MG1655
W3110
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Gene Report : fadE
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR36 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  fadE
Related strains
     Strains Search Search strains by gene name:  fadE   Search strains by all related name:  fadE b0221 ECK0222 f826 fadF JW5020 yafH

General information  (Go to Linear View:)
 Gene Name fadE  
 Alternative name b0221,ECK0222,f826,fadF,JW5020,yafH  
 Location, Length 240,859 - 243,303 (  -  ) ;   5.19 min ; 2445 (bp) ,   814 (aa) Go to Linear View
 Product acyl coenzyme A dehydrogenase  
 Operon Name fadE  
 Note GO_process: GO:0019395 - fatty acid oxidation  
 Function putative enzyme; Not classified  
 Gene Ontology GO:0003995 ; acyl-CoA dehydrogenase activity ( fadE )
GO:0006118 ; electron transport ( fadE )
GO:0006629 ; lipid metabolic process ( fadE )
GO:0006631 ; fatty acid metabolic process ( fadE )
GO:0008152 ; metabolic process ( fadE )
GO:0016491 ; oxidoreductase activity ( fadE )
GO:0016627 ; oxidoreductase activity, acting on the CH-CH group of donors ( fadE )
GO:0050660 ; FAD binding ( fadE )
 PID 87081702  
 EC number
  (KEGG Pathway)   		1.3.99.-  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) FADE_ECOLI ( Q47146 ; P71283; P75673; Q47680 )
    -  Protein name Acyl-coenzyme A dehydrogenase  
    -  Synonyms EC 1.3.99.-, ACDH  
    -  Gene name Name=fadE; OrderedLocusNames=b0221;  
    -  Comments
  • FUNCTION:Catalyzes the dehydrogenation of acyl-CoA.
  • CATALYTIC ACTIVITY:An acyl-CoA + FAD = a dehydrogenated acyl-CoA + FADH(2).
  • COFACTOR:FAD.
  • PATHWAY:Fatty acid beta-oxidation cycle; first step.
  • INDUCTION:Negatively regulated by fadR.
  • SIMILARITY:Belongs to the acyl-CoA dehydrogenase family.
  • CAUTION:Originally this gene was mistakenly named fadF.  
    •   botton Entry name(Acc.no) Q8FKN6 ( Q8FKN6 )
        -  Protein name Acyl-coenzyme A dehydrogenase  
        -  Synonyms EC 1.3.99.-  
        -  Gene name Name=yafH; OrderedLocusNames=c0371;  
        -  Comments
  • SIMILARITY:Belongs to the acyl-CoA dehydrogenase family.  

    •  Linear View (Whole Mode)
    View Location
       230.0  –  255.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP fadE (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of Rat Short Chain Acyl-Coa Dehydrogenase Complexed With Acetoacetyl-Coa  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Acyl-coenzyme A dehydrogenase  
             PSI-BLAST Acyl-coenzyme A dehydrogenase
        nr     (database updated : 2007.02.20 )
             BLAST probable membrane protein b0221 - Escherichia coli  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    LEUCINE_ZIPPER     

    Other Cross-Reference
        COG COG1960I 
        EcoCyc fadE 

    MMBR References
    J Bacteriol. 1981;148(2):521-6.
    Regulation of fatty acid degradation in Escherichia coli: analysis by operon fusion.
    Clark D. ( 6271734 )

