MG1655
W3110
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Gene Report : nhaR
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
J Exp Biol. 1994;196:443-56.
Molecular physiology of the Na+/H+ antiporter in Escherichia coli.
Padan E, Schuldiner S. ( 7823039 )
     Deletion OCR27 (D)  ,  OCR27-8 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  nhaR
Related strains
     Strains Search Search strains by gene name:  nhaR   Search strains by all related name:  nhaR antO b0020 ECK0021 JW0019 yaaB

General information  (Go to Linear View:)
 Gene Name nhaR  
 Alternative name antO,b0020,ECK0021,JW0019,yaaB  
 Location, Length 18,715 - 19,620 (  +  ) ;   0.40 min ; 906 (bp) ,   301 (aa) Go to Linear View
 Product DNA-binding transcriptional activator  
 Operon Name nhaAR  
 Note transcriptional activator of nhaA; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_process: GO:0006350 - transcription; GO_process: GO:0009268 - response to pH  
 Function regulator; Transport of small molecules: Cations  
 Gene Ontology GO:0003677 ; DNA binding ( nhaR )
GO:0003700 ; transcription factor activity ( nhaR )
GO:0005515 ; protein binding ( nhaR )
GO:0005737 ; cytoplasm ( nhaR )
GO:0006350 ; transcription ( nhaR )
GO:0006355 ; regulation of transcription, DNA-dependent ( nhaR )
 PID 1786202  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) NHAR_ECOLI ( P10087 ; P75619; Q47409; Q83SR2 )
    -  Protein name Transcriptional activator protein nhaR  
    -  Synonyms Na+/H+ antiporter regulatory protein  
    -  Gene name Name=nhaR; Synonyms=antO; OrderedLocusNames=b0020, SF0017, S0019;  
    -  Comments
  • FUNCTION:Plays a role in the positive regulation of nhaA.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Contains 1 HTH lysR-type DNA-binding domain.  
    •   botton Entry name(Acc.no) Q8FLC0 ( Q8FLC0 )
        -  Protein name Transcriptional activator protein nhaR.  
        -  Synonyms  
        -  Gene name Name=nhaR; OrderedLocusNames=c0025;  
        -  Comments
  • SIMILARITY:Belongs to the ATP-dependent AMP-binding enzyme family.  
    •   botton Entry name(Acc.no) Q8X4Y0 ( Q8X4Y0 )
        -  Protein name Transcriptional activator of nhaA.  
        -  Synonyms  
        -  Gene name Name=nhaR; OrderedLocusNames=z0019;  
        -  Comments
  • SIMILARITY:Contains 1 HTH lysR-type DNA-binding domain.  

    •  Linear View (Whole Mode)
    View Location
       10.0  –  35.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP nhaR (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain , Cofactor Binding Fragment Of Cysb From Klebsiella Aerogenes  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Transcriptional activator protein nhaR (Na(+)/H(+) antiporter regulatory protein)  
             PSI-BLAST Transcriptional activator protein nhaR (Na(+)/H(+) antiporter regulatory protein)
        nr     (database updated : 2007.02.20 )
             BLAST DNA-binding transcriptional activator [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    HTH_LYSR_FAMILY     

    Other Cross-Reference
        COG COG0583K 
        EcoCyc nhaR 

    MMBR References
    J Bioenerg Biomembr. 1993;25(6):647-69.
    Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells.
    Padan E, Schuldiner S. ( 8144493 )
    J Biol Chem. 1992;267:10433-10438
    NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR) regulates nhaA, the sodium proton antiporter gene in Escherichia coli.
    Rahav-Manor, O., O. Carmel, R. Karpel, D. Taglicht, G. Glaser, S. Schuldiner, and E. Padan.
    EMBO J. 1994;13:1981-1989
    A single amino acid substitution (Glu134Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli.
    Carmel, O., N. Dover, O. Rahav-Manor, P. Dibrov, D. Kirsch, R. Karpel, S. Schuldiner, and E. Padan.

    Sequences
    Amino acid
    FASTA format 
    0001 MSMSHINYNH LYYFWHVYKE GSVVGAAEAL YLTPQTITGQ IRALEERLQG KLFKRKGRGL EPSELGELVY 
0071 RYADKMFTLS QEMLDIVNYR KESNLLFDVG VADALSKRLV SSVLNAAVVE GEPIHLRCFE STHEMLLEQL 
0141 SQHKLDMIIS DCPIDSTQQE GLFSVRIGEC GVSFWCTNPP PEKPFPACLE ERRLLIPGRR SMLGRKLLNW 
0211 FNSQGLNVEI LGEFDDAALM KAFGAMHNAI FVAPTLYAYD FYADKTVVEI GRVENVMEEY HAIFAERMIQ 
0281 HPAVQRICNT DYSALFSPAV R
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             GATACAGCTG GTTACGCGTT CGTTTGCGTC 
-070 CATCAGTTTG ACAGGACGGT TTACCGGGGA GCCATAAACG GCTCCCTTTT CATTGTTATC AGGGAGAGAA 
0001 atgagcatgt ctcatatcaa ttacaaccac ttgtattact tctggcatgt ctataaagaa ggttccgtgg 
0071 ttggcgcagc ggaggcgctt tatttaactc cacaaaccat taccggacag attcgagcgc tggaagagcg 
0141 cctgcaaggc aaattattta aacgcaaggg acgtggtctc gaacccagcg agctgggaga actggtctat 
0211 cgctatgccg ataaaatgtt caccttaagc caggaaatgc tggatattgt gaactatcgc aaagaatcca 
0281 atttattgtt tgacgttggc gtggctgatg cactttccaa acgcctggtc agtagcgtac ttaacgccgc 
0351 agtggtagaa ggcgagccca ttcatcttcg ctgcttcgaa tccacccacg aaatgctgct ggagcaatta 
0421 agtcagcata aactggatat gatcatttct gactgtccga tagactctac gcagcaggaa ggcctgttct 
0491 ccgtgagaat tggcgaatgt ggcgtgagtt tctggtgtac aaatccacca ccagaaaaac cgttcccggc 
0561 ttgtctggaa gaacggcgac ttttgattcc tgggcgacgt tcaatgttag ggcgcaaatt gcttaactgg 
0631 tttaactccc agggattaaa cgtagaaatc ctcggcgagt ttgatgatgc cgctttgatg aaagcttttg 
0701 gtgcgatgca caatgcaatc ttcgttgccc caacgcttta tgcatatgac ttttatgccg ataaaactgt 
0771 cgtagaaatt ggtcgcgtcg agaatgtgat ggaagagtac catgctattt ttgctgagcg gatgattcag 
0841 cacccggcgg tacagcgaat ctgcaatacg gattattctg cgctttttag tccagcggtg cgttaaTCGG 
0911 CAGCTCCCCC AAAGTTAAGG TGGGGGAGAT AGATTAGTTG TACATTACCA CGATTTTGAC TCGGCTCATT 
0981 ATTTGCCCGC TTGAGACATT GTTTCC