MG1655
W3110
Search for
Japanese | English
Top Genes Deletions Circular Linear Motif Contigs Reference SequenceCutter Download Strains TEC
Gene Report : glpB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
J Bacteriol. 1990;172(6):3191-200.
Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.
Metcalf WW, Steed PM, Wanner BL. ( 2160940 )
     Deletion 13 (LD)  ,  OCL67,68-3 (LD 13B_CRS) (D)  ,  OCL67,68-4 (LD 13B_PO) (D)  ,  OCL68 (D)  ,  OCL68,69-10 (BK9-6 / AK7-6) (D)  ,  OCL68,69-9 (BK9-6 / AK6-6) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  glpB
Related strains
     Strains Search Search strains by gene name:  glpB   Search strains by all related name:  glpB b2242 ECK2234 glpC JW2236 psi-51

General information  (Go to Linear View:)
 Gene Name glpB  
 Alternative name b2242,ECK2234,glpC,JW2236,psi-51  
 Location, Length 2,352,287 - 2,353,546 (  +  ) ;   50.70 min ; 1260 (bp) ,   419 (aa) Go to Linear View
 Product sn-glycerol-3-phosphate dehydrogenase (anaerobic), membrane anchor subunit  
 Operon Name glpABC  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0006071 - glycerol metabolic process  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0004368 ; glycerol-3-phosphate dehydrogenase activity ( glpB )
GO:0005506 ; iron ion binding ( glpC )
GO:0005886 ; plasma membrane ( glpB )
GO:0005886 ; plasma membrane ( glpC )
GO:0006118 ; electron transport ( glpB )
GO:0006118 ; electron transport ( glpC )
GO:0006810 ; transport ( glpC )
GO:0009055 ; electron carrier activity ( glpC )
GO:0016020 ; membrane ( glpB )
GO:0016020 ; membrane ( glpC )
GO:0016491 ; oxidoreductase activity ( glpB )
GO:0016491 ; oxidoreductase activity ( glpC )
GO:0046872 ; metal ion binding ( glpC )
GO:0051536 ; iron-sulfur cluster binding ( glpC )
GO:0051539 ; 4 iron, 4 sulfur cluster binding ( glpC )
 PID 1788575  
 EC number
  (KEGG Pathway)   		1.1.5.3  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene glpB ( EG10392 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) GLPB_ECOLI ( P13033 )
    -  Protein name Anaerobic glycerol-3-phosphate dehydrogenase subunit B  
    -  Synonyms EC 1.1.99.5, Anaerobic G-3-P dehydrogenase subunit B, Anaerobic G3Pdhase B  
    -  Gene name Name=glpB; OrderedLocusNames=b2242;  
    -  Comments
  • FUNCTION:Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.
  • CATALYTIC ACTIVITY:Sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor.
  • COFACTOR:FMN is said to be associated with glpB.
  • PATHWAY:First step in the transfer of reducing equivalents from G-3-P to a short electron transfer chain terminating with fumarate as the ultimate electron acceptor.
  • SUBUNIT:Composed of a catalytic glpA/B dimer and of membrane bound glpC.
  • SUBCELLULAR LOCATION:Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.
  • SIMILARITY:Belongs to the anaerobic G-3-P dehydrogenase subunit B family.  
    •   botton Entry name(Acc.no) GLPB_ECOL6 ( Q8FFN4 )
        -  Protein name Anaerobic glycerol-3-phosphate dehydrogenase subunit B  
        -  Synonyms EC 1.1.99.5, Anaerobic G-3-P dehydrogenase subunit B, Anaerobic G3Pdhase B  
        -  Gene name Name=glpB; OrderedLocusNames=c2783;  
        -  Comments
  • FUNCTION:Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor (By similarity).
  • CATALYTIC ACTIVITY:Sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor.
  • COFACTOR:FMN is said to be associated with glpB (By similarity).
  • PATHWAY:First step in the transfer of reducing equivalents from G-3-P to a short electron transfer chain terminating with fumarate as the ultimate electron acceptor.
  • SUBUNIT:Composed of a catalytic glpA/B dimer and of membrane bound glpC (By similarity).
  • SUBCELLULAR LOCATION:Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase (By similarity).
  • SIMILARITY:Belongs to the anaerobic G-3-P dehydrogenase subunit B family.  
    •   botton Entry name(Acc.no) GLPB_ECO57 ( Q8XE13 )
        -  Protein name Anaerobic glycerol-3-phosphate dehydrogenase subunit B  
        -  Synonyms EC 1.1.99.5, Anaerobic G-3-P dehydrogenase subunit B, Anaerobic G3Pdhase B  
        -  Gene name Name=glpB; OrderedLocusNames=z3500, ECs3127;  
        -  Comments
  • FUNCTION:Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor (By similarity).
  • CATALYTIC ACTIVITY:Sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor.
  • COFACTOR:FMN is said to be associated with glpB (By similarity).
  • PATHWAY:First step in the transfer of reducing equivalents from G-3-P to a short electron transfer chain terminating with fumarate as the ultimate electron acceptor.
  • SUBUNIT:Composed of a catalytic glpA/B dimer and of membrane bound glpC (By similarity).
  • SUBCELLULAR LOCATION:Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase (By similarity).
  • SIMILARITY:Belongs to the anaerobic G-3-P dehydrogenase subunit B family.  

