MG1655
W3110
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Gene Report : argS
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
     Deletion OCL79,81-3 (BK5-5 / AK4-5) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  argS
Related strains
     Strains Search Search strains by gene name:  argS   Search strains by all related name:  argS b1876 ECK1877 JW1865 lov

General information  (Go to Linear View:)
 Gene Name argS  
 Alternative name b1876,ECK1877,JW1865,lov  
 Location, Length 1,958,086 - 1,959,819 (  +  ) ;   42.20 min ; 1734 (bp) ,   577 (aa) Go to Linear View
 Product arginyl-tRNA synthetase  
 Operon Name argS  
 Note arginine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation  
 Function enzyme; Aminoacyl tRNA synthetases, tRNA modification  
 Gene Ontology GO:0000166; nucleotide binding ( argS )
GO:0004812; aminoacyl-tRNA ligase activity ( argS )
GO:0004814; arginine-tRNA ligase activity ( argS )
GO:0005524; ATP binding ( argS )
GO:0005737; cytoplasm ( argS )
GO:0006412; translation ( argS )
GO:0006418; tRNA aminoacylation for protein translation ( argS )
GO:0006420; arginyl-tRNA aminoacylation ( argS )
GO:0016874; ligase activity ( argS )
 PID 1788184  
 EC number
  (KEGG Pathway)
 
6.1.1.19  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene argS ( EG10071 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) SYR_ECOLI ( P11875 )
    -  Protein name Arginyl-tRNA synthetase  
    -  Synonyms EC 6.1.1.19, Arginine--tRNA ligase, ArgRS  
    -  Gene name Name=argS; OrderedLocusNames=b1876;  
    -  Comments
  • CATALYTIC ACTIVITY:ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
  • SUBUNIT:Monomer.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Belongs to class-I aminoacyl-tRNA synthetase family.  
  •   botton Entry name(Acc.no) SYR_ECOL6 ( Q8FGQ0 )
        -  Protein name Arginyl-tRNA synthetase  
        -  Synonyms EC 6.1.1.19, Arginine--tRNA ligase, ArgRS  
        -  Gene name Name=argS; OrderedLocusNames=c2291;  
        -  Comments
  • CATALYTIC ACTIVITY:ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
  • SUBUNIT:Monomer (By similarity).
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Belongs to class-I aminoacyl-tRNA synthetase family.  
  •   botton Entry name(Acc.no) SYR_ECO57 ( Q8XCH2 )
        -  Protein name Arginyl-tRNA synthetase  
        -  Synonyms EC 6.1.1.19, Arginine--tRNA ligase, ArgRS  
        -  Gene name Name=argS; OrderedLocusNames=z2929, ECs2586;  
        -  Comments
  • CATALYTIC ACTIVITY:ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
  • SUBUNIT:Monomer (By similarity).
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Belongs to class-I aminoacyl-tRNA synthetase family.  

  •  Linear View (Whole Mode)
    View Location
       1945.0  –  1970.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP argS (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Yeast Arginyl-Trna Synthetase  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Arginyl-tRNA synthetase  
             PSI-BLAST Arginyl-tRNA synthetase
        nr     (database updated : 2007.02.20 )
             BLAST arginyl-tRNA synthetase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AA_TRNA_LIGASE_I     

    Other Cross-Reference
        COG COG0018J 
        EcoCyc argS 

    MMBR References
    Nucleic Acids Res. 1989;17:5725-5736
    Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase.
    Eriani, G., G. Dirheimer, and J. Gangloff.
    J Mol Biol. 1968;35(1):83-93.
    Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. 3. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants.
    Hirshfield IN, Horn PC, Hopwood DA, Maas WK, DeDeken R. ( 4939781 )
    Microbiol. Rev. 1983;47:231-284.
    Gene-protein index of Escherichia coli K-12.
    Neidhardt, F. C., V. Vaughn, T. A. Phillips, and P. L. Bloch.
    EMBO J. 1992;11:1493-1501
    Penicillin binding protein 2 is dispensable in Escherichia coli when ppGpp synthesis is induced.
    Vinella, D., R. D'Ari, and P. Bouloc.

    Sequences
    Amino acid
    FASTA format
    0001 MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK LGMAPRQLAE QVLTHLDLNG 
    0071 IASKVEIAGP GFINIFLDPA FLAEHVQQAL ASDRLGVATP EKQTIVVDYS APNVAKEMHV GHLRSTIIGD 
    0141 AAVRTLEFLG HKVIRANHVG DWGTQFGMLI AWLEKQQQEN AGEMELADLE GFYRDAKKHY DEDEEFAERA 
    0211 RNYVVKLQSG DEYFREMWRK LVDITMTQNQ ITYDRLNVTL TRDDVMGESL YNPMLPGIVA DLKAKGLAVE 
    0281 SEGATVVFLD EFKNKEGEPM GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA 
    0351 IVRKAGYVPE SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER ARRLVAEKNP DMPADELEKL 
    0421 ANAVGIGAVK YADLSKNRTT DYIFDWDNML AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ LAAAPVIIRE 
    0491 DREAQLAARL LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL SAENEEVRNS RLKLAQLTAK 
    0561 TLKLGLDTLG IETVERM
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             ACGTTGCTGA CGAAAACAGC CATTTGCAGT 
    -070 ATACTCCCGC CCTAATTTCT TTAACTGGTG CGGGCAATTT TTGCTCGCTT CATCAATGTA AGGTATTCCG 
    0001 gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt 
    0071 gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca tgatggcagt 
    0141 tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga ctcatctgga tcttaacggt 
    0211 atcgccagca aagttgagat cgccggtcca ggctttatca acattttcct tgatccggca ttcctggctg 
    0281 aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt 
    0351 tgactactct gcgccaaacg tggcgaaaga gatgcatgtc ggtcacctgc gctctaccat tattggtgac 
    0421 gcagcagtgc gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca 
    0491 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa tggagctggc 
    0561 tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gatgaagatg aagagttcgc cgagcgcgca 
    0631 cgtaactacg tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca 
    0701 tcaccatgac gcagaaccag atcacctacg atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg 
    0771 cgaaagcctc tacaacccga tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa 
    0841 agcgaagggg cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca 
    0911 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc gttatgaaac 
    0981 actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac acctgatgca ggcatgggcg 
    1051 atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg 
    1121 gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc tggatgaagc 
    1191 cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag ccgacgagct ggaaaaactg 
    1261 gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct 
    1331 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgcat acacgcgtgt 
    1401 attgtccgtg ttccgtaaag cagaaattga cgaagagcaa ctggctgcag ctccggttat catccgtgaa 
    1471 gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag 
    1541 gcacgccgca tgtaatgtgt gcttacctgt acgatctggc cggtctgttc tctggcttct acgagcactg 
    1611 cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctaaaac tggcacaact gacggcgaag 
    1681 acgctgaagc tgggtctgga tacgctgggt attgagactg tagagcgtat gtaaTCGATT TTTCGTGAGA 
    1751 GTGAAGCCTG ATCAGGGTTA GCCGATCAGG CTTTTTTATT GCCATCTAAA TGTATTCTGA AAATGGACAT 
    1821 GCCATTGTTT TCTC