|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Microbiology. 1996;142 (Pt 11):3219-30.
The purB gene of Escherichia coli K-12 is located in an operon.
Green SM, Malik T, Giles IG, Drabble WT.
(
8969519
)
|
|
|
Deletion
|
OCR63
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
hflD
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
hflD
Search strains by all related name:
hflD asuE b1132 ECK1118 hslZ JW5165 trmU ycfC
|
General information (Go to Linear View: )
|
|
Gene Name
|
hflD
|
|
Alternative name
|
asuE,b1132,ECK1118,hslZ,JW5165,trmU,ycfC
|
|
Location, Length
|
1,191,213 - 1,191,854
(
-
)
;
25.67
min
;
642
(bp) ,
213
(aa)
|
|
Product
|
putative lysogenization regulator
|
|
Operon Name
|
hflD-purB
|
|
Note
|
GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane
|
|
Function
|
|
|
Gene Ontology
|
GO:0004808
;
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity ( trmU )
GO:0005737
;
cytoplasm ( trmU )
GO:0006400
;
tRNA modification ( trmU )
GO:0008033
;
tRNA processing ( trmU )
GO:0008168
;
methyltransferase activity ( trmU )
GO:0016740
;
transferase activity ( trmU )
|
|
PID
|
1787377
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
TRMU_ECOLI
(
P25745
;
P75964
)
|
|
- Protein name
|
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
|
|
- Synonyms
|
EC 2.1.1.61
|
|
- Gene name
|
Name=trmU; Synonyms=asuE, mnmA; OrderedLocusNames=b1133;
|
|
- Comments
|
CATALYTIC ACTIVITY:S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.
SUBCELLULAR LOCATION:Cytoplasmic.
SIMILARITY:Belongs to the trmU family.
CAUTION:Ref.3 sequence differs from that shown due to frameshifts.
|
|
Entry name(Acc.no)
|
YCFC_ECOLI
(
P25746
)
|
|
- Protein name
|
Hypothetical UPF0274 protein ycfC
|
|
- Synonyms
|
ORF-23
|
|
- Gene name
|
Name=ycfC; OrderedLocusNames=b1132;
|
|
- Comments
|
SIMILARITY:Belongs to the UPF0274 family.
CAUTION:Ref.2 sequence differs from that shown due to frameshifts.
|
|
Entry name(Acc.no)
|
TRMU_ECOL6
(
Q8CXZ8
)
|
|
- Protein name
|
Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
|
|
- Synonyms
|
EC 2.1.1.61
|
|
- Gene name
|
Name=trmU; OrderedLocusNames=c1512;
|
|
- Comments
|
CATALYTIC ACTIVITY:S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the trmU family.
|
|
Entry name(Acc.no)
|
YCFC_ECOL6
(
Q8FIB7
)
|
|
- Protein name
|
Hypothetical UPF0274 protein ycfC.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=ycfC; OrderedLocusNames=c1511;
|
|
- Comments
|
SIMILARITY:Belongs to the UPF0274 family.
|
|
Entry name(Acc.no)
|
TRMU_ECO57
(
Q8X735
)
|
|
- Protein name
|
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
|
|
- Synonyms
|
EC 2.1.1.61
|
|
- Gene name
|
Name=trmU; OrderedLocusNames=z1862, ECs1605;
|
|
- Comments
|
CATALYTIC ACTIVITY:S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.
SUBCELLULAR LOCATION:Cytoplasmic (By similarity).
SIMILARITY:Belongs to the trmU family.
|
|
Entry name(Acc.no)
|
YCFC_ECO57
(
Q8X736
)
|
|
- Protein name
|
Hypothetical UPF0274 protein ycfC.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=ycfC; OrderedLocusNames=z1861, ECs1604;
|
|
- Comments
|
SIMILARITY:Belongs to the UPF0274 family.
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
hflD
(
homologous genes of other bacterias
)
trmU
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Structure Of Hypothetical Escherichia Coli Protein Ycfc
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
UPF0274 protein ycfC
|
|
PSI-BLAST
|
UPF0274 protein ycfC
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
hypothetical protein b1132 [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
LEUCINE_ZIPPER
|
|
MMBR References
|
Microbiology 1996;142:3219-3230
The purB gene of Escherichia coli K-12 is located in an operon.
Green, S. M., T. Malik, I. G. Giles, and W. T. Drabble.
|
Biochimie. 1995;77(1-2):66-74.
Aminoacylation of transfer RNAs with 2-thiouridine derivatives in the wobble position of the anticodon.
Rogers KC, Crescenzo AT, Soll D.
(
7541255
)
|
J Bacteriol. 1985;161(1):368-76.
Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine.
Sullivan MA, Cannon JF, Webb FH, Bock RM.
(
3881393
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAKNYYDITL ALAGICQSAR LVQQLAHQGH CDADALHVSL NSIIDMNPSS TLAVFGGSEA NLRVGLETLL
0071 GVLNASSRQG LNAELTRYTL SLMVLERKLS SAKGALDTLG NRINGLQRQL EHFDLQSETL MSAMAAIYVD
0141 VISPLGPRIQ VTGSPAVLQS PQVQAKVRAT LLAGIRAAVL WHQVGGGRLQ LMFSRNRLTT QAKQILAHLT
0211 PEL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tctataacgg tgaagtgtgc ctcggtggcg
-070 gtattattga gcagcgtctg ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac
0001 gtggcaaaga attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
0071 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta ttgatatgaa
0141 ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg tcgggctgga aaccctgctc
0211 ggcgtgctca atgccagcag tcgccagggc ttaaacgccg aattaacccg ctacacactc agcttgatgg
0281 tgcttgagcg caaactctcc tcagcgaaag gcgcgctcga cactctgggc aaccggatca acggcctgca
0351 acgccagctc gaacacttcg atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat
0421 gtgattagcc cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
0491 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg tcggcggcgg
0561 acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac aaattcttgc tcatttaacc
0631 ccggagttgt gatctatgga attatcctca ctgaccgccg tttcccctgt cgatggacgc tacggcgata
0701 aagtcagcgc gctgcgcggg attttcagcg aatatggttt gc
|
