcc agtcactgtt gccacaatac gcgaccaagc tgggtatcaa accggatcaa
0631 gtcgagcagt attacatcga caaagtaccg ctcaaacgcg gctgcgatta tcaagatgtg ctgaatatgc
0701 tgctgttcta cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc aatgtcaccg gcggtcaggt
0771 gatgttctga TCAACAGCGG AGATCCATTA AGGATCTCCG TGAGACTATA GAATGCCTGA TGCGCTACGC
0841 TCATCAGGCA TACAGGACTT CCGCCACTAC ATTAAGGAAA
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