MG1655
W3110
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Gene Report : ftsI
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Proc Natl Acad Sci U S A. 1978;75(2):664-8.
On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins.
Suzuki H, Nishimura Y, Hirota Y. ( 345275 )
     Deletion OCR30,31-3 (BK2-1 / AK1-1) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  ftsI
Related strains
     Strains Search Search strains by gene name:  ftsI   Search strains by all related name:  ftsI b0084 ECK0085 JW0082 pbpB sep

General information  (Go to Linear View:)
 Gene Name ftsI  
 Alternative name b0084,ECK0085,JW0082,pbpB,sep  
 Location, Length 91,413 - 93,179 (  +  ) ;   1.97 min ; 1767 (bp) ,   588 (aa) Go to Linear View
 Product transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3)  
 Operon Name mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC  
 Note septum formation; penicillin-binding protein 3; peptidoglycan synthetase; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0042493 - response to drug  
 Function enzyme; Cell division  
 Gene Ontology GO:0003824 ; catalytic activity ( ftsI )
GO:0005886 ; plasma membrane ( ftsI )
GO:0007047 ; cell wall organization and biogenesis ( ftsI )
GO:0007049 ; cell cycle ( ftsI )
GO:0008360 ; regulation of cell shape ( ftsI )
GO:0008658 ; penicillin binding ( ftsI )
GO:0008955 ; peptidoglycan glycosyltransferase activity ( ftsI )
GO:0009252 ; peptidoglycan biosynthetic process ( ftsI )
GO:0009273 ; peptidoglycan-based cell wall biogenesis ( ftsI )
GO:0016020 ; membrane ( ftsI )
GO:0016021 ; integral to membrane ( ftsI )
GO:0016740 ; transferase activity ( ftsI )
GO:0016757 ; transferase activity, transferring glycosyl groups ( ftsI )
GO:0046677 ; response to antibiotic ( ftsI )
GO:0051301 ; cell division ( ftsI )
 PID 1786272  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) FTSI_ECOLI ( P04286 )
    -  Protein name Peptidoglycan synthetase ftsI precursor  
    -  Synonyms EC 2.4.1.129, Peptidoglycan glycosyltransferase 3, Penicillin-binding protein 3, PBP-3  
    -  Gene name Name=ftsI; Synonyms=pbpB; OrderedLocusNames=b0084, z0094, ECs0088;  
    -  Comments
  • FUNCTION:Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates.
  • CATALYTIC ACTIVITY:[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.
  • PATHWAY:Peptidoglycan synthesis; final stages.
  • SUBCELLULAR LOCATION:Inner membrane. The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein.
  • DOMAIN:Has an N-terminal penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a C-terminal penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).
  • MISCELLANEOUS:Mutant pbpBr1, which was obtained after selection for increased resistance to cephalexin, causes a change in the shape of the cell:the polar caps are pointed.
  • SIMILARITY:Belongs to the transpeptidase family.  
    •   botton Entry name(Acc.no) Q8CWE4 ( Q8CWE4 )
        -  Protein name Peptidoglycan synthetase ftsI.  
        -  Synonyms  
        -  Gene name Name=ftsI; OrderedLocusNames=c0102;  
        -  Comments
  • SIMILARITY:Belongs to the N-Me-Phe pili family.  

    •  Linear View (Whole Mode)
    View Location
       80.0  –  105.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP ftsI (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
             PSI-BLAST Chain A, Pbp2x From Streptococcus Pneumoniae Strain 5259 With Reduced Susceptibility To Beta-Lactam Antibiotics  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Peptidoglycan synthetase ftsI precursor (Peptidoglycan glycosyltransferase 3) (Penicillin-binding protein 3) (PBP-3)  
             PSI-BLAST Peptidoglycan synthetase ftsI precursor (Peptidoglycan glycosyltransferase 3) (Penicillin-binding protein 3) (PBP-3)
        nr     (database updated : 2007.02.20 )
             BLAST septum formation; penicillin-binding protein 3; peptidoglycan synthetase [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A     

    Other Cross-Reference
        COG COG0768M 
        EcoCyc ftsI 

    MMBR References
    J Bacteriol. 1996;178:5402-5409
    The non-penicillin-binding module of the tripartite penicillin-binding protein 3 of Escherichia coli is required for folding and/or stability of the penicillin-binding module and the membrane-anchoring module confers cell septation activity on the folded structure.
    Goffin, C., C. Fraipont, J. Ayala, M. Terrak, M. Nguyen-Disteche, and J. M. Ghuysen.
    J Bacteriol. 1997;179:5802-5811
    A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW.
    Hara, H., S. Yasuda, K. Horiuchi, and J. T. Park.
    J Bacteriol. 1989;171(11):6379-82.
    Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
    Jung HK, Ishino F, Matsuhashi M. ( 2553679 )
    J Bacteriol. 1985;163(2):615-22.
    Cell shape and division in Escherichia coli: experiments with shape and division mutants.
    Begg KJ, Donachie WD. ( 3894330 )
    J Bacteriol. 1989;171(11):6126-34.
    Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme.
    Mengin-Lecreulx D, Parquet C, Desviat LR, Pla J, Flouret B, Ayala JA, van Heijenoort J. ( 2681153 )
    Mol Gen Genet. 1983;191(1):1-9.
    On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3.
    Nakamura M, Maruyama IN, Soma M, Kato J, Suzuki H, Horota Y. ( 6350821 )

