MG1655
W3110
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Gene Report : narI
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V. ( 8736541 )
     Deletion OCR67 (D)  ,  OCR67-3 (D)  ,  OCR67-5 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  narI
Related strains
     Strains Search Search strains by gene name:  narI   Search strains by all related name:  narI b1227 chlI ECK1221 JW1218

General information  (Go to Linear View:)
 Gene Name narI  
 Alternative name b1227,chlI,ECK1221,JW1218  
 Location, Length 1,285,072 - 1,285,749 (  +  ) ;   27.70 min ; 678 (bp) ,   225 (aa) Go to Linear View
 Product nitrate reductase 1, gamma (cytochrome b(NR)) subunit  
 Operon Name narGHJI  
 Note nitrate reductase 1, cytochrome b(NR), gamma subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0017004 - cytochrome complex assembly  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005506; iron ion binding ( narI )
GO:0005886; plasma membrane ( narI )
GO:0006118; electron transport ( narI )
GO:0006810; transport ( narI )
GO:0008940; nitrate reductase activity ( narI )
GO:0009325; nitrate reductase complex ( narI )
GO:0016020; membrane ( narI )
GO:0016021; integral to membrane ( narI )
GO:0016491; oxidoreductase activity ( narI )
GO:0042128; nitrate assimilation ( narI )
GO:0046872; metal ion binding ( narI )
 PID 1787480  
 EC number
  (KEGG Pathway)
 
1.7.99.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene narI ( EG10640 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) NARI_ECOLI ( P11350 )
    -  Protein name Respiratory nitrate reductase 1 gamma chain  
    -  Synonyms EC 1.7.99.4, Nitrate reductase A gamma subunit, Quinol-nitrate oxidoreductase gamma subunit, Cytochrome B-NR  
    -  Gene name Name=narI; Synonyms=chlI; OrderedLocusNames=b1227;  
    -  Comments
  • FUNCTION:The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.
  • CATALYTIC ACTIVITY:Nitrite + acceptor = nitrate + reduced acceptor.
  • COFACTOR:Binds 2 hemes per subunit.
  • SUBUNIT:Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.
  • SUBCELLULAR LOCATION:Integral membrane protein.
  • INDUCTION:By nitrate.
  • DATABASE:NAME=Worthington enzyme manual; WWW="http://www.worthington-biochem.com/NAR/".  
  •   botton Entry name(Acc.no) Q8FHY6 ( Q8FHY6 )
        -  Protein name Respiratory nitrate reductase 1 gamma chain  
        -  Synonyms EC 1.7.99.4  
        -  Gene name Name=narI; OrderedLocusNames=c1688;  
        -  Comments
  • CATALYTIC ACTIVITY:ATP + thymidine = ADP + thymidine 5'-phosphate.
  • SIMILARITY:Belongs to the thymidine kinase family.  
  •   botton Entry name(Acc.no) Q8XDC5 ( Q8XDC5 )
        -  Protein name Nitrate reductase 1, cytochrome b(NR), gamma subunit.  
        -  Synonyms  
        -  Gene name Name=narI; OrderedLocusNames=z2004;  
        -  Comments
  • SUBCELLULAR LOCATION:Periplasmic (By similarity).
  • SIMILARITY:Belongs to the periplasmic pilus chaperone family.  

  •  Linear View (Whole Mode)
    View Location
       1275.0  –  1300.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP narI (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain C, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Respiratory nitrate reductase 1 gamma chain  
             PSI-BLAST Respiratory nitrate reductase 1 gamma chain
        nr     (database updated : 2007.02.20 )
             BLAST nitrate reductase 1, gamma (cytochrome b(NR)) subunit [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

