MG1655
W3110
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Gene Report : narH
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V. ( 8736541 )
     Deletion OCR67 (D)  ,  OCR67-3 (D)  ,  OCR67-5 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  narH
Related strains
     Strains Search Search strains by gene name:  narH   Search strains by all related name:  narH b1225 chlC ECK1219 JW1216

General information  (Go to Linear View:)
 Gene Name narH  
 Alternative name b1225,chlC,ECK1219,JW1216  
 Location, Length 1,282,827 - 1,284,365 (  +  ) ;   27.65 min ; 1539 (bp) ,   512 (aa) Go to Linear View
 Product nitrate reductase 1, beta (Fe-S) subunit  
 Operon Name narGHJI  
 Note nitrate reductase 1, beta subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005506 ; iron ion binding ( narH )
GO:0005515 ; protein binding ( narH )
GO:0005886 ; plasma membrane ( narH )
GO:0006118 ; electron transport ( narH )
GO:0006810 ; transport ( narH )
GO:0008940 ; nitrate reductase activity ( narH )
GO:0009055 ; electron carrier activity ( narH )
GO:0009325 ; nitrate reductase complex ( narH )
GO:0016020 ; membrane ( narH )
GO:0016491 ; oxidoreductase activity ( narH )
GO:0042126 ; nitrate metabolic process ( narH )
GO:0042128 ; nitrate assimilation ( narH )
GO:0046872 ; metal ion binding ( narH )
GO:0051536 ; iron-sulfur cluster binding ( narH )
GO:0051538 ; 3 iron, 4 sulfur cluster binding ( narH )
GO:0051539 ; 4 iron, 4 sulfur cluster binding ( narH )
 PID 1787478  
 EC number
  (KEGG Pathway)   		1.7.99.4  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene narH ( EG10639 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) NARH_ECOLI ( P11349 )
    -  Protein name Respiratory nitrate reductase 1 beta chain  
    -  Synonyms EC 1.7.99.4, Nitrate reductase A beta subunit, Quinol-nitrate oxidoreductase beta subunit  
    -  Gene name Name=narH; OrderedLocusNames=b1225;  
    -  Comments
  • FUNCTION:The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.
  • CATALYTIC ACTIVITY:Nitrite + acceptor = nitrate + reduced acceptor.
  • COFACTOR:Binds 3 4Fe-4S clusters and 1 3Fe-4S cluster per subunit.
  • SUBUNIT:Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.
  • SUBCELLULAR LOCATION:Membrane-associated.
  • INDUCTION:By nitrate.
  • DATABASE:NAME=Worthington enzyme manual; WWW="http://www.worthington-biochem.com/NAR/".  
    •   botton Entry name(Acc.no) Q8FHY8 ( Q8FHY8 )
        -  Protein name Respiratory nitrate reductase 1 beta chain  
        -  Synonyms EC 1.7.99.4  
        -  Gene name Name=narH; OrderedLocusNames=c1686;  
        -  Comments
  • CATALYTIC ACTIVITY:ATP + thymidine = ADP + thymidine 5'-phosphate.
  • SIMILARITY:Belongs to the thymidine kinase family.  
    •   botton Entry name(Acc.no) Q8XDC6 ( Q8XDC6 )
        -  Protein name Nitrate reductase 1, beta subunit.  
        -  Synonyms  
        -  Gene name Name=narH; OrderedLocusNames=z2002;  
        -  Comments
  • SUBCELLULAR LOCATION:Periplasmic (By similarity).
  • SIMILARITY:Belongs to the periplasmic pilus chaperone family.  

    •  Linear View (Whole Mode)
    View Location
       1270.0  –  1295.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP narH (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain B, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Respiratory nitrate reductase 1 beta chain  
             PSI-BLAST Respiratory nitrate reductase 1 beta chain
        nr     (database updated : 2007.02.20 )
             BLAST nitrate reductase 1, beta (Fe-S) subunit [Escherichia coli K12]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    CYTOCHROME_C     

    Other Cross-Reference
        COG COG1140C 
        EcoCyc narH 

    MMBR References
    J Bacteriol. 1983;153(3):1513-20.
    chlC (nar) operon of Escherichia coli includes structural genes for alpha and beta subunits of nitrate reductase.
    Edwards ES, Rondeau SS, DeMoss JA. ( 6298188 )
    FEMS Microbiol Lett. 1981;12:125-129.
    Characterization of the Escherichia coli chlC regulatory region in a cloned chlC-lac gene fusion.
    Fimmel, A. L., and B. A. Haddock.
    Eur J Biochem. 1997;250:567-577
    Kinetics of membrane-bound nitrate reductase A from Escherichia coli with analogues of physiological electron donorsdifferent reaction sites for menadiol and duroquinol.
    Giordani, R., J. Buc, A. Cornish-Bowden, and M. L. Cardenas.
    FEMS Microbiol Lett. 1981;11:213-216.
    Cloning the chlC gene for nitrate reductase of Escherichia coli.
    Barr, G. C., and S. E. Palm-Nicholls.
    J Bacteriol. 1991;173(5):1574-5.
    Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
    Leonardo MR, Clark DP. ( 1999382 )
    Mol Gen Genet. 1989;218(2):249-56.
    Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.
    Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V. ( 2674654 )
    J Bacteriol. 1988;170(4):1721-9.
    narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
    Sodergren EJ, DeMoss JA. ( 2832376 )