    Sequences
    Amino acid
    FASTA format 
    0001 MMILSILATV VLLGALFYHR VSLFISSLIL LAWTAALGVA GLWSAWVLVP LAIILVPFNF APMRKSMISA 
0071 PVFRGFRKVM PPMSRTEKEA IDAGTTWWEG DLFQGKPDWK KLHNYPQPRL TAEEQAFLDG PVEEACRMAN 
0141 DFQITHELAD LPPELWAYLK EHRFFAMIIK KEYGGLEFSA YAQSRVLQKL SGVSGILAIT VGVPNSLGPG 
0211 ELLQHYGTDE QKDHYLPRLA RGQEIPCFAL TSPEAGSDAG AIPDTGIVCM GEWQGQQVLG MRLTWNKRYI 
0281 TLAPIATVLG LAFKLSDPEK LLGGAEDLGI TCALIPTTTP GVEIGRRHFP LNVPFQNGPT RGKDVFVPID 
0351 YIIGGPKMAG QGWRMLVECL SVGRGITLPS NSTGGVKSVA LATGAYAHIR RQFKISIGKM EGIEEPLARI 
0421 AGNAYVMDAA ASLITYGIML GEKPAVLSAI VKYHCTHRGQ QSIIDAMDIT GGKGIMLGQS NFLARAYQGA 
0491 PIAITVEGAN ILTRSMMIFG QGAIRCHPYV LEEMEAAKNN DVNAFDKLLF KHIGHVGSNK VRSFWLGLTR 
0561 GLTSSTPTGD ATKRYYQHLN RLSANLALLS DVSMAVLGGS LKRRERISAR LGDILSQLYL ASAVLKRYDD 
0631 EGRNEADLPL VHWGVQDALY QAEQAMDDLL QNFPNRVVAG LLNVVIFPTG RHYLAPSDKL DHKVAKILQV 
0701 PNATRSRIGR GQYLTPSEHN PVGLLEEALV DVIAADPIHQ RICKELGKNL PFTRLDELAH NALVKGLIDK 
0771 DEAAILVKAE ESRLRSINVD DFDPEELATK PVKLPEKVRK VEAA
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             ttgtaattat attgtaaaca tattgctaaa 
-070 tgtttttaca tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt 
0001 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat 
0071 ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct ggtctgtggt cggcgtgggt 
0141 actggtgcct ctggccatta tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat gatttccgcg 
0211 ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg 
0281 gcaccacctg gtgggagggc gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca 
0351 gccgcgcctg accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat 
0421 gatttccaga tcacccatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgtt 
0491 tcttcgcgat gatcatcaaa aaagagtacg gcgggctgga gttctcggct tatgcccagt ctcgcgtgct 
0561 gcaaaaactc tccggcgtga gcgggatcct ggcgattacc gtcggcgtgc caaactcatt aggcccgggc 
0631 gaactgttgc aacattacgg cactgacgag cagaaagatc actatctgcc gcgtctggcg cgtggtcagg 
0701 agatcccctg ctttgcactg accagcccgg aagcgggttc cgatgcgggc gcgattccgg acaccgggat 
0771 tgtctgcatg ggcgaatggc agggccagca ggtgctgggg atgcgtctga cctggaacaa acgctacatt 
0841 acgctggcac cgattgcgac cgtgcttggg ctggcgttta aactctccga cccggaaaaa ttactcggcg 
0911 gtgcagaaga tttaggcatt acctgtgcgc tgatcccaac caccacgccg ggcgtggaaa ttggtcgtcg 
0981 ccacttcccg ctgaacgtac cgttccagaa cggaccgacg cgcggtaaag atgtcttcgt gccgatcgat 
1051 tacatcatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt ggagtgcctc tcggtaggcc 
1121 gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa atcggtagcg ctggcaaccg gcgcgtatgc 
1191 tcacattcgc cgtcagttca aaatctctat tggtaagatg gaagggattg aagagccgct ggcgcgtatt 
1261 gccggtaatg cctacgtgat ggatgctgcg gcatcgctga ttacctacgg cattatgctc ggcgaaaaac 
1331 ctgccgtgct gtcggctatc gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat 
1401 ggatattacc ggcggtaaag gcattatgct cgggcaaagc aacttcctgg cgcgtgctta ccagggcgca 
1471 ccgattgcca tcaccgttga aggggctaac attctgaccc gcagcatgat gatcttcgga caaggagcga 
1541 ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc gaagaacaat gacgtcaacg cgttcgataa 
1611 actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg cctgacgcgc 
1681 ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca gcacctgaac cgcctgagcg 
1751 ccaacctcgc cctgctttct gatgtctcga tggcagtgct gggcggcagc ctgaaacgtc gcgagcgcat 
1821 ctcggcccgt ctgggggata ttttaagcca gctctacctc gcctctgccg tgctgaagcg ttatgacgac 
1891 gaaggccgta atgaagccga cctgccgctg gtgcactggg gcgtacaaga tgcgctgtat caggctgaac 
1961 aggcgatgga tgatttactg caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt 
2031 cccgaccgga cgtcattatc tggcaccttc tgacaagctg gatcataaag tggcgaagat tttacaagtg 
2101 ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc tgacgccgag cgagcataat ccggttggct 
2171 tgctggaaga ggcgctggtg gatgtgattg ccgccgaccc aattcatcag cggatctgta aagagctggg 
2241 taaaaacctg ccgtttaccc gtctggatga actggcgcac aacgcgctgg tgaaggggct gattgataaa 
2311 gatgaagccg ctattctggt gaaagctgaa gaaagccgtc tgcgcagtat taacgttgat gactttgatc 
2381 cggaagagct ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa gttgaagccg cgtaaatgaa 
2451 taacggagcc gaaaggctcc gtttctttat ccgctaatta tttaaaatta aagccatccg gatggttttc 
2521 caggctgccg gtcaacgccg cgaac