    Other Cross-Reference
        COG COG2181C 
        EcoCyc narI 

    MMBR References
    J Bacteriol. 1983;154(2):719-27.
    Membrane cytochromes of Escherichia coli chl mutants.
    Hackett NR, Bragg PD. ( 6302081 )
    J Bacteriol. 1991;173(5):1574-5.
    Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
    Leonardo MR, Clark DP. ( 1999382 )
    J Gen Microbiol. 1980;117(1):257-62.
    A mutant defective in electron transfer to nitrate in Escherichia coli K12.
    Orth V, Chippaux M, Pascal MC. ( 6993626 )
    J Bacteriol. 1984;159(1):159-66.
    Construction in vitro of a cloned nar operon from Escherichia coli.
    Rondeau SS, Hsu PY, DeMoss JA. ( 6330027 )
    J Bacteriol. 1988;170(4):1721-9.
    narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
    Sodergren EJ, DeMoss JA. ( 2832376 )
    J Biol Chem. 1988;263:16156-16162
    Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.
    Sodergren, E. J., P. Y. Hsu, and J. A. DeMoss.
    J Bacteriol. 1982;151(2):788-99.
    Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.
    Stewart V, MacGregor CH. ( 7047497 )
    Mol Gen Genet. 1981;181(4):535-40.
    Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon.
    Bonnefoy-Orth V, Lepelletier M, Pascal MC, Chippaux M. ( 7022129 )
    Mol Gen Genet. 1981;182(3):477-9.
    Operon fusions in the nitrate reductase operon and study of the control gene nir R in Escherichia coli.
    Chippaux M, Bonnefoy-Orth V, Ratouchniak J, Pascal MC. ( 6457962 )

    Sequences
    Amino acid
    FASTA format
    0001 MQFLNMFFFD IYPYIAGAVF LIGSWLRYDY GQYTWRAASS QMLDRKGMNL ASNLFHIGIL GIFVGHFFGM 
    0071 LTPHWMYEAW LPIEVKQKMA MFAGGASGVL CLIGGVLLLK RRLFSPRVRA TTTGADILIL SLLVIQCALG 
    0141 LLTIPFSAQH MDGSEMMKLV GWAQSVVTFH GGASQHLDGV AFIFRLHLVL GMTLFLLFPF SRLIHIWSVP 
    0211 VEYLTRKYQL VRARH
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             ACAAAGGCTG CGGTGATTCA GCAATCACTG 
    -070 CGCATCAGCG TCGCTTTGCC GGTGCCGTCG CGCCGCAATA TCTGAATATC ACCACCGGAG GACAGCACTA 
    0001 atgcaattcc tgaatatgtt cttctttgat atctacccgt acatagccgg ggcggtcttc ctgattggta 
    0071 gctggctgcg ttatgactac gggcagtaca cctggcgcgc ggcgtccagc cagatgctgg atcgcaaagg 
    0141 gatgaacctg gcgtcgaacc tgttccatat cgggattctg gggatttttg tcggtcactt cttcggtatg 
    0211 ctgacgccgc actggatgta tgaagcctgg ctgccgattg aagtgaaaca gaaaatggca atgtttgctg 
    0281 gtggtgccag cggcgtgctg tgtctgattg gcggcgtgct gttgctgaaa cgtcgtctgt tcagcccacg 
    0351 cgtgcgtgca accactaccg gagcggatat cctgatcctg tcgctgctcg ttatccagtg cgcgctgggc 
    0421 ctgttgacca ttccgttctc cgctcagcat atggacggta gcgagatgat gaaactggtt ggctgggcgc 
    0491 agtcggtggt gaccttccac ggtggcgctt ctcaacacct cgatggtgtg gcgtttatct tccgtctgca 
    0561 cctggtgctg gggatgacgt tattcctgct gttcccgttc tcgcgtctga tacacatctg gagcgtaccg 
    0631 gtggagtatc tgacacgtaa gtaccagctg gtgcgcgctc gtcactaaGC GAATTTTAGT TCACATAGAC 
    0701 CCTGCTTCGG CGGGGTTTTT TTATGGGCAC GGTGCGGGGT GAGTTGTCGG ATGCGCTTCG CTTATCCGAC 
    0771 CTACAGGG