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PEC Original Annotations
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|
Essentiality
|
|
Class
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non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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|
|
Deletion
|
OCR36
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
rcsF
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
rcsF
Search strains by all related name:
rcsF b0196 ECK0196 JW0192
|
General information (Go to Linear View: )
|
|
Gene Name
|
rcsF
|
|
Alternative name
|
b0196,ECK0196,JW0192
|
|
Location, Length
|
219,591 - 219,995
(
-
)
;
4.73
min
;
405
(bp) ,
134
(aa)
|
|
Product
|
putative outer membrane protein, signal
|
|
Operon Name
|
rcsF-yaeB
|
|
Note
|
regulator in colanic acid synthesis; interacts with RcsB; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009242 - colanic acid biosynthetic process
|
|
Function
|
regulator; Surface polysaccharides & antigens
|
|
Gene Ontology
|
GO:0009279
;
cell outer membrane ( rcsF )
GO:0016020
;
membrane ( rcsF )
|
|
PID
|
1786394
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
RCSF_ECOLI
(
P28633
)
|
|
- Protein name
|
Protein rcsF.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=rcsF; OrderedLocusNames=b0196, c0237;
|
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- Comments
|
FUNCTION:Stimulates colanic acid capsule synthesis. May promote the phosphorylation of rcsB.
|
|
Entry name(Acc.no)
|
Q8X8W0
(
Q8X8W0
)
|
|
- Protein name
|
Regulator in colanic acid synthesis; interacts with RcsB.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=rcsF; OrderedLocusNames=z0208;
|
|
- Comments
|
CATALYTIC ACTIVITY:Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.
SIMILARITY:Belongs to family 24 of glycosyl hydrolases.
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
rcsF
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Protein rcsF
|
|
PSI-BLAST
|
Protein rcsF
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
hypothetical protein EcolF_01001449 [Escherichia coli F11]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
|
|
MMBR References
|
J Bacteriol. 1992;174:8016-8022
Identification, cloning, and characterization of rcsF, a new regulator gene for exopolysaccharide synthesis that suppresses the division mutation ftsZ84 in Escherichia coli K-12.
Gervais, F. G., and G. R. Drapeau.
|
| Sequences |
Amino acid
FASTA format
|
0001 MRALPICLVA LMLSGCSMLS RSPVEPVQST APQPKAEPAK PKAPRATPVR IYTNAEELVG KPFRDLGEVS
0071 GDSCQASNQD SPPSIPTARK RMQINASKMK ANAVLLHSCE VTSGTPGCYR QAVCIGSALN ITAK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 caatttgtaa tatcattcag gacgggcgct
-070 tgcccgtctt gtcattttta caagctcctg attcaatatt gacgttttga tcatacattg aggaaatact
0001 atgcgtgctt taccgatctg tttagtagca ctcatgctaa gcggctgttc catgttaagc agatcccctg
0071 tcgaacccgt tcaaagcact gcaccccagc cgaaagcgga gcctgcaaaa ccgaaagcgc cgcgcgccac
0141 gccggtccga atttatacca atgcagaaga attagtcggc aaaccgttcc gcgatctcgg tgaagtcagt
0211 ggcgactctt gccaggcctc taatcaggac tctccgccga gcattccaac cgcacgtaag cggatgcaaa
0281 tcaacgcctc taaaatgaaa gccaatgctg tattactgca tagctgcgaa gtcaccagcg gtacgccagg
0351 ctgctatcgt caggctgtat gtatcggttc tgcgcttaac attacggcga aatgagcagt ttccagttcg
0421 agcaaatagg cgttattcgc tcgccctata aagaaaagtt cgccgttccg cgccagccag gtctggtaaa
0491 aagcgccaac ggcga
|