    •  Linear View (Whole Mode)
    View Location
       2340.0  –  2365.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP glpB (  homologous genes of other bacterias  )   glpC (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of Thioredoxin Reductase-Related Protein Ttha0370 From Thermus Thermophilus Hb8  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Anaerobic glycerol-3-phosphate dehydrogenase subunit B  
             PSI-BLAST tRNA uridine 5-carboxymethylaminomethyl modification enzyme gidA
        nr     (database updated : 2007.02.20 )
             BLAST anaerobic glycerol-3-phosphate dehydrogenase subunit B [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        COG COG3075E 
        EcoCyc glpB 

    MMBR References
    J Bacteriol. 1987;169(2):526-32.
    Divergent transcription of the sn-glycerol-3-phosphate active transport (glpT) and anaerobic sn-glycerol-3-phosphate dehydrogenase (glpA glpC glpB) genes of Escherichia coli K-12.
    Ehrmann M, Boos W, Ormseth E, Schweizer H, Larson TJ. ( 3027032 )
    Biochem Cell Biol. 1995;73(3-4):147-53.
    Physiological role of GlpB of anaerobic glycerol-3-phosphate dehydrogenase of Escherichia coli.
    Varga ME, Weiner JH. ( 7576488 )
    J Bacteriol. 1988;170(6):2448-56.
    Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12.
    Cole ST, Eiglmeier K, Ahmed S, Honore N, Elmes L, Anderson WF, Weiner JH. ( 3286606 )

    Sequences
    Amino acid
    FASTA format 
    0001 MRFDTVIMGG GLAGLLCGLQ LQKHGLRCAI VTRGQSALHF SSGSLDLLSH LPDGQPVTDI HSGLESLRQQ 
0071 APAHPYSLLE PQRVLDLACQ AQALIAESGA QLQGSVELAH QRVTPLGTLR STWLSSPEVP VWPLPAKKIC 
0141 VVGISGLMDF QAHLAAASLR ELGLAVETAE IELPELDVLR NNATEFRAVN IARFLDNEEN WPLLLDALIP 
0211 VANTCEMILM PACFGLADDK LWRWLNEKLP CSLMLLPTLP PSVLGIRLQN QLQRQFVRQG GVWMPGDEVK 
0281 KVTCKNGVVN EIWTRNHADI PLRPRFAVLA SGSFFSGGLV AERNGIREPI LGLDVLQTAT RGEWYKGDFF 
0351 APQPWQQFGV TTDETLRPSQ AGQTIENLFA IGSVLGGFDP IAQGCGGGVC AVSALHAAQQ IAQRAGGQQ
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             AAAGGCGTGC AACCCATCGC CTGGGGAGAT 
-070 GCACTGCGCG AAAGCGAATT TACCCGCTGG GTTTATCAGG GATTGTGTGG TCTGGAGAAG GAGCAGAAAG 
0001 atgcgctttg atactgtcat tatgggcggc ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac 
0071 acggcctgcg ctgtgccatt gtcactcgtg gtcaaagcgc actgcatttc tcatccggat cgctggattt 
0141 gctgagccat ctgccagatg gtcaaccggt gacagacatt cacagtggac tggaatcttt gcgtcagcag 
0211 gcaccagccc atccttactc ccttctcgag ccacaacgcg tgctcgatct cgcttgccag gcgcaggcat 
0281 taatcgctga aagcggtgcg caattgcagg gcagcgtaga acttgctcac cagcgggtta cgccgctcgg 
0351 cactctgcgc tctacctggc taagttcgcc agaagtcccc gtctggccgc tgcccgcgaa gaaaatatgt 
0421 gtagtgggaa ttagcggcct gatggatttt caggcgcacc ttgcggcagc ttcgttgcgt gaactcggcc 
0491 ttgccgttga aaccgcagaa atagagctgc cggaactgga tgtgctgcgc aataacgcca ccgaatttcg 
0561 cgcggtgaat atcgcccgtt tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct 
0631 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggtctggc cgatgacaaa ctgtggcgtt 
0701 ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg ccttccgtgc tgggcattcg 
0771 tctgcaaaac cagttacagc gccagtttgt gcgccagggt ggcgtgtgga tgccgggcga tgaagtgaaa 
0841 aaagtgacct gtaaaaatgg cgtagtgaac gaaatctgga cccgcaatca cgccgatatt ccgctacgtc 
0911 cacgtttcgc ggttctcgcc agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg 
0981 agagccgatt ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg agattttttt 
1051 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcacag gcagggcaaa 
1121 ccattgaaaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc atcgcccagg gatgcggcgg 
1191 cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag attgcccaac gcgcaggagg ccaacaatga 
1261 ATGACACCAG CTTCGAAAAC TGCATTAAGT GCACCGTCTG CACCACCGCC TGCCCGGTGA GCCGGGTGAA 
1331 TCCCGGTTAT CCAGGGCCAA AACAAGCCGG