MG1655
W3110
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Gene Report : tam
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 20 (LD)  ,  DE(PLK1608)  ,  OCR70,L90-6 (LD 20-1_P/O & 20-2TC) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  tam
Related strains
     Strains Search Search strains by gene name:  tam   Search strains by all related name:  tam b1519 ECK1512 JW1512 lsrE o252 o252a yneD

General information  (Go to Linear View:)
 Gene Name tam  
 Alternative name b1519,ECK1512,JW1512,lsrE,o252,o252a,yneD  
 Location, Length 1,605,370 - 1,606,128 (  +  ) ;   34.60 min ; 759 (bp) ,   252 (aa) Go to Linear View
 Product trans-aconitate methyltransferase  
 Operon Name lsrACDBFG-tam  
 Note trans-aconitate 2-methyltransferase; GO_component: GO:0005737 - cytoplasm  
 Function putative enzyme; Not classified  
 Gene Ontology GO:0005737 ; cytoplasm ( tam )
GO:0008152 ; metabolic process ( tam )
GO:0008168 ; methyltransferase activity ( tam )
GO:0016740 ; transferase activity ( tam )
GO:0030798 ; trans-aconitate 2-methyltransferase activity ( tam )
 PID 1787798  
 EC number
  (KEGG Pathway)   		2.1.1.144  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene tam ( EG13812 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) TAM_ECOLI ( P76145 )
    -  Protein name Trans-aconitate 2-methyltransferase  
    -  Synonyms EC 2.1.1.144  
    -  Gene name Name=tam; OrderedLocusNames=b1519;  
    -  Comments
  • FUNCTION:Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis-aconitate, isocitrate, and citrate at lower velocities and affinities.
  • CATALYTIC ACTIVITY:S-adenosyl-L-methionine + trans-aconitate = S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate.
  • SUBUNIT:Monomer.
  • SUBCELLULAR LOCATION:Cytoplasmic.
  • SIMILARITY:Belongs to the methyltransferase superfamily. Tam family.  
    •   botton Entry name(Acc.no) Q8FHF2 ( Q8FHF2 )
        -  Protein name Trans-aconitate 2-methyltransferase  
        -  Synonyms EC 2.1.1.144  
        -  Gene name Name=tam; OrderedLocusNames=c1942;  
        -  Comments
  • SIMILARITY:Contains 1 HTH marR-type DNA-binding domain.  
    •   botton Entry name(Acc.no) TAM_ECO57 ( Q8XAZ2 )
        -  Protein name Trans-aconitate 2-methyltransferase  
        -  Synonyms EC 2.1.1.144  
        -  Gene name Name=tam; OrderedLocusNames=z2186, ECs2126;  
        -  Comments
  • FUNCTION:Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate at high affinity and of cis-aconitate, isocitrate, and citrate at lower velocities and affinities (By similarity).
  • CATALYTIC ACTIVITY:S-adenosyl-L-methionine + trans-aconitate = S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate.
  • SUBUNIT:Monomer (By similarity).
  • SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
  • SIMILARITY:Belongs to the methyltransferase superfamily. Tam family.  

    •  Linear View (Whole Mode)
    View Location
       1595.0  –  1620.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP tam (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Crystal Structure Of The Putative Sam-Dependent Methyltransferase Bc2162 From Bacillus Cereus, Northeast Structural Genomics Target Bcr20.  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Trans-aconitate 2-methyltransferase  
             PSI-BLAST Dimethyladenosine transferase
        nr     (database updated : 2007.02.20 )
             BLAST trans-aconitate 2-methyltransferase [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        COG COG4106R 
        EcoCyc tam 

    Sequences
    Amino acid
    FASTA format 
    0001 MSDWNPSLYL HFSAERSRPA VELLARVPLE NVEYVADLGC GPGNSTALLQ QRWPAARITG IDSSPAMIAE 
0071 ARSALPDCQF VEADIRNWQP VQALDLIFAN ASLQWLPDHY ELFPHLVSLL NPQGVLAVQM PDNWLEPTHV 
0141 LMREVAWEQN YPDRGREPLA GVHAYYDILS EAGCEVDIWR TTYYHQMPSH QAIIDWVTAT GLRPWLQDLT 
0211 ESEQQLFLKR YHQMLEEQYP LQENGQILLA FPRLFIVARR ME
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             CCGGGCCGCG TAAAAAACGT CTGTTCAATG 
-070 GTTTGATGCC GTGAGGCGAA TTTATCAATT TTATCTACAA TTGGGGTAAC GCGCTGACGG GAGTAAAAAA 
0001 atgtctgact ggaacccctc tttatatcta cacttttccg ctgaacgatc gcgtccggcg gtggagctgc 
0071 ttgccagagt gccgctggaa aatgtcgaat atgttgccga tcttggctgt ggcccaggta acagcaccgc 
0141 ccttctacaa caacgttggc ctgcggccag gataacaggc atcgactcgt ctccggcgat gattgctgaa 
0211 gcgcgcagtg ctttgccaga ctgccagttc gtggaagcag acatccgcaa ctggcaaccg gtacaggcac 
0281 tcgatctgat ttttgctaat gcctcactgc aatggctgcc cgaccactac gaattgtttc ctcatctggt 
0351 ttctttactt aatccgcagg gcgtgctggc agtacagatg ccagataact ggctggagcc gacccatgtg 
0421 ctcatgcgcg aagttgcctg ggaacaaaac tacccggatc gcgggcgtga gccgttggct ggcgttcatg 
0491 cttactacga tattttgagc gaagccggat gtgaggtcga tatctggcga accacctact atcaccagat 
0561 gccgtcacac caggcgatta tcgattgggt gactgccact ggattacgtc cgtggttaca ggatctgacc 
0631 gagagcgaac agcagctttt tcttaagcgc taccatcaga tgctggaaga gcagtatcca ctgcaagaga 
0701 acggacagat actgctggca tttccgcgtc tgtttattgt tgcccggcgt atggagtaaA TATCACGTCA 
0771 GCTGGTAATG ACGATCGGGA AGAATTTTTG CTGGAATTTC GGCTTCATCG TTCATCTGTA ACAGGTCAAT 
0841 TTCAATAGCG TTGCAGATG