MG1655
W3110
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Gene Report : hdhA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(PLK1608)  ,  OCL90-3 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  hdhA
Related strains
     Strains Search Search strains by gene name:  hdhA   Search strains by all related name:  hdhA b1619 ECK1614 hsd hsdH JW1611

General information  (Go to Linear View:)
 Gene Name hdhA  
 Alternative name b1619,ECK1614,hsd,hsdH,JW1611  
 Location, Length 1,695,297 - 1,696,064 (  -  ) ;   36.54 min ; 768 (bp) ,   255 (aa) Go to Linear View
 Product 7-alpha-hydroxysteroid dehydrogenase, NAD-dependent  
 Operon Name hdhA  
 Note NAD-dependent 7alpha-hydroxysteroid dehydrogenase, dehydroxylation of bile acids; GO_component: GO:0005737 - cytoplasm  
 Function enzyme; Central intermediary metabolism: Pool, multipurpose conversions  
 Gene Ontology GO:0003824 ; catalytic activity ( hdhA )
GO:0005488 ; binding ( hdhA )
GO:0006629 ; lipid metabolic process ( hdhA )
GO:0008152 ; metabolic process ( hdhA )
GO:0008202 ; steroid metabolic process ( hdhA )
GO:0008709 ; 7-alpha-hydroxysteroid dehydrogenase activity ( hdhA )
GO:0016491 ; oxidoreductase activity ( hdhA )
GO:0030573 ; bile acid catabolic process ( hdhA )
 PID 1787905  
 EC number
  (KEGG Pathway)   		1.1.1.159  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene hdhA ( EG10425 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) HDHA_ECOLI ( P25529 )
    -  Protein name 7-alpha-hydroxysteroid dehydrogenase  
    -  Synonyms EC 1.1.1.159, 7-alpha-HSDH  
    -  Gene name Name=hdhA; Synonyms=hsdH; OrderedLocusNames=b1619, z2624, ECs2327;  
    -  Comments
  • FUNCTION:7-alpha-dehydroxylation of cholic acid, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid.
  • CATALYTIC ACTIVITY:3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH.
  • SUBUNIT:Homotetramer.
  • SIMILARITY:Belongs to the short-chain dehydrogenases/reductases (SDR) family.  
    •   botton Entry name(Acc.no) Q8FHA2 ( Q8FHA2 )
        -  Protein name 7-alpha-hydroxysteroid dehydrogenase  
        -  Synonyms EC 1.1.1.159  
        -  Gene name Name=hdhA; OrderedLocusNames=c2011;  
        -  Comments
  • SIMILARITY:Belongs to the short-chain dehydrogenases/reductases (SDR) family.  

    •  Linear View (Whole Mode)
    View Location
       1685.0  –  1710.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP hdhA (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of Orf 1438 A Putative GlucoseRIBITOL Dehydrogenase From Clostridium Thermocellum  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST 7-alpha-hydroxysteroid dehydrogenase (7-alpha-HSDH)  
             PSI-BLAST 3-oxoacyl-[acyl-carrier-protein] reductase
        nr     (database updated : 2007.02.20 )
             BLAST 7-alpha-hydroxysteroid dehydrogenase [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ADH_SHORT     

    Other Cross-Reference
        COG COG1028IQR 
        EcoCyc hdhA 

    MMBR References
    J Bacteriol. 1993;175(17):5730
    Location of the 7 alpha-hydroxysteroid dehydrogenase gene (hdhA) on the physical map of the Escherichia coli chromosome.
    Yoshimoto T, Nagai H, Ito K, Tsuru D. ( 8366061 )

    Sequences
    Amino acid
    FASTA format 
    0001 MFNSDNLRLD GKCAIITGAG AGIGKEIAIT FATAGASVVV SDINADAANH VVDEIQQLGG QAFACRCDIT 
0071 SEQELSALAD FAISKLGKVD ILVNNAGGGG PKPFDMPMAD FRRAYELNVF SFFHLSQLVA PEMEKNGGGV 
0141 ILTITSMAAE NKNINMTSYA SSKAAASHLV RNMAFDLGEK NIRVNGIAPG AILTDALKSV ITPEIEQKML 
0211 QHTPIRRLGQ PQDIANAALF LCSPAASWVS GQILTVSGGG VQELN
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             tctgctatac ctggtagtgt cctttcctca 
-070 aggttaatga taaaaaatgt ggcacacagg cggtgccttt tatttttact gaagttcaca ggaggtttat 
0001 gtgtttaatt ctgacaacct gagactcgac ggaaaatgcg ccatcatcac aggtgcgggt gcaggtattg 
0071 gtaaagaaat cgccattaca ttcgcgacag ctggcgcatc tgtggtggtc agtgatatta acgccgacgc 
0141 agctaaccat gttgtagacg aaattcaaca actgggtggt caggcatttg cctgccgttg tgatattact 
0211 tccgaacagg aactctctgc actggcagac tttgctatca gtaagctggg taaagttgat attctggtta 
0281 acaacgccgg tggcggtgga cctaaaccgt ttgatatgcc aatggcggat tttcgccgtg cttatgaact 
0351 gaatgtgttt tcttttttcc atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg cggtggcgtt 
0421 attctgacca tcacttctat ggcggcagaa aataaaaata taaacatgac ttcctatgca tcatctaaag 
0491 ctgcggccag tcatctggtc agaaatatgg cgtttgacct gggtgaaaaa aatattcggg taaatggcat 
0561 tgcgccgggg gcaatattaa ccgatgccct gaaatccgtt attacaccag aaattgaaca aaaaatgtta 
0631 cagcacacgc cgatcagacg tctgggccaa ccgcaagata ttgctaacgc agcgctgttc ctttgctcgc 
0701 ctgctgcgag ctgggtaagc ggacaaattc tcaccgtctc cggtggtggg gtacaggagc tcaattaata 
0771 cactaacgga ccggtaaaca accgtgcgtg ttgtttaccg ggataaactc atcaacgtct ctgctaaata 
0841 actggcagcc aaatcacggc tattggtt