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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V.
(
8736541
)
|
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Deletion
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OCR67
(D)
,
OCR67-3
(D)
,
OCR67-5
(D)
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|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
narG
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Related strains
|
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Strains Search
|
Search strains by gene name:
narG
Search strains by all related name:
narG b1224 chlC ECK1218 JW1215 narC
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General information (Go to Linear View: )
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Gene Name
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narG
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Alternative name
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b1224,chlC,ECK1218,JW1215,narC
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Location, Length
|
1,279,087 - 1,282,830
(
+
)
;
27.57
min
;
3744
(bp) ,
1247
(aa)
|
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Product
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nitrate reductase 1, alpha subunit
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Operon Name
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narGHJI
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Note
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GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration
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Function
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enzyme; Energy metabolism, carbon: Anaerobic respiration
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Gene Ontology
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GO:0005488
;
binding ( narG )
GO:0005506
;
iron ion binding ( narG )
GO:0005515
;
protein binding ( narG )
GO:0005886
;
plasma membrane ( narG )
GO:0006118
;
electron transport ( narG )
GO:0006810
;
transport ( narG )
GO:0008940
;
nitrate reductase activity ( narG )
GO:0009325
;
nitrate reductase complex ( narG )
GO:0016020
;
membrane ( narG )
GO:0016491
;
oxidoreductase activity ( narG )
GO:0030151
;
molybdenum ion binding ( narG )
GO:0042126
;
nitrate metabolic process ( narG )
GO:0042128
;
nitrate assimilation ( narG )
GO:0046872
;
metal ion binding ( narG )
GO:0051536
;
iron-sulfur cluster binding ( narG )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( narG )
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PID
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1787477
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EC number
(KEGG Pathway)
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1.7.99.4
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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narG
(
EG10638
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
NARG_ECOLI
(
P09152
;
P78294
)
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|
- Protein name
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Respiratory nitrate reductase 1 alpha chain
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- Synonyms
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EC 1.7.99.4, Nitrate reductase A alpha subunit, Quinol-nitrate oxidoreductase alpha subunit
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- Gene name
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Name=narG; Synonyms=narC, bisD; OrderedLocusNames=b1224;
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MMBR References
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J Bacteriol. 1983;153(3):1513-20.
chlC (nar) operon of Escherichia coli includes structural genes for alpha and beta subunits of nitrate reductase.
Edwards ES, Rondeau SS, DeMoss JA.
(
6298188
)
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J Bacteriol. 1983;154(2):719-27.
Membrane cytochromes of Escherichia coli chl mutants.
Hackett NR, Bragg PD.
(
6302081
)
|
FEMS Microbiol Lett. 1981;11:213-216.
Cloning the chlC gene for nitrate reductase of Escherichia coli.
Barr, G. C., and S. E. Palm-Nicholls.
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J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP.
(
1999382
)
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J Bacteriol. 1987;169(10):4614-20.
Promoter region of the nar operon of Escherichia coli: nucleotide sequence and transcription initiation signals.
Li SF, DeMoss JA.
(
3308846
)
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J Bacteriol. 1985;164(1):25-32.
Delineation of two distinct regulatory domains in the 5' region of the nar operon of Escherichia coli.
Li S, Rabi T, DeMoss JA.
(
2995309
)
|
FEBS Lett. 1984;177(2):260-4.
Respiratory nitrate reductase of Escherichia coli. Sequence identification of the large subunit gene.
McPherson MJ, Baron AJ, Pappin DJ, Wootton JC.
(
6094247
)
|
J Bacteriol. 1984;159(1):159-66.
Construction in vitro of a cloned nar operon from Escherichia coli.
Rondeau SS, Hsu PY, DeMoss JA.
(
6330027
)
|
Mol Gen Genet. 1990;222(1):104-11.
Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.
Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M.
(
2233673
)
|
J Bacteriol. 1988;170(4):1721-9.
narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
Sodergren EJ, DeMoss JA.
