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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Mol Gen Genet. 1994;242(6):744-8.
Effect of ogt expression on mutation induction by methyl-, ethyl- and propylmethanesulphonate in Escherichia coli K12 strains.
Abril N, Hera C, Alejandre E, Rafferty JA, Margison GP, Pueyo C.
(
8152424
)
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Deletion
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14
(LD)
,
OCL71
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
ada
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Related strains
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Strains Search
|
Search strains by gene name:
ada
Search strains by all related name:
ada b2213 ECK2205 JW2201
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General information (Go to Linear View: )
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Gene Name
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ada
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Alternative name
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b2213,ECK2205,JW2201
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Location, Length
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2,307,363 - 2,308,427
(
-
)
;
49.73
min
;
1065
(bp) ,
354
(aa)
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Product
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fused DNA-binding transcriptional dualregulator/O6-methylguanine-DNA methyltransferase
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Operon Name
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ada-alkB
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Note
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O6-methylguanine-DNA methyltransferase;transcription activator/repressor; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0006281 - DNA repair; GO_process: GO:0006350 - transcription
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003677
;
DNA binding ( ada )
GO:0003700
;
transcription factor activity ( ada )
GO:0003824
;
catalytic activity ( ada )
GO:0003908
;
methylated-DNA-[protein]-cysteine S-methyltransferase activity ( ada )
GO:0005622
;
intracellular ( ada )
GO:0006281
;
DNA repair ( ada )
GO:0006350
;
transcription ( ada )
GO:0006355
;
regulation of transcription, DNA-dependent ( ada )
GO:0006974
;
response to DNA damage stimulus ( ada )
GO:0008168
;
methyltransferase activity ( ada )
GO:0008270
;
zinc ion binding ( ada )
GO:0016740
;
transferase activity ( ada )
GO:0043565
;
sequence-specific DNA binding ( ada )
GO:0046872
;
metal ion binding ( ada )
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PID
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1788542
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EC number
(KEGG Pathway)
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2.1.1.63
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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ada
(
EG10029
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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ADA_ECOLI
(
P06134
;
Q47032
)
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- Protein name
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ADA regulatory protein
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- Synonyms
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Regulatory protein of adaptative response
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- Gene name
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Name=ada; OrderedLocusNames=b2213;
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- Comments
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FUNCTION:Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine.
FUNCTION:The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB.
CATALYTIC ACTIVITY:DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
COFACTOR:One zinc ion per subunit.
SIMILARITY:The regulatory part belongs to the araC/xylS family of transcriptional regulators.
SIMILARITY:In the C-terminal section; belongs to the MGMT family.
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Entry name(Acc.no)
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Q8CXY7
(
Q8CXY7
)
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- Protein name
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ADA Regulatory protein
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- Synonyms
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EC 2.1.1.63
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- Gene name
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Name=ada; OrderedLocusNames=c2754;
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- Comments
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SIMILARITY:Contains 1 HTH araC/xylS-type DNA-binding domain.
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Entry name(Acc.no)
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Q8XE42
(
Q8XE42
)
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- Protein name
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O6-methylguanine-DNA methyltransferase; transcription activator/repressor.
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- Synonyms
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- Gene name
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Name=ada; OrderedLocusNames=z3471;
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- Comments
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SIMILARITY:Contains 1 HTH araC/xylS-type DNA-binding domain.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
ada
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain , Ada O6-Methylguanine-Dna Methyltransferase From Escherichia Coli
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Regulatory protein ada
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PSI-BLAST
|
Regulatory protein ada
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|
nr
(database updated :
2007.02.20
)
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BLAST
|
Ada regulatory protein
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
MYRISTYL
RGD
HTH_ARAC_FAMILY_1
MGMT
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MMBR References
|
Nature. 1982;296(5859):773-5.
Adaptation to alkylation resistance involves the induction of a DNA glycosylase.
Evensen G, Seeberg E.
(
7040984
)
|
J Bacteriol. 1979;139(3):783-91.
Isolation and characterization of Escherichia coli K-12 mutants unable to induce the adaptive response to simple alkylating agents.
Jeggo P.
(
383692
)
|
Mol Gen Genet. 1985;198(2):263-9.
Molecular cloning and characterization of the alkB gene of Escherichia coli.
Kataoka H, Sekiguchi M.
(
3884973
)
|
EMBO J. 1995;14:4329-4335
RNA polymerase alpha subunit binding site in positively controlled promoters: a new model for RNA polymerase-promoter interaction and transcriptional activation in the Escherichia coli ada and aidB genes.
Landini, P., and M. R. Volkert.
|
J Bacteriol. 1985;161(3):888-95.
Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents.
Lemotte PK, Walker GC.
(
2982792
)
|
J Bacteriol. 1994;176:3224-3230
DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli.
Mackay, W. J., S. Han, and L. D. Samson.
|
Nucleic Acids Res. 1985;13:1939-1952
Cloning of the E. coli O6-methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay.
Margison, G. P., D. P. Cooper, and J. Brennand.
|
J Bacteriol. 1982;152(1):534-7.
O6-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli.
Mitra S, Pal BC, Foote RS.
