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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(upp-guaB)92
,
OCL57
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
purM
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Related strains
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Strains Search
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Search strains by gene name:
purM
Search strains by all related name:
purM b2499 ECK2495 JW2484 purG purI
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General information (Go to Linear View: )
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Gene Name
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purM
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Alternative name
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b2499,ECK2495,JW2484,purG,purI
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Location, Length
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2,619,219 - 2,620,256
(
+
)
;
56.45
min
;
1038
(bp) ,
345
(aa)
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Product
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phosphoribosylaminoimidazole synthetase
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Operon Name
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purMN
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Note
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phosphoribosylaminoimidazole synthetase = AIR synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006164 - purine nucleotide biosynthetic process
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Function
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enzyme; Purine ribonucleotide biosynthesis
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Gene Ontology
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GO:0003824
;
catalytic activity ( purM )
GO:0004641
;
phosphoribosylformylglycinamidine cyclo-ligase activity ( purM )
GO:0005737
;
cytoplasm ( purM )
GO:0006164
;
purine nucleotide biosynthetic process ( purM )
GO:0006189
;
'de novo' IMP biosynthetic process ( purM )
GO:0016874
;
ligase activity ( purM )
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PID
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1788845
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EC number
(KEGG Pathway)
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6.3.3.1
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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purM
(
EG10798
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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PUR5_ECOLI
(
P08178
)
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- Protein name
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Phosphoribosylformylglycinamidine cyclo-ligase
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- Synonyms
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EC 6.3.3.1, AIRS, Phosphoribosyl-aminoimidazole synthetase, AIR synthase
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- Gene name
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Name=purM; Synonyms=purG; OrderedLocusNames=b2499;
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- Comments
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CATALYTIC ACTIVITY:ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
PATHWAY:De novo purine biosynthesis; fifth step.
SUBUNIT:Homodimer.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:Belongs to the AIR synthase family.
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Entry name(Acc.no)
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PUR5_ECOL6
(
Q8FF72
)
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- Protein name
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Phosphoribosylformylglycinamidine cyclo-ligase
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- Synonyms
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EC 6.3.3.1, AIRS, Phosphoribosyl-aminoimidazole synthetase, AIR synthase
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- Gene name
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Name=purM; OrderedLocusNames=c3017;
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- Comments
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CATALYTIC ACTIVITY:ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
PATHWAY:De novo purine biosynthesis; fifth step.
SUBUNIT:Homodimer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the AIR synthase family.
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Entry name(Acc.no)
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PUR5_ECO57
(
Q8XAC5
)
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- Protein name
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Phosphoribosylformylglycinamidine cyclo-ligase
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- Synonyms
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EC 6.3.3.1, AIRS, Phosphoribosyl-aminoimidazole synthetase, AIR synthase
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- Gene name
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Name=purM; OrderedLocusNames=z3762, ECs3361;
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- Comments
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CATALYTIC ACTIVITY:ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
PATHWAY:De novo purine biosynthesis; fifth step.
SUBUNIT:Homodimer (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the AIR synthase family.
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MMBR References
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J Bacteriol. 1983;154(3):1485-8.
Identification of the enzymatic reactions encoded by the purG and purI genes of Escherichia coli.
Houlberg U, Hove-Jensen B, Jochimsen B, Nygaard P.
(
6343356
)
|
J Biol Chem. 1986;261:10632-10636
Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12.
Smith, J. M., and H. A. Daum, III.
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J Biol Chem. 1987;262:10565-10569
Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12.
Smith, J. M., and H. A. Daum, III.
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| Sequences |
Amino acid
FASTA format
|
0001 MTDKTSLSYK DAGVDIDAGN ALVGRIKGVV KKTRRPEVMG GLGGFGALCA LPQKYREPVL VSGTDGVGTK
0071 LRLAMDLKRH DTIGIDLVAM CVNDLVVQGA EPLFFLDYYA TGKLDVDTAS AVISGIAEGC LQSGCSLVGG
0141 ETAEMPGMYH GEDYDVAGFC VGVVEKSEII DGSKVSDGDV LIALGSSGPH SNGYSLVRKI LEVSGCDPQT
0211 TELDGKPLAD HLLAPTRIYV KSVLELIEKV DVHAIAHLTG GGFWENIPRV LPDNTQAVID ESSWQWPEVF
0281 NWLQTAGNVE HHEMYRTFNC GVGMIIALPA PEVDKALALL NANGENAWKI GIIKASDSEQ RVVIE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AAAAGGTTGT GTAAAGCAGT CTCGCAAACG
-070 TTTGCTTTCC CTGTTAGAAT TGCGCCGAAT TTTATTTTTC TACCGCAAGT AACGCGTGGG GACCCAAGCA
0001 gtgaccgata aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
0071 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc
0141 gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttctggca ctgacggcgt aggtaccaag
0211 ctgcgtctgg caatggactt aaaacgtcac gacaccattg gtattgatct ggtcgccatg tgcgttaatg
0281 acctggtggt gcaaggtgca gagccgctgt ttttcctcga ctattacgca accggaaaac tggatgttga
0351 taccgcttca gcggtgatca gcggcattgc ggaaggttgt ctgcaatcag gctgttcact ggtgggtggc
0421 gaaacggcag aaatgccggg gatgtatcac ggtgaggatt acgatgtcgc gggtttctgc gttggcgtgg
0491 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag
0561 cggtccacac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga tccgcaaacc
0631 accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg catttacgtg aagtcagtgc
0701 tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca cctgaccggc ggcggcttct gggaaaacat
0771 tccgcgcgta ttgccagata atactcaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc
0841 aactggctgc aaacggcagg taacgttgag caccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
0911 tgattattgc cctgcctgct ccggaagtgg acaaagccct cgccctgctc aatgccaacg gtgaaaacgc
0981 gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataaTG AATATTGTGG
1051 TGCTTATTTC CGGCAACGGA AGTAATTTAC AGGCAATTAT TGACGCCTGT AAAACCAACA AAATTAAAGG
1121 CACCGTACGG GCAGTTTT
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