|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Appl Environ Microbiol. 1995;61(7):2487-92.
Role of fadR and atoC(Con) mutations in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha+ Escherichia coli.
Rhie HG, Dennis D.
(
7618860
)
|
|
|
Deletion
|
OCR65
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
fadR
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
fadR
Search strains by all related name:
fadR b1187 dec ECK1175 JW1176 ole oleR thdB
|
General information (Go to Linear View: )
|
|
Gene Name
|
fadR
|
|
Alternative name
|
b1187,dec,ECK1175,JW1176,ole,oleR,thdB
|
|
Location, Length
|
1,234,161 - 1,234,880
(
+
)
;
26.60
min
;
720
(bp) ,
239
(aa)
|
|
Product
|
DNA-binding transcriptional dual regulator of fatty acid metabolism
|
|
Operon Name
|
fadR
|
|
Note
|
negative regulator for fad regulon, and positive activator of fabA; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0019395 - fatty acid oxidation; GO_process: GO:0006350 - transcription
|
|
Function
|
regulator; Degradation of small molecules: Fatty acids
|
|
Gene Ontology
|
GO:0000062
;
acyl-CoA binding ( fadR )
GO:0003677
;
DNA binding ( fadR )
GO:0003700
;
transcription factor activity ( fadR )
GO:0005622
;
intracellular ( fadR )
GO:0005737
;
cytoplasm ( fadR )
GO:0006350
;
transcription ( fadR )
GO:0006355
;
regulation of transcription, DNA-dependent ( fadR )
GO:0006629
;
lipid metabolic process ( fadR )
GO:0006631
;
fatty acid metabolic process ( fadR )
GO:0019217
;
regulation of fatty acid metabolic process ( fadR )
GO:0045449
;
regulation of transcription ( fadR )
|
|
PID
|
1787436
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
fadR
(
EG10281
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
FADR_ECOLI
(
P09371
;
P76827
)
|
|
- Protein name
|
Fatty acid metabolism regulator protein.
|
|
- Synonyms
|
|
|
- Gene name
|
OrderedLocusNames=b1187, c1635, z1950, ECs1682, SF1176, S1264;
|
|
- Comments
|
FUNCTION:Multifunctional regulator of fatty acid metabolism. Represses transcription of at least eight genes required for fatty acid transport and beta-oxidation among which fadA, fadB, fadD, fadL and fadE. Activates transcription of at least three genes required for unsaturated fatty acid biosynthesis:fabA, fabB and iclR, the gene encoding the transcriptional regulator of the aceBAK operon encoding the glyoxylate shunt enzymes. Binding of fadR is specifically inhibited by long chain fatty acyl-CoA compounds.
SUBUNIT:Homodimer.
SUBCELLULAR LOCATION:Cytoplasmic (Potential).
DOMAIN:The C-terminal domain bind acyl-CoA.
SIMILARITY:Contains 1 HTH gntR-type DNA-binding domain.
|
|
MMBR References
|
Nucleic Acids Res. 1988;16:7995-8009
Nucleotide sequence of the fadR gene, a multifunctional regulator of fatty acid metabolism in Escherichia coli.
DiRusso, C. C.
|
J Bacteriol. 1985;161(2):583-8.
Cloning and characterization of a gene (fadR) involved in regulation of fatty acid metabolism in Escherichia coli.
DiRusso CC, Nunn WD.
(
3881402
)
|
J Bacteriol. 1996;178:6443-6450
Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes.
Farewell, A., A. A. Diez, C. C. DiRusso, and T. Nystrom.
|
J Bacteriol. 1990;172(12):6618
Location of icdA and fadR on the physical map of Escherichia coli.
Herold C, Birge EA.
(
2254241
)
|
J Bacteriol. 1982;149(1):173-80.
Genetic regulation of the glyoxylate shunt in Escherichia coli K-12.
Maloy SR, Nunn WD.
(
7033207
)
|
J Bacteriol. 1987;169(3):1267-71.
Isolation and genetic analysis of mutations allowing the degradation of furans and thiophenes by Escherichia coli.
Abdulrashid N, Clark DP.
(
3546268
)
|
J Bacteriol. 1980;143(2):726-30.
Regulation of fatty acid degradation in Escherichia coli: dominance studies with strains merodiploid in gene fadR.
Simons RW, Hughes KT, Nunn WD.
(
7009562
)
|
J Bacteriol. 1980;142(2):621-32.
Regulation of fatty acid degradation in Escherichia coli: isolation and characterization of strains bearing insertion and temperature-sensitive mutations in gene fadR.
Simons RW, Egan PA, Chute HT, Nunn WD.
(
6247326
)
|
Mol Gen Genet. 1985;198(2):315-22.
Identification of the dadX gene coding for the predominant isozyme of alanine racemase in Escherichia coli K12.
Wild J, Hennig J, Lobocka M, Walczak W, Klopotowski T.
(
3920477
)
|
J Bacteriol. 1981;148(2):521-6.
Regulation of fatty acid degradation in Escherichia coli: analysis by operon fusion.
Clark D.
(
6271734
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MVIKAQSPAG FAEEYIIESI WNNRFPPGTI LPAERELSEL IGVTRTTLRE VLQRLARDGW LTIQHGKPTK
0071 VNNFWETSGL NILETLARLD HESVPQLIDN LLSVRTNIST IFIRTAFRQH PDKAQEVLAT ANEVADHADA
0141 FAELDYNIFR GLAFASGNPI YGLILNGMKG LYTRIGRHYF ANPEARSLAL GFYHKLSALC SEGAHDQVYE
0211 TVRRYGHESG EIWHRMQKNL PGDLAIQGR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AGCCTTGATC CCTTTTTCTT CTTTTTGTCT
-070 GCTATCAGCG TAGTTAGCCC TCTGGTATGA TGAGTCCAAC TTTGTTTTGC TGTGTTATGG AAATCTCACT
0001 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc tggaataacc
0071 gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta attggcgtaa cgcgtactac
0141 gttacgtgaa gtgttacagc gtctggcacg agatggctgg ttgaccattc aacatggcaa gccgacgaag
0211 gtgaataatt tctgggaaac ttccggttta aatatccttg aaacactggc gcgactggat cacgaaagtg
0281 tgccgcagct tattgataat ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt
0351 tcgtcagcat cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
0421 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt tacggtctga
0491 ttcttaacgg gatgaaaggg ctgtatacgc gtattggtcg tcactatttc gccaatccgg aagcgcgcag
0561 tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg cgcacgatca ggtgtacgaa
0631 acagtgcgtc gctatgggca tgagagtggc gagatttggc accggatgca gaaaaatctg ccgggtgatt
0701 tagccattca ggggcgataa TCCCTTCCGT TTAAAGAGCA AACCCCTCAA ACGAGGGGTT TTTTGTTGTT
0771 TTTACAGATT TCCCATTCTT GGCGGGCAAC GTTCCAGCAA CTCGATGCTG
|
