MG1655
W3110
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Gene Report : manY
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL82 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  manY
Related strains
     Strains Search Search strains by gene name:  manY   Search strains by all related name:  manY b1818 ECK1816 JW1807 pel ptsM ptsP ptsX

General information  (Go to Linear View:)
 Gene Name manY  
 Alternative name b1818,ECK1816,JW1807,pel,ptsM,ptsP,ptsX  
 Location, Length 1,901,106 - 1,901,906 (  +  ) ;   40.97 min ; 801 (bp) ,   266 (aa) Go to Linear View
 Product mannose-specific enzyme IIC component of PTS  
 Operon Name manXYZ  
 Note PTS enzyme IIC, mannose-specific; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0003824 ; catalytic activity ( ptsP )
GO:0005351 ; sugar:hydrogen ion symporter activity ( manY )
GO:0005351 ; sugar:hydrogen ion symporter activity ( ptsP )
GO:0005737 ; cytoplasm ( ptsP )
GO:0005886 ; plasma membrane ( manY )
GO:0006810 ; transport ( manY )
GO:0006810 ; transport ( ptsP )
GO:0008643 ; carbohydrate transport ( manY )
GO:0008643 ; carbohydrate transport ( ptsP )
GO:0008965 ; phosphoenolpyruvate-protein phosphotransferase activity ( ptsP )
GO:0009401 ; phosphoenolpyruvate-dependent sugar phosphotransferase system ( manY )
GO:0009401 ; phosphoenolpyruvate-dependent sugar phosphotransferase system ( ptsP )
GO:0016020 ; membrane ( manY )
GO:0016021 ; integral to membrane ( manY )
GO:0016301 ; kinase activity ( ptsP )
GO:0016310 ; phosphorylation ( ptsP )
GO:0016740 ; transferase activity ( ptsP )
GO:0016772 ; transferase activity, transferring phosphorus-containing groups ( ptsP )
 PID 1788121  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) PTNC_ECOLI ( P08187 ; Q47351 )
    -  Protein name PTS system, mannose-specific IIC component  
    -  Synonyms EIIC-Man, Mannose- permease IIC component, Phosphotransferase enzyme II, C component, EII-P-Man  
    -  Gene name OrderedLocusNames=b1818, c2224, z2861, ECs2528, SF1410, S1525;  
    -  Comments
  • FUNCTION:This is a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system. The IICD domains contain the sugar binding site and the transmembrane channel; the IIA domain contains the primary phosphorylation site (the donor is phospho-HPr); IIA transfers its phosphoryl group to the IIB domain which finally transfers it to the sugar.
  • SUBCELLULAR LOCATION:Integral membrane protein.
  • SIMILARITY:Contains 1 PTS EIIC domain.  
    •   botton Entry name(Acc.no) PTND_ECOLI ( P08188 )
        -  Protein name PTS system, mannose-specific IID component  
        -  Synonyms EIID-Man, Mannose- permease IID component, Phosphotransferase enzyme II, D component, EII-M-Man  
        -  Gene name OrderedLocusNames=b1819, c2226, z2862, ECs2529;  
        -  Comments
  • FUNCTION:This is a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system. The IICD domains contain the sugar binding site and the transmembrane channel; the IIA domain contains the primary phosphorylation site (the donor is phospho-HPr); IIA transfers its phosphoryl group to the IIB domain which finally transfers it to the sugar.
  • SUBCELLULAR LOCATION:Integral membrane protein.
  • SIMILARITY:Contains 1 PTS EIID domain.  
    •   botton Entry name(Acc.no) PT1P_ECOLI ( P37177 )
        -  Protein name Phosphoenolpyruvate-protein phosphotransferase ptsP  
        -  Synonyms EC 2.7.3.9, Phosphotransferase system, enzyme I, Enzyme I-Ntr  
        -  Gene name Name=ptsP; OrderedLocusNames=b2829;  
        -  Comments
  • FUNCTION:Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (ptsO) and IIANtr (ptsN) proteins thereby providing a link between carbon and nitrogen assimilatory pathways.
  • CATALYTIC ACTIVITY:Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.
  • SUBCELLULAR LOCATION:Cytoplasmic (Probable).
  • SIMILARITY:Belongs to the PEP-utilizing enzyme family.
  • SIMILARITY:Contains 1 GAF domain.  
    •   botton Entry name(Acc.no) Q8FEB0 ( Q8FEB0 )
        -  Protein name Phosphoenolpyruvate-protein phosphotransferase ptsP  
        -  Synonyms EC 2.7.3.9  
        -  Gene name Name=ptsP; OrderedLocusNames=c3424;  
        -  Comments
  • SIMILARITY:Belongs to the ATP-dependent AMP-binding enzyme family.  
    •   botton Entry name(Acc.no) Q8X6K3 ( Q8X6K3 )
        -  Protein name PTS system, enzyme I, transcriptional regulator  
        -  Synonyms With NPR and NTR proteins  
        -  Gene name Name=ptsP; OrderedLocusNames=z4146;  
        -  Comments
  • SIMILARITY:Belongs to the ATP-dependent AMP-binding enzyme family.  