(
2832376
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKFLDRFRY FKQKGETFAD GHGQLLNTNR DWEDGYRQRW QHDKIVRSTH GVNCTGSCSW KIYVKNGLVT
0071 WETQQTDYPR TRPDLPNHEP RGCPRGASYS WYLYSANRLK YPMMRKRLMK MWREAKALHS DPVEAWASII
0141 EDADKAKSFK QARGRGGFVR SSWQEVNELI AASNVYTIKN YGPDRVAGFS PIPAMSMVSY ASGARYLSLI
0211 GGTCLSFYDW YCDLPPASPQ TWGEQTDVPE SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTVA
0281 VTPDYAEIAK LCDLWLAPKQ GTDAAMALAM GHVMLREFHL DNPSQYFTDY VRRYTDMPML VMLEERDGYY
0351 AAGRMLRAAD LVDALGQENN PEWKTVAFNT NGEMVAPNGS IGFRWGEKGK WNLEQRDGKT GEETELQLSL
0421 LGSQDEIAEV GFPYFGGDGT EHFNKVELEN VLLHKLPVKR LQLADGSTAL VTTVYDLTLA NYGLERGLND
0491 VNCATSYDDV KAYTPAWAEQ ITGVSRSQII RIAREFADNA DKTHGRSMII VGAGLNHWYH LDMNYRGLIN
0561 MLIFCGCVGQ SGGGWAHYVG QEKLRPQTGW QPLAFALDWQ RPARHMNSTS YFYNHSSQWR YETVTAEELL
0631 SPMADKSRYT GHLIDFNVRA ERMGWLPSAP QLGTNPLTIA GEAEKAGMNP VDYTVKSLKE GSIRFAAEQP
0701 ENGKNHPRNL FIWRSNLLGS SGKGHEFMLK YLLGTEHGIQ GKDLGQQGGV KPEEVDWQDN GLEGKLDLVV
0771 TLDFRLSSTC LYSDIILPTA TWYEKDDMNT SDMHPFIHPL SAAVDPAWEA KSDWEIYKAI AKKFSEVCVG
0841 HLGKETDIVT LPIQHDSAAE LAQPLDVKDW KKGECDLIPG KTAPHIMVVE RDYPATYERF TSIGPLMEKI
0911 GNGGKGIAWN TQSEMDLLRK LNYTKAEGPA KGQPMLNTAI DAAEMILTLA PETNGQVAVK AWAALSEFTG
0981 RDHTHLALNK EDEKIRFRDI QAQPRKIISS PTWSGLEDEH VSYNAGYTNV HELIPWRTLS GRQQLYQDHQ
1051 WMRDFGESLL VYRPPIDTRS VKEVIGQKSN GNQEKALNFL TPHQKWGIHS TYSDNLLMLT LGRGGPVVWL
1121 SEADAKDLGI ADNDWIEVFN SNGALTARAV VSQRVPAGMT MMYHAQERIV NLPGSEITQQ RGGIHNSVTR
1191 ITPKPTHMIG GYAHLAYGFN YYGTVGSNRD EFVVVRKMKN IDWLDGEGND QVQESVK
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Nucleotide
FASTA format
View sequence in neighbor 100bp
|
0001 atgagtaaat tcctggaccg gtttcgctac ttcaaacaga agggtgaaac ctttgccgat gggcatggcc
0071 agcttctcaa taccaaccgt gactgggagg atggatatcg ccagcgttgg cagcatgaca aaatcgtccg
0141 ctctacccac ggggtaaact gcaccggctc ctgcagctgg aaaatctacg tcaaaaacgg tctggtcacc
0211 tgggaaaccc agcagactga ctatccgcgt acccgtccgg atctgccaaa ccatgaacct cgcggctgcc
0281 cgcgcggtgc cagctactcc tggtatcttt acagtgccaa ccgcctgaaa tacccgatga tgcgcaaacg
0351 cctgatgaaa atgtggcgtg aagcgaaggc gctgcatagc gatccggttg aggcatgggc ttctatcatt
0421 gaagacgccg ataaagcgaa aagctttaag caggcgcgtg gacgcggtgg atttgttcgt tcttcctggc
0491 aggaggtgaa cgaactgatc gccgcatcta acgtttacac catcaaaaac tacggcccgg accgtgttgc
0561 tggtttctcg ccaattccgg caatgtcgat ggtttcttac gcatcgggtg cacgctatct ctcgctgatt
0631 ggcggtactt gcttaagctt ctacgactgg tactgcgact tgcctcctgc gtctccgcaa acctggggcg
0701 agcaaactga cgtaccggaa tctgctgact ggtacaactc cagctacatc atcgcctggg ggtcaaacgt
0771 gccgcagacg cgtaccccgg atgctcactt ctttactgaa gtgcgttaca aagggaccaa aactgttgcc
0841 gtcacaccag actacgctga aatcgccaaa ctgtgcgatc tgtggctggc accgaaacag ggcaccgatg
0911 cggcaatggc gctggcgatg ggccacgtaa tgctgcgtga attccacctc gacaacccaa gccagtattt
0981 caccgactat gtgcgtcgct acaccgacat gccgatgctg gtgatgctgg aagaacgcga cggttactac
1051 gctgcaggtc gtatgctgcg cgctgctgat ctggttgatg cgctgggcca ggaaaacaat ccggaatgga
1121 aaactgtcgc ctttaatacc aatggcgaaa tggttgcgcc gaacggttct attggcttcc gctggggcga