    Sequences
    Amino acid
    FASTA format 
    0001 MKAAAKTQKP KRQEEHANFI SWRFALLCGC ILLALAFLLG RVAWLQVISP DMLVKEGDMR SLRVQQVSTS 
0071 RGMITDRSGR PLAVSVPVKA IWADPKEVHD AGGISVGDRW KALANALNIP LDQLSARINA NPKGRFIYLA 
0141 RQVNPDMADY IKKLKLPGIH LREESRRYYP SGEVTAHLIG FTNVDSQGIE GVEKSFDKWL TGQPGERIVR 
0211 KDRYGRVIED ISSTDSQAAH NLALSIDERL QALVYRELNN AVAFNKAESG SAVLVDVNTG EVLAMANSPS 
0281 YNPNNLSGTP KEAMRNRTIT DVFEPGSTVK PMVVMTALQR GVVRENSVLN TIPYRINGHE IKDVARYSEL 
0351 TLTGVLQKSS NVGVSKLALA MPSSALVDTY SRFGLGKATN LGLVGERSGL YPQKQRWSDI ERATFSFGYG 
0421 LMVTPLQLAR VYATIGSYGI YRPLSITKVD PPVPGERVFP ESIVRTVVHM MESVALPGGG GVKAAIKGYR 
0491 IAIKTGTAKK VGPDGRYINK YIAYTAGVAP ASQPRFALVV VINDPQAGKY YGGAVSAPVF GAIMGGVLRT 
0561 MNIEPDALTT GDKNEFVINQ GEGTGGRS
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             TAGCCGGGTG GAAAGGATCG CCACGGAAAA 
-070 GCTGCAAATG CAGCATGTTG ATCCGTCACA AGAAAATATC GTAGTGCAAA AATAAGGATA AACGCGACGC 
0001 atgaaagcag cggcgaaaac gcagaaacca aaacgtcagg aagaacatgc caactttatc agttggcgtt 
0071 ttgcgttgtt atgcggctgt attctcctgg cgctggcttt tctgctcgga cgcgtagcgt ggttacaagt 
0141 tatctccccg gatatgctgg tgaaagaggg cgacatgcgt tctcttcgcg ttcagcaagt ttccacctcc 
0211 cgcggcatga ttactgaccg ttctggtcgc ccgttagcgg tgagcgtgcc ggtaaaagcg atttgggctg 
0281 acccgaaaga agtgcatgac gctggcggta tcagcgtcgg tgaccgctgg aaggcgctgg ctaacgcgct 
0351 caatattccg ctggatcagc tttcagcccg cattaacgcc aacccgaaag ggcgctttat ttatctggcg 
0421 cgtcaggtga accctgacat ggcggactac atcaaaaaac tgaaactgcc ggggattcat ctgcgtgaag 
0491 agtctcgccg ttactatccg tccggcgaag tgactgctca cctcatcggc tttactaacg tcgatagtca 
0561 agggattgag ggcgttgaga agagtttcga taaatggctt accgggcagc cgggtgagcg cattgtgcgt 
0631 aaagaccgct atggtcgcgt aattgaagat atttcttcta ctgacagcca ggcagcgcac aacctggcgc 
0701 tgagtattga tgaacgcctg caggcgctgg tttatcgcga actgaacaac gcggtggcct ttaacaaggc 
0771 tgaatctggt agcgccgtgc tggtggatgt caacaccggt gaagtgctgg cgatggctaa cagcccgtca 
0841 tacaacccta acaatctgag cggcacgccg aaagaggcga tgcgtaaccg taccatcacc gacgtgtttg 
0911 aaccgggctc aacggttaaa ccgatggtgg taatgaccgc gttgcaacgt ggcgtggtgc gggaaaactc 
0981 ggtactcaat accattcctt atcgaattaa cggccacgaa atcaaagacg tggcacgcta cagcgaatta 
1051 accctgaccg gggtattaca gaagtcgagt aacgtcggtg tttccaagct ggcgttagcg atgccgtcct 
1121 cagcgttagt agatacttac tcacgttttg gactgggaaa agcgaccaat ttggggttgg tcggagaacg 
1191 cagtggctta tatcctcaaa aacaacggtg gtctgacata gagagggcca ccttctcttt cggctacggg 
1261 ctaatggtaa caccattaca gttagcgcga gtctacgcaa ctatcggcag ctacggcatt tatcgcccac 
1331 tgtcgattac caaagttgac cccccggttc ccggtgaacg tgtcttcccg gaatccattg tccgcactgt 
1401 ggtgcatatg atggaaagcg tggcgctacc aggcggcggc ggcgtgaagg cggcgattaa aggctatcgt 
1471 atcgccatta aaaccggtac cgcgaaaaag gtcgggccgg acggtcgcta catcaataaa tatattgctt 
1541 ataccgcagg cgttgcgcct gcgagtcagc cgcgcttcgc gctggttgtt gttatcaacg atccgcaggc 
1611 gggtaaatac tacggcggcg ccgtttccgc gccggtcttt ggtgccatca tgggcggcgt attgcgtacc 
1681 atgaacatcg agccggatgc gctgacaacg ggcgataaaa atgaatttgt gattaatcaa ggcgagggga 
1751 caggtggcag atcgtaaTTT GCGCGACCTT CTTGCTCCGT GGGTGCCAGA CGCACCTTCG CGAGCACTGC 
1821 GAGAGATGAC ACTCGACAGC CGTGTGGCTG CGGCGGGCGA TCTCTTT