    •  Linear View (Whole Mode)
    View Location
       1890.0  –  1915.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP manY (  homologous genes of other bacterias  )   ptsP (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
            PSI-BLAST  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Mannose permease IIC component (PTS system mannose-specific EIIC component) (EIIC-Man) (EII-P-Man)  
             PSI-BLAST Mannose permease IIC component (PTS system mannose-specific EIIC component) (EIIC-Man) (EII-P-Man)
        nr     (database updated : 2007.02.20 )
             BLAST PTS enzyme IIC, mannose-specific [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN     

    Other Cross-Reference
        EcoCyc manY 

    MMBR References
    J Biol Chem. 1985;260:15495-15503
    The mannose-permease of the bacterial phosphotransferase system. Gene cloning and purification of the enzyme IIMan/IIIMan complex of Escherichia coli.
    Erni, B., and B. Zanolari.
    J Biol Chem. 1987;262:5238-5247
    The mannose permease of Escherichia coli consists of three different proteins: amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA.
    Erni, B., B. Zanolari, and H. P. Kocher.
    Eur J Biochem. 1996;239:810-817
    Membrane topology of the mannose transporter of Escherichia coli K12.
    Huber, F., and B. Erni.
    Mol Gen Genet. 1985;199(3):427-33.
    Gene fusions to the ptsM/pel locus of Escherichia coli.
    Palva ET, Saris P, Silhavy TJ. ( 3162078 )
    Proc Natl Acad Sci U S A. 1986;83:8934-8938
    Pel, the protein that permits lambda DNA penetration of Escherichia coli, is encoded by a gene in ptsM and is required for mannose utilization by the phosphotransferase system.
    Williams, N., D. K. Fox, C. Shea, and S. Roseman.

    Sequences
    Amino acid
    FASTA format 
    0001 MEITTLQIVL VFIVACIAGM GSILDEFQFH RPLIACTLVG IVLGDMKTGI IIGGTLEMIA LGWMNIGAAV 
0071 APDAALASII STILVIAGHQ SIGAGIALAI PLAAAGQVLT IIVRTITVAF QHAADKAADN GNLTAISWIH 
0141 VSSLFLQAMR VAIPAVIVAL SVGTSEVQNM LNAIPEVVTN GLNIAGGMIV VVGYAMVINM MRAGYLMPFF 
0211 YLGFVTAAFT NFNLVALGVI GTVMAVLYIQ LSPKYNRVAG APAQAAGNND LDNELD
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             TGAAAATGAT GGATCTGATC AGCAAAATCG 
-070 ATAAGTAACG TATTGTGTTG ATTATCACTC AGTTTTCACA CTTAAGTCTT ACGTAAACAG GAGAAGTACA 
0001 atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat cgcaggtatg ggatcaatcc 
0071 tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac cctggtgggt atcgttcttg gggatatgaa 
0141 aaccggtatt attatcggtg gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt 
0211 gcgcctgacg ccgctctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg 
0281 caggtatcgc actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac 
0351 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc ctggatccac 
0421 gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat cgttgcgctg tctgttggta 
0491 ccagcgaagt acagaacatg ctgaatgcga ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg 
0561 catgatcgtg gtggttggtt atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc 
0631 tacctcggct tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta 
0701 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggcagctgg 
0771 taacaacgat ctcgataacg aactggacta aCAGGTGAGC GAAATGGTTG ATACAACTCA AACTACCACC 
0841 GAGAAAAAAC TCACTCAAAG TGATATTCGT GGCGTCTTCC TGCGTTCTAA CCTCTTCCAG G