MG1655
W3110
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Gene Report : tsf
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)  
     Deletion OCR33,34-1 (D)  ,  OCR33,34-2 (D)  ,  OCR33,35-4 (AK4-2 / BK5-2) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  tsf
Related strains
     Strains Search Search strains by gene name:  tsf   Search strains by all related name:  tsf b0170 ECK0169 JW0165

General information  (Go to Linear View:)
 Gene Name tsf  
 Alternative name b0170,ECK0169,JW0165  
 Location, Length 190,857 - 191,708 (  +  ) ;   4.11 min ; 852 (bp) ,   283 (aa) Go to Linear View
 Product protein chain elongation factor EF-Ts  
 Operon Name tff-rpsB-tsf  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation  
 Function factor; Proteins - translation and modification  
 Gene Ontology GO:0003746; translation elongation factor activity ( tsf )
GO:0005515; protein binding ( tsf )
GO:0005622; intracellular ( tsf )
GO:0005624; membrane fraction ( tsf )
GO:0005737; cytoplasm ( tsf )
GO:0006412; translation ( tsf )
GO:0006414; translational elongation ( tsf )
 PID 1786366  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene tsf ( EG11033 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) EFTS_ECOLI ( P02997 )
    -  Protein name Elongation factor Ts  
    -  Synonyms EF-Ts  
    -  Gene name OrderedLocusNames=b0170, c0206, z0181, ECs0172, SF0160, S0163;  
    -  Comments
  • FUNCTION:Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
  • SUBUNIT:Heterotetramer composed of two EF-Ts.EF-Tu dimer complex.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • MASS SPECTROMETRY:MW=30294; MW_ERR=6; METHOD=Electrospray; RANGE=1-282; NOTE=Ref.14.
  • SIMILARITY:Belongs to the EF-Ts family.  

  •  Linear View (Whole Mode)
    View Location
       180.0  –  205.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP tsf (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain B, Elongation Factor Complex Ef-TuEF-Ts From Escherichia Coli  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Elongation factor Ts (EF-Ts)  
             PSI-BLAST Elongation factor Ts (EF-Ts)
        nr     (database updated : 2007.02.20 )
             BLAST elongation factor Ts [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    EF_TS_1    EF_TS_2     

    Other Cross-Reference
        EcoCyc tsf 

    MMBR References
    Mol Gen Genet. 1981;182(1):178-9.
    Revised location of the Escherichia coli gene coding for ribosomal protein S2.
    Lathe R, Bollen A, Herzog R. ( 7022135 )
    Mol Gen Genet. 1981;181(3):356-62.
    Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed.
    Bendiak DS, Friesen JD. ( 7017348 )
    J Mol Biol. 1979;132(2):219-33.
    A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB).
    Bollen A, Lathe R, Herzog A, Denicourt D, Lecocq JP, Desmarez L, Lavalle R. ( 161328 )
    Nucleic Acids Res. 1981;9:4163-4172
    Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.
    An, G., D. S. Bendiak, L. A. Mamelak, and J. D. Friesen.

    Sequences
    Amino acid
    FASTA format
    0001 MAEITASLVK ELRERTGAGM MDCKKALTEA NGDIELAIEN MRKSGAIKAA KKAGNVAADG VIKTKIDGNY 
    0071 GIILEVNCQT DFVAKDAGFQ AFADKVLDAA VAGKITDVEV LKAQFEEERV ALVAKIGENI NIRRVAALEG 
    0141 DVLGSYQHGA RIGVLVAAKG ADEELVKHIA MHVAASKPEF IKPEDVSAEV VEKEYQVQLD IAMQSGKPKE 
    0211 IAEKMVEGRM KKFTGEVSLT GQPFVMEPSK TVGQLLKEHN AEVTGFIRFE VGEGIEKVET DFAAEVAAMS 
    0281 KQS
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             TAGTACACGT TTGGTTAGGG GGCCTGCATA 
    -070 TGGCCCCCTT TTTCACTTTT ATATCTGTGC GGTTTAATGC CGGGCAGATC ACATCTCCGA GGATTTTAGA 
    0001 atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg atggattgca 
    0071 aaaaagcact gactgaagct aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat 
    0141 taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac 
    0211 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag 
    0281 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga 
    0351 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc gctggaaggc 
    0421 gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag 
    0491 agctggttaa acacatcgct atgcacgttg ctgcaagcaa gccagaattc atcaaaccgg aagacgtatc 
    0561 cgctgaagtg gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa 
    0631 atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt 
    0701 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat 
    0771 ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc tgcgatgtcc 
    0841 aagcagtctt aaTTATCAAA AAGGAGCCGC CTGAGGGCGG CTTCTTTTTG TGCCCATCTT GTAAATTCAG 
    0911 CTAACCCTTG TGGGGCTGCG CTGAAAAGCG ACGTACAATG TC