MG1655
W3110
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Gene Report : narY
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 20 (LD)  ,  DE(PLK1608)  ,  OCR70,L90-5 (LD 20-1_P/O) (D)  ,  OCR70,L90-6 (LD 20-1_P/O & 20-2TC) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  narY
Related strains
     Strains Search Search strains by gene name:  narY   Search strains by all related name:  narY b1467 chlZ ECK1461 JW1462

General information  (Go to Linear View:)
 Gene Name narY  
 Alternative name b1467,chlZ,ECK1461,JW1462  
 Location, Length 1,535,333 - 1,536,877 (  -  ) ;   33.09 min ; 1545 (bp) ,   514 (aa) Go to Linear View
 Product nitrate reductase 2 (NRZ), beta subunit  
 Operon Name narZYWV  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005506 ; iron ion binding ( narY )
GO:0005886 ; plasma membrane ( narY )
GO:0006118 ; electron transport ( narY )
GO:0006810 ; transport ( narY )
GO:0008940 ; nitrate reductase activity ( narY )
GO:0009055 ; electron carrier activity ( narY )
GO:0009325 ; nitrate reductase complex ( narY )
GO:0016020 ; membrane ( narY )
GO:0016491 ; oxidoreductase activity ( narY )
GO:0042126 ; nitrate metabolic process ( narY )
GO:0042128 ; nitrate assimilation ( narY )
GO:0046872 ; metal ion binding ( narY )
GO:0051536 ; iron-sulfur cluster binding ( narY )
GO:0051538 ; 3 iron, 4 sulfur cluster binding ( narY )
GO:0051539 ; 4 iron, 4 sulfur cluster binding ( narY )
 PID 1787740  
 EC number
  (KEGG Pathway)   		1.7.99.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene narY ( EG10647 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) NARY_ECOLI ( P19318 ; P78267 )
    -  Protein name Respiratory nitrate reductase 2 beta chain  
    -  Synonyms EC 1.7.99.4  
    -  Gene name Name=narY; OrderedLocusNames=b1467;  
    -  Comments
  • FUNCTION:This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.
  • CATALYTIC ACTIVITY:Nitrite + acceptor = nitrate + reduced acceptor.
  • COFACTOR:Binds 3 4Fe-4S clusters and 1 3Fe-4S cluster per subunit (By similarity).
  • SUBUNIT:Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.
  • SUBCELLULAR LOCATION:Membrane-associated.  
    •   botton Entry name(Acc.no) Q8FHI2 ( Q8FHI2 )
        -  Protein name Respiratory nitrate reductase 2 beta chain  
        -  Synonyms EC 1.7.99.4  
        -  Gene name Name=narY; OrderedLocusNames=c1899;  
        -  Comments
  • COFACTOR:Zinc (By similarity).
  • SIMILARITY:Belongs to the zinc-containing alcohol dehydrogenase family.  
    •   botton Entry name(Acc.no) Q8X9Y1 ( Q8X9Y1 )
        -  Protein name Cryptic nitrate reductase 2, beta subunit.  
        -  Synonyms  
        -  Gene name Name=narY; OrderedLocusNames=z2245;  
        -  Comments
  • SIMILARITY:Belongs to the arylamine N-acetyltransferase family.  

    •  Linear View (Whole Mode)
    View Location
       1525.0  –  1550.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP narY (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain B, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Respiratory nitrate reductase 2 beta chain  
             PSI-BLAST Tenascin-X precursor
        nr     (database updated : 2007.02.20 )
             BLAST nitrate reductase 2 (NRZ), beta subunit [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    CYTOCHROME_C     

    Other Cross-Reference
        COG COG1140C 
        EcoCyc narY 

    MMBR References
    Mol Gen Genet. 1990;222(1):104-11.
    Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.
    Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M. ( 2233673 )
    Mol Microbiol. 1992;6(2):221-30.
    Involvement of the narJ or narW gene product in the formation of active nitrate reductase in Escherichia coli.
    Blasco F, Pommier J, Augier V, Chippaux M, Giordano G. ( 1545706 )
    FEMS Microbiol Lett. 1997;147(1):147-9.
    Organization of the nar genes at the chlZ locus.
    Bonnefoy V, Ratouchniak J, Blasco F, Chippaux M. ( 9037773 )