    Sequences
    Amino acid
    FASTA format 
    0001 MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TSREGVEYAW FNNVETKPGQ GFPTDWENQE KYKGGWIRKI 
0071 NGKLQPRMGN RAMLLGKIFA NPHLPGIDDY YEPFDFDYQN LHTAPEGSKS QPIARPRSLI TGERMAKIEK 
0141 GPNWEDDLGG EFDKLAKDKN FDNIQKAMYS QFENTFMMYL PRLCEHCLNP ACVATCPSGA IYKREEDGIV 
0211 LIDQDKCRGW RMCITGCPYK KIYFNWKSGK SEKCIFCYPR IEAGQPTVCS ETCVGRIRYL GVLLYDADAI 
0281 ERAASTENEK DLYQRQLDVF LDPNDPKVIE QAIKDGIPLS VIEAAQQSPV YKMAMEWKLA LPLHPEYRTL 
0351 PMVWYVPPLS PIQSAADAGE LGSNGILPDV ESLRIPVQYL ANLLTAGDTK PVLRALKRML AMRHYKRAET 
0421 VDGKVDTRAL EEVGLTEAQA QEMYRYLAIA NYEDRFVVPS SHRELAREAF PEKNGCGFTF GDGCHGSDTK 
0491 FNLFNSRRID AIDVTSKTEP HP
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             CCGTAGGTTC TAACCGCGAT GAGTTTGTTG 
-070 TAGTGCGTAA GATGAAGAAC ATTGACTGGT TAGATGGCGA AGGCAATGAC CAGGTACAGG AGAGCGTAAA 
0001 atgaaaattc gttcacaagt cggcatggtg ctgaatctcg ataagtgcat cggctgccac acctgttcag 
0071 ttacctgtaa aaacgtctgg accagccgtg aaggcgtgga atatgcgtgg ttcaacaacg tggaaaccaa 
0141 gccgggccag ggcttcccga ctgactggga aaaccaggaa aaatacaaag gcggctggat ccgtaaaatc 
0211 aacggcaaac tgcagccgcg catgggtaac cgtgccatgc tgctgggtaa aatcttcgct aacccgcatc 
0281 tgccggggat cgacgattat tacgagccgt tcgattttga ctatcagaac ctgcataccg cgccggaagg 
0351 cagcaaatcg cagccgattg cccgtccgcg ttcgctgatt accggggaac ggatggcgaa aatcgaaaaa 
0421 gggccgaact gggaagatga cctgggcggt gagtttgaca aactggcgaa agacaagaac ttcgacaaca 
0491 tccagaaggc gatgtatagc cagttcgaaa acaccttcat gatgtatttg ccgcgcctgt gcgaacactg 
0561 cctgaacccg gcatgtgtgg cgacctgccc gagcggtgcg atttacaagc gtgaagaaga tggcatcgtc 
0631 ctgatcgacc aggataaatg ccgtggctgg cgtatgtgca tcactggatg cccgtacaaa aaaatctact 
0701 tcaactggaa gagcggtaag tctgagaagt gcatcttctg ctatccgcgt attgaagcgg gtcagccgac 
0771 cgtgtgctca gaaacctgtg tcggtcgtat ccgttatctt ggcgtgctgt tgtacgatgc cgacgctatt 
0841 gaacgtgcag ccagcaccga gaacgagaaa gatctttacc agcgtcagct ggacgtgttc ctcgatccga 
0911 acgatccgaa agtcatcgag caggcgatta aagacggtat tccgctgagc gttattgaag ccgcacagca 
0981 gtcgccggtt tataaaatgg caatggaatg gaaactggcg ctgccgctgc atccggaata tcgcacactg 
1051 ccgatggtct ggtacgtgcc gcctctgtct ccgattcagt ctgcagcaga cgcgggtgag ctgggtagca 
1121 acggcattct gccagacgtc gaaagcctgc gtattccggt acagtatctg gcgaatctgc tgaccgccgg 
1191 tgataccaaa ccggtactgc gcgcactgaa acgtatgctg gcgatgcgtc attacaaacg tgctgaaacc 
1261 gttgacggta aagttgatac ccgtgcgctg gaagaggtcg gtctgaccga agcccaggca caggagatgt 
1331 accgttatct ggcgattgct aactacgaag atcgctttgt ggtgccgagt agtcatcgtg aactggcacg 
1401 ggaagccttc ccggagaaaa atggctgcgg ctttaccttt ggtgatggct gccacggttc agataccaaa 
1471 ttcaatctgt tcaacagccg tcgtatcgat gccatcgatg tgaccagcaa aacggagccg catccatgaT 
1541 CGAACTCGTG ATTGTATCGC GTCTCCTTGA ATATCCGGAT GCTGCCTTAT GGCAGCATCA ACAAGAGATG 
1611 TTTGAGGCGA TTGCCGCGTC GAAAAATCT