1191 gaagggcaaa tggaatcttg agcagcgcga cggcaaaact ggcgaagaaa ccgagctgca actgagcctg
1261 ctgggtagcc aggatgagat cgctgaggta ggcttcccgt actttggtgg cgacggcacg gaacacttca
1331 acaaagtgga actggaaaac gtgctgctgc acaaactgcc ggtgaaacgc ctgcaactgg ctgatggcag
1401 caccgccctg gtgaccaccg tttatgatct gacgctggca aactacggtc tggaacgtgg cctgaacgac
1471 gttaactgtg caaccagcta tgacgatgtg aaagcttata ccccggcctg ggccgagcag attaccggcg
1541 tttctcgcag ccagattatt cgcatcgccc gtgaatttgc cgataacgct gataaaacgc acggtcgttc
1611 gatgattatc gtcggtgcgg ggctgaacca ctggtatcac ctcgatatga actatcgtgg tctgatcaac
1681 atgctgattt tctgcggctg tgtcggtcag agcgggggcg gctgggcgca ctatgtaggt caggaaaaac
1751 tgcgtccgca aaccggctgg cagccgctgg cgtttgccct tgactggcag cgtccggcgc gtcacatgaa
1821 cagcacttct tatttctata accactccag ccagtggcgt tatgaaaccg tcacggcgga agagttgctg
1891 tcaccgatgg cggacaaatc ccgctatacc ggacacttga tcgactttaa cgtccgtgcg gaacgcatgg
1961 gctggctgcc gtctgcaccg cagttaggca ctaacccgct gactatcgct ggcgaagcgg aaaaagccgg
2031 gatgaatccg gtggactata cggtgaaatc cctgaaagag ggttccatcc gttttgcggc agaacaacca
2101 gaaaacggta aaaaccaccc gcgcaacctg ttcatctggc gttctaacct gctcggttct tccggtaaag
2171 gtcatgagtt tatgctcaag tacctgctgg ggacggagca cggtatccag ggtaaagatc tggggcaaca
2241 gggcggcgtg aagccggaag aagtggactg gcaggacaat ggtctggaag gcaagctgga tctggtggtt
2311 acgctggact tccgtctgtc gagcacctgt ctctattccg acatcatttt gccgacggcg acctggtacg
2381 aaaaagacga catgaatact tcggatatgc atccgtttat tcacccgctg tctgcggcgg tcgatccggc
2451 ctgggaagcg aaaagcgact gggaaatcta caaagccatc gcgaagaaat tctccgaagt gtgcgtcggc
2521 catctgggta aagaaaccga catcgtcacg ctgcctatcc agcatgactc tgccgctgaa ctggcgcagc
2591 cgctggatgt gaaagactgg aaaaaaggcg agtgcgacct gatcccaggt aaaaccgcgc cacacattat
2661 ggtcgtagag cgcgattatc cggcgactta cgaacgcttt acctctatcg gcccgctgat ggagaaaatc
2731 ggtaatggcg gtaaagggat tgcctggaac acccagagcg agatggatct gctgcgtaag ctcaactaca
2801 ccaaagcgga aggtccggcg aaaggccagc cgatgctgaa caccgcaatt gatgcggcag agatgatcct
2871 gacactggca ccggaaacca acggtcaggt agccgtgaaa gcctgggctg ccctgagcga atttaccggt
2941 cgtgaccata cgcatctggc gctgaataaa gaagacgaga agatccgctt ccgcgatatt caggcacagc
3011 cgcgcaaaat tatctccagc ccgacctggt ctggtctgga agatgaacac gtttcttaca acgccggtta
3081 caccaacgtt cacgagctga tcccatggcg tacgctctct ggtcgtcagc aactgtatca ggatcaccag
3151 tggatgcgtg atttcggtga aagcctgctg gtttatcgtc cgccgatcga cacccgttcg gtgaaagaag
3221 tgataggcca gaaatccaac ggcaaccagg aaaaagcgct caacttcctg acgccgcacc agaagtgggg
3291 tatccactcc acctacagcg acaacctgct gatgctgact ttaggtcgcg gtggtccggt ggtctggttg
3361 agtgaagccg atgccaaaga tctgggtatc gccgataacg actggattga agtcttcaac agcaacggtg
3431 ctctgactgc ccgtgcggtt gtcagccagc gtgttccggc agggatgacc atgatgtacc acgcgcagga
3501 acgtatcgtt aacctgcctg gttcggaaat tacccaacag cgtggtggta tccataactc ggtcacccgt
3571 atcacgccga aaccgacgca tatgatcggc ggctatgccc atctggcata cggctttaac tactatggca
3641 ccgtaggttc taaccgcgat gagtttgttg tagtgcgtaa gatgaagaac attgactggt tagatggcga
3711 aggcaatgac caggtacagg agagcgtaaa atga
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