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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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16-1-2
(LD)
,
DE(sbcB-rfb)202
,
DE(hisG-hisI)870
,
OCL74-2,75-5 (LD 16-1-2_CRS)
(D)
,
OCL74-2,75-6 (LD 16-1-2_P/O)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
hisF
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Related strains
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Strains Search
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Search strains by gene name:
hisF
Search strains by all related name:
hisF b2025 ECK2020 JW2007
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General information (Go to Linear View: )
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Gene Name
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hisF
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Alternative name
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b2025,ECK2020,JW2007
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Location, Length
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2,093,868 - 2,094,644
(
+
)
;
45.13
min
;
777
(bp) ,
258
(aa)
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Product
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imidazole glycerol phosphate synthase, catalytic subunit with HisH
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Operon Name
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hisLGDCBHAFI
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Note
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imidazole glycerol phosphate synthase subunit in heterodimer with HisH = imidazole glycerol phsphate synthase holoenzyme; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0000105 - histidine biosynthetic process
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Function
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enzyme; Amino acid biosynthesis: Histidine
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Gene Ontology
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GO:0000105
;
histidine biosynthetic process ( hisF )
GO:0000107
;
imidazoleglycerol-phosphate synthase activity ( hisF )
GO:0003824
;
catalytic activity ( hisF )
GO:0005737
;
cytoplasm ( hisF )
GO:0008152
;
metabolic process ( hisF )
GO:0008652
;
amino acid biosynthetic process ( hisF )
GO:0009382
;
imidazoleglycerol-phosphate synthase complex ( hisF )
GO:0016829
;
lyase activity ( hisF )
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PID
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1788336
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EC number
(KEGG Pathway)
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2.4.2.-
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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HIS6_ECOLI
(
P60664
;
P10373
)
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- Protein name
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Imidazole glycerol phosphate synthase subunit hisF
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- Synonyms
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EC 4.1.3.-, IGP synthase cyclase subunit, IGP synthase subunit hisF, ImGP synthase subunit hisF, IGPS subunit hisF
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- Gene name
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Name=hisF; OrderedLocusNames=b2025;
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- Comments
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FUNCTION:IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit.
CATALYTIC ACTIVITY:5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H(2)O.
PATHWAY:Histidine biosynthesis; fifth step.
SUBUNIT:Heterodimer of hisH and hisF.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:Belongs to the hisA / hisF family.
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Entry name(Acc.no)
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HIS6_ECO57
(
P60665
;
P10373
)
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- Protein name
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Imidazole glycerol phosphate synthase subunit hisF
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- Synonyms
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EC 4.1.3.-, IGP synthase cyclase subunit, IGP synthase subunit hisF, ImGP synthase subunit hisF, IGPS subunit hisF
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- Gene name
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Name=hisF; OrderedLocusNames=z3187, ECs2826;
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- Comments
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FUNCTION:IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit (By similarity).
CATALYTIC ACTIVITY:5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H(2)O.
PATHWAY:Histidine biosynthesis; fifth step.
SUBUNIT:Heterodimer of hisH and hisF (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the hisA / hisF family.
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Entry name(Acc.no)
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HIS6_ECOL6
(
Q8FG48
)
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- Protein name
|
Imidazole glycerol phosphate synthase subunit hisF
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- Synonyms
|
EC 4.1.3.-, IGP synthase cyclase subunit, IGP synthase subunit hisF, ImGP synthase subunit hisF, IGPS subunit hisF
|
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- Gene name
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Name=hisF; OrderedLocusNames=c2552;
|
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- Comments
|
FUNCTION:IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit (By similarity).
CATALYTIC ACTIVITY:5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H(2)O.
PATHWAY:Histidine biosynthesis; fifth step.
SUBUNIT:Heterodimer of hisH and hisF (By similarity).
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the hisA / hisF family.
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MMBR References
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J Mol Evol. 1994;38(5):489-95.
