MG1655
W3110
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Gene Report : narV
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 20 (LD)  ,  DE(PLK1608)  ,  OCR70,L90-5 (LD 20-1_P/O) (D)  ,  OCR70,L90-6 (LD 20-1_P/O & 20-2TC) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  narV
Related strains
     Strains Search Search strains by gene name:  narV   Search strains by all related name:  narV b1465 chlZ ECK1459 JW1460

General information  (Go to Linear View:)
 Gene Name narV  
 Alternative name b1465,chlZ,ECK1459,JW1460  
 Location, Length 1,533,961 - 1,534,641 (  -  ) ;   33.06 min ; 681 (bp) ,   226 (aa) Go to Linear View
 Product nitrate reductase 2 (NRZ), gamma subunit  
 Operon Name narZYWV  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005506 ; iron ion binding ( narV )
GO:0005886 ; plasma membrane ( narV )
GO:0006118 ; electron transport ( narV )
GO:0006810 ; transport ( narV )
GO:0008940 ; nitrate reductase activity ( narV )
GO:0009325 ; nitrate reductase complex ( narV )
GO:0016020 ; membrane ( narV )
GO:0016021 ; integral to membrane ( narV )
GO:0016491 ; oxidoreductase activity ( narV )
GO:0042128 ; nitrate assimilation ( narV )
GO:0046872 ; metal ion binding ( narV )
 PID 1787738  
 EC number
  (KEGG Pathway)   		1.7.99.4  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) NARV_ECOLI ( P19316 )
    -  Protein name Respiratory nitrate reductase 2 gamma chain  
    -  Synonyms EC 1.7.99.4  
    -  Gene name Name=narV; OrderedLocusNames=b1465, z2247, ECs2068;  
    -  Comments
  • FUNCTION:This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.
  • CATALYTIC ACTIVITY:Nitrite + acceptor = nitrate + reduced acceptor.
  • COFACTOR:Binds 2 hemes per subunit (By similarity).
  • SUBUNIT:Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.
  • SUBCELLULAR LOCATION:Integral membrane protein.
  • SIMILARITY:86% identity to narI (the gamma chain of the first E.coli nitrate reductase enzyme).  
    •   botton Entry name(Acc.no) Q8FHI4 ( Q8FHI4 )
        -  Protein name Respiratory nitrate reductase 2 gamma chain  
        -  Synonyms EC 1.7.99.4  
        -  Gene name Name=narV; OrderedLocusNames=c1897;  
        -  Comments
  • COFACTOR:Zinc (By similarity).
  • SIMILARITY:Belongs to the zinc-containing alcohol dehydrogenase family.  

    •  Linear View (Whole Mode)
    View Location
       1525.0  –  1550.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP narV (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain C, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Respiratory nitrate reductase 2 gamma chain  
             PSI-BLAST Respiratory nitrate reductase 2 gamma chain
        nr     (database updated : 2007.02.20 )
             BLAST cryptic nitrate reductase 2, gamma subunit [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        COG COG2181C 
        EcoCyc narV 

    MMBR References
    Mol Gen Genet. 1990;222(1):104-11.
    Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.
    Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M. ( 2233673 )
    Mol Microbiol. 1992;6(2):221-30.
    Involvement of the narJ or narW gene product in the formation of active nitrate reductase in Escherichia coli.
    Blasco F, Pommier J, Augier V, Chippaux M, Giordano G. ( 1545706 )

    Sequences
    Amino acid
    FASTA format 
    0001 MIQYLNVFFY DIYPYICATV FFLGSWLRYD YGQYTWRASS SQMLDKRGMV IWSNLFHIGI LGIFFGHLFG 
0071 MLTPHWMYAW FLPVAAKQLM AMVLGGICGV LTLIGGAGLL WRRLTNQRVR ATSTTPDIII MSILLIQCLL 
0141 GLSTIPFSAQ YPDGSEMMKL VGWAQSIVTF RGGSSEMLNG VAFVFRLHLV LGMTIFLLFP FTRLVHVWSA 
0211 PFEYFTRRYQ IVRSRR
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             acgccacggc gtgtgacagc tcgccgctta 
-070 atcaatatca gcgacgcttt agccaggatg tcgcgccgca atatgtcgac atcagtgcgg gaggtgggaa 
0001 atgattcagt atctgaacgt ctttttttac gatatctacc cgtacatttg tgcgacggtg tttttcctcg 
0071 gcagctggct gcgctacgac tacgggcagt acacctggcg cgcctcctca agtcaaatgc tcgataaacg 
0141 cgggatggtg atatggtcga atctgttcca tatcggcatt ttggggattt tcttcgggca cttgttcggc 
0211 atgttaacgc cgcactggat gtacgcgtgg tttttgccag tggcagcgaa acaactcatg gcgatggtgc 
0281 tcggcggtat ttgcggtgtg ttgacgttga ttggcggcgc aggactgctg tggcgcaggc tgacaaatca 
0351 gcgcgtgcgt gccacttcca ctacgccgga tatcatcatc atgagcattt tgctgatcca gtgtctgttg 
0421 gggctaagta ccataccgtt ttcagcacag tatcctgatg gtagcgaaat gatgaagctg gtgggttggg 
0491 cgcaaagcat tgtcactttc cgtggtggat cgtcagagat gctcaacggc gtagcgttcg tcttccgcct 
0561 gcatctggtg ttgggaatga ccatcttcct gctcttcccg ttcacccgac tggtgcatgt gtggagcgcg 
0631 ccgtttgagt actttactcg tcgatatcaa attgtgcgtt cgcggcgata atttcaccct ggccggatag 
0701 cccttcgctc tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa tgaaaccgca 
0771 ggtgtaccac g