    Sequences
    Amino acid
    FASTA format 
    0001 MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TGREGMEYAW FNNVETKPGI GYPKNWEDQE EWQGGWVRDV 
0071 NGKIRPRLGN KMGVITKIFA NPVVPQIDDY YEPFTFDYEH LHSAPEGKHI PTARPRSLID GKRMDKVIWG 
0141 PNWEELLGGE FEKRARDRNF EAMQKEMYGQ FENTFMMYLP RLCEHCLNPS CVATCPSGAI YKREEDGIVL 
0211 IDQDKCRGWR LCISGCPYKK IYFNWKSGKS EKCIFCYPRI ESGQPTVCSE TCVGRIRYLG VLLYDADRIE 
0281 EAASTEREVD LYERQCEVFL DPHDPSVIEE ALKQGIPQNV IDAAQRSPVY KMAMDWKLAL PLHPEYRTLP 
0351 MVWYVPPLSP IQSYADAGGL PKSEGVLPAI ESLRIPVQYL ANMLSAGDTG PVLRALKRMM AMRHYMRSQT 
0421 VEGVTDTRAI DEVGLSVAQV EEMYRYLAIA NYEDRFVIPT SHREMAGDAF AERNGCGFTF GDGCHGSDSK 
0491 FNLFNSSRID AINITEVRDK AEGE
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             ccgtcggatc gaaccgcgat gagttcatca 
-070 tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa 
0001 atgaaaatcc gttcacaagt cggcatggtg cttaacctcg acaaatgtat cggctgccat acctgttcgg 
0071 tgacctgtaa aaacgtctgg accgggcgcg aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa 
0141 accgggcatt ggttatccga aaaactggga agatcaggaa gagtggcaag gcggctgggt gcgtgatgtg 
0211 aatggcaaga tacgcccgcg tctgggcaac aagatgggcg taataaccaa aatcttcgcc aacccggtgg 
0281 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg caccggaagg 
0351 caaacatatt cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt gatctggggg 
0421 ccaaactggg aagaactgct gggcggtgag ttcgaaaaac gtgcccgcga ccgcaacttc gaggccatgc 
0491 aaaaggagat gtacgggcag tttgaaaaca ccttcatgat gtacctgccg cgcctgtgcg aacactgcct 
0561 caatcccagc tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg aagaagacgg cattgtgctg 
0631 attgatcagg ataaatgccg tggctggcgt ttgtgcataa gcggttgtcc gtacaaaaaa atctacttca 
0701 actggaaaag cggcaagtca gaaaaatgca tcttctgtta cccacgaatt gagtccggtc aaccgaccgt 
0771 gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga ccgcatcgag 
0841 gaagcggcga gcaccgagcg cgaagttgac ctctatgaac gccagtgcga agtgttcctc gatccacacg 
0911 atccctcagt gatcgaggaa gccctgaaac aaggtattcc acaaaatgtg attgacgctg cccagcgttc 
0981 gccagtctac aaaatggcga tggactggaa actggcacta ccgttgcacc ctgaataccg caccctgccg 
1051 atggtctggt acgttcctcc gctgtcaccg attcagtcct acgcagatgc gggcggtttg ccgaaaagcg 
1121 aaggcgtgct gcccgccatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt tgagtgccgg 
1191 cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc actatatgcg ttcacaaacc 
1261 gtggaaggcg ttactgatac tcgtgccatc gacgaagtag gcctgagcgt cgcccaggtc gaagagatgt 
1331 atcgctatct cgccattgcc aactatgaag atcgttttgt tatcccgacc agccatcggg aaatggcggg 
1401 cgatgccttc gcagaacgca acggctgcgg ttttaccttt ggcgacggtt gccacggttc ggacagtaaa 
1471 ttcaacctgt tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg 
1541 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggacg agctgttgtg ggaatgcaag 
1611 gaggacgcgc tggcgttgat ccgccgcgac gcgcc