(
6749819
)
|
J Biol Chem. 1998;273:15157-15161
Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity.
Muzzin, O., E. A. Campbell, L. Xia, E. Severinova, S. A. Darst, and K. Severinov.
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J Biol Chem. 1985;260:7281-7288
Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methyl-guanine-DNA methyltransferase.
Nakabeppu, Y., H. Kondo, S. I. Kawabata, S. Iwanaga, and M. Sekiguchi.
|
J Bacteriol. 1995;177:1268-1274
Alteration of lysine 178 in the hinge region of the Escherichia coli Ada protein interferes with activation of ada, but not alkA, transcription.
Saget, B. M., D. E. Shevell, and G. C. Walker.
|
J. Biochem. (Tokyo) 1995;118:1184-1191
Sequence-specific DNA recognition of the Escherichia coli Ada protein associated with the methylation-dependent functional switch for transcriptional regulation.
Sakashita, H., T. Sakuma, Y. Akitomo, T. Ohkubo, M. Kainosho, M. Sekiguchi, and K. Morikawa.
|
J Bacteriol. 1993;175:2455-2457
The Ada protein is a class I transcription factor of Escherichia coli.
Sakumi, K., K. Igarashi, M. Sekiguchi, and A. Ishihama.
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J Bacteriol. 1982;150(2):984-8.
Genetic mapping of ada and adc mutations affecting the adaptive response of Escherichia coli to alkylating agents.
Sedgwick B.
(
7040349
)
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Mol Gen Genet. 1983;191(3):466-72.
Molecular cloning of a gene which regulates the adaptive response to alkylating agents in Escherichia coli.
Sedgwick B.
(
6355769
)
|
Mol Gen Genet. 1980;180(1):85-90.
Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: mutants deficient in thiols and mutants constitutive for the adaptive response.
Sedgwick B, Robins P.
(
7003313
)
|
Mutat Res. 1991;254(1):37-44.
Roles of two types of O6-methylguanine-DNA methyltransferases in DNA repair.
Takano K, Nakamura T, Sekiguchi M.
(
1986273
)
|
Cell. 1986;45(2):315-24.
The intracellular signal for induction of resistance to alkylating agents in E. coli.
Teo I, Sedgwick B, Kilpatrick MW, McCarthy TV, Lindahl T.
(
3009022
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE ALAAGFRPCK
0071 RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF KATTGMTPKA WQQAWRARRL
0141 RESLAKGESV TTSILNAGFP DSSSYYRKAD ETLGMTAKQF RHGGENLAVR YALADCELGR CLVAESERGI
0211 CAILLGDDDA TLISELQQMF PAADNAPADL MFQQHVREVI ASLNQRDTPL TLPLDIRGTA FQQQVWQALR
0281 TIPCGETVSY QQLANAIGKP KAVRAVASAC AANKLAIIIP CHRVVRGDGT LSGYRWGVSR KAQLLRREAE
0351 NEER
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 agcttccttg tcagcgaaaa aaattaaagc
-070 gcaagattgt tggtttttgc gtgatggtga ccgggcagcc taaaggctat ccttaaccag ggagctgatt
0001 atgaaaaaag ccacatgctt aactgacgat caacgctggc aatctgtctt agcccgcgac ccgaatgccg
0071 acggcgaatt cgttttcgcc gtgcgtacca caggcatctt ttgccgtccg tcttgccgcg ccagacatgc
0141 tttgcgggaa aacgtctcct tctacgcaaa tgccagcgag gcactcgccg ctggctttcg cccctgcaaa
0211 cgttgtcagc cagaaaaagc caatgcccag caacatcggt tggataaaat cacccacgcg tgtcgactgc
0281 tggaacagga aacgcctgta acgctggaag ccttagccga ccaggtggcg atgagtccat ttcatctaca
0351 tcggttgttt aaagcgacta ccggaatgac gcctaaagcc tggcaacagg cctggcgcgc tcgccgtttg
0421 cgcgaatcgc tggcgaaagg ggagagcgtg acgacgtcta ttcttaacgc cggattcccc gacagcagca
0491 gttactatcg caaagctgac gaaacgctgg gcatgacggc taaacaattc cgtcacggtg gcgaaaatct
0561 ggcggtgcgt tacgcgctgg ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt
0631 tgcgcgatat tgctgggcga tgatgacgcg acactaatca gcgagttgca gcagatgttt cccgctgccg
0701 acaacgcgcc tgccgatctg atgtttcagc aacatgtgcg tgaagtgatc gccagcctca atcaacgcga
0771 tacgccgctg acgttaccgc tggacattcg cggcactgct tttcagcaac aagtctggca ggcactgcgc
0841 acgatacctt gcggtgaaac cgtcagttat cagcaactgg ctaacgccat cggcaaaccg aaagcggtac
0911 gggccgttgc cagcgcctgt gccgccaaca agctggctat cataataccc tgtcatcggg tggtccgtgg
0981 tgatggcaca ctttccggtt accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa
1051 aatgaggaga ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg cggctggtgc
1121 ggtaatttta cggcgttttg cttttaacgc tgcggagcaa ctgat
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