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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
|
Biosci Biotechnol Biochem. 2003;67(4):919-22.
Indispensability of the Escherichia coli carbonic anhydrases YadF and CynT in cell proliferation at a low CO2 partial pressure.
Hashimoto M, Kato J.
(
12784642
)
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|
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Deletion
|
OCR32
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
can
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Related strains
|
|
Strains Search
|
Search strains by gene name:
can
Search strains by all related name:
can b0126 ECK0125 f220 JW0122 yadF
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General information (Go to Linear View: )
|
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Gene Name
|
can
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Alternative name
|
b0126,ECK0125,f220,JW0122,yadF
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|
Location, Length
|
142,008 - 142,670
(
-
)
;
3.06
min
;
663
(bp) ,
220
(aa)
|
|
Product
|
carbonic anhydrase
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Operon Name
|
can
|
|
Note
|
putative carbonic anhdrase
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Function
|
enzyme; Central intermediary metabolism: Pool, multipurpose conversions
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Gene Ontology
|
GO:0004089
;
carbonate dehydratase activity ( can )
GO:0005515
;
protein binding ( can )
GO:0008270
;
zinc ion binding ( can )
GO:0015976
;
carbon utilization ( can )
GO:0016829
;
lyase activity ( can )
GO:0046872
;
metal ion binding ( can )
|
|
PID
|
1786318
|
EC number
(KEGG Pathway)
|
4.2.1.1
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|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
CAN_ECOLI
(
P61517
;
P36857; P75656; Q8KJQ4
)
|
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- Protein name
|
Carbonic anhydrase 2
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- Synonyms
|
EC 4.2.1.1
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- Gene name
|
Name=can; Synonyms=cynT2; OrderedLocusNames=b0126;
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- Comments
|
CATALYTIC ACTIVITY:H(2)CO(3) = CO(2) + H(2)O.
COFACTOR:Binds 1 zinc ion per molecule.
SUBUNIT:Homodimer.
SIMILARITY:Belongs to the plant and prokaryotic carbonic anhydrase family.
CAUTION:Ref.1 sequence differs from that shown due to a frameshift in position 181.
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|
Entry name(Acc.no)
|
Q8FL37
(
Q8FL37
)
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- Protein name
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Protein yadF.
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- Synonyms
|
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- Gene name
|
Name=yadF; OrderedLocusNames=c0155;
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- Comments
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SIMILARITY:Belongs to the ABC transporter family.
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Entry name(Acc.no)
|
Q8X938
(
Q8X938
)
|
|
- Protein name
|
Putative carbonic anhdrase
|
|
- Synonyms
|
EC 4.2.1.1
|
|
- Gene name
|
Name=yadF; OrderedLocusNames=z0137;
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- Comments
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SIMILARITY:Contains 1 HTH lysR-type DNA-binding domain.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
can
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of E. Coli Beta Carbonic Anhydrase (Ecca)
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Carbonic anhydrase 2
|
|
PSI-BLAST
|
Carbonic anhydrase 2
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
carbonic anhydrase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
PROK_CO2_ANHYDRASE_1
PROK_CO2_ANHYDRASE_2
|
| Sequences |
Amino acid
FASTA format
|
0001 MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV
0071 IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE
0141 RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS
0211 NLKLKHANHK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 acttacctta cgttcttaca ctttatctgt
-070 ggtttaaatc gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc gttaacctcc
0001 atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat gctggtggaa gaggatcccg
0071 ggttttttga gaaactggca caagcgcaaa aaccgcgctt tctatggatt ggatgttccg acagtcgcgt
0141 tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc gtaatgttgc taacctggtc
0211 attcacactg acctgaactg cctttccgtg gttcagtatg cagtggatgt actcgaagtt gaacacatta
0281 ttatctgtgg ccactacggt tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa
0351 caactggctg ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcggcgaaat gccgcaagag
0421 cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa cctgggccac tccaccatta
0491 tgcaatcagc gtggaaacgc gggcagaaag ttaccattca cggctgggcc tacggcattc acgacggctt
0561 gctgcgtgat ctggatgtta ccgccaccaa ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc
0631 aacctcaagc tgaaacacgc caaccacaaa taaaaatgcc atgccggatg caacacatcc ggcaacttca
0701 cacttactcg tccagcagaa tcactttgcc gatatacggc agatgacggt aacgctgtgc gta
|