The evolution of the histidine biosynthetic genes in prokaryotes: a common ancestor for the hisA and hisF genes.
Fani R, Lio P, Chiarelli I, Bazzicalupo M.
(
8028028
)
|
J Bacteriol. 1995;177:4841-4850
Suppression of the pleiotropic effects of HisH and HisF overproduction identifies four novel loci on the Salmonella typhimurium chromosome: osmH, sfiW, sfiX, and sfiY.
Flores, A., and J. Casadesus.
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Mol Gen Genet. 1993;240(3):348-54.
Excess histidine enzymes cause AICAR-independent filamentation in Escherichia coli.
Frandsen N, D'Ari R.
(
8413183
)
|
Genetics 1970;66:231-244
Histidine-requiring mutants of Escherichia coli K12.
Garrick-Silversmith, L., and P. E. Hartman.
|
Genetics 1970;66:219-229
Genetic analysis of the histidine operon in Escherichia coli K12.
Goldschmidt, E. P., M. S. Cater, T. S. Matney, M. A. Butler, and A. Greene.
|
Biochemistry. 1993;32(19):5177-86.
Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis.
Klem TJ, Davisson VJ.
(
8494895
)
|
J Bacteriol. 1992;174(11):3830-1.
Location of the hisGDCBHAFI operon on the physical map of Escherichia coli.
Alifano P, Carlomagno MS, Bruni CB.
(
1592835
)
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| Sequences |
Amino acid
FASTA format
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0001 MLAKRIIPCL DVRDGQVVKG VQFRNHEIIG DIVPLAKRYA EEGADELVFY DITASSDGRV VDKSWVSRVA
0071 EVIDIPFCVA GGIKSLEDAA KILSFGADKI SINSPALADP TLITRLADRF GVQCIVVGID TWYDAETGKY
0141 HVNQYTGDES RTRVTQWETL DWVQEVQKRG AGEIVLNMMN QDGVRNGYDL EQLKKVREVC HVPLIASGGA
0211 GTMEHFLEAF RDADVDGALA ASVFHKQIIN IGELKAYLAT QGVEIRIC
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACATTGATGA TGTGGCGGCC CTGCGTGGCA
-070 CTGGTGTGCG CGGCGTAATA GTTGGTCGGG CATTACTGGA AGGTAAATTC ACCGTGAAGG AGGCCATCGC
0001 atgctggcaa aacgcataat cccatgtctc gacgttcgtg atggtcaggt ggtgaaaggc gtacagtttc
0071 gcaaccatga aatcattggc gatatcgtgc cgctggcaaa acgctacgct gaagaaggcg ctgacgaact
0141 ggtgttctac gatatcaccg cttccagcga tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg
0211 gaagtgatcg acattccgtt ttgtgtggcg ggtgggatta agtctctgga agatgccgcg aaaattcttt
0281 cctttggcgc ggataaaatt tccatcaact ctcctgcgct ggcagaccca acattaatta ctcgcctggc
0351 cgatcgcttt ggcgtgcagt gtattgtggt cggtattgat acctggtacg acgccgaaac cggtaaatat
0421 catgtgaatc aatataccgg cgatgaaagc cgcacccgcg tcactcaatg ggaaacgctc gactgggtac
0491 aggaagtgca aaaacgcggt gccggagaaa tcgtcctcaa tatgatgaat caggacggcg tgcgtaacgg
0561 ttacgacctc gaacaactga aaaaagtgcg tgaagtttgc cacgtcccgc tgattgcctc cggtggcgcg
0631 ggcaccatgg aacacttcct cgaagccttc cgcgatgccg acgttgacgg cgcgctggca gcttccgtat
0701 tccacaaaca aataatcaat attggtgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat
0771 atgttaaCAG AACAACAACG TCGCGAACTG GACTGGGAAA AAACCGACGG ACTTATGCCG GTGATTGTGC
0841 AACACGCGGT ATCCGGCGAA GTGCTAATGC TGGGCTA
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