PEC Original Annotations
|
Essentiality
|
Class
|
non-essential
|
References (PMID)
|
|
Deletion
|
DE(argA-thyA)232
,
OCL44,45-11
(D)
,
OCL44,45-15 (AK1-9 / BK2-9)
(D)
,
OCL44,45-3
(D)
|
Related gene (W3110 PEC)
|
Gene Search
|
Search MG1655 PEC by gene name:
recB
|
Related strains
|
Strains Search
|
Search strains by gene name:
recB
Search strains by all related name:
recB b2820 ECK2816 ior JW2788 rorA
|
General information (Go to Linear View:)
|
Gene Name
|
recB
|
Alternative name
|
b2820,ECK2816,ior,JW2788,rorA
|
Location, Length
|
2,950,483 - 2,954,025
(
-
)
;
63.59
min
;
3543
(bp) ,
1180
(aa)
|
Product
|
exonuclease V (RecBCD complex), beta subunit
|
Operon Name
|
ptrA-recBD
|
Note
|
DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease; GO_process: GO:0006308 - DNA catabolic process; GO_process: GO:0006310 - DNA recombination
|
Function
|
enzyme; Degradation of DNA
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( recB )
GO:0003677
;
DNA binding ( recB )
GO:0004003
;
ATP-dependent DNA helicase activity ( recB )
GO:0004386
;
helicase activity ( recB )
GO:0004518
;
nuclease activity ( recB )
GO:0004519
;
endonuclease activity ( recB )
GO:0004527
;
exonuclease activity ( recB )
GO:0005524
;
ATP binding ( recB )
GO:0006281
;
DNA repair ( recB )
GO:0006974
;
response to DNA damage stimulus ( recB )
GO:0008854
;
exodeoxyribonuclease V activity ( recB )
GO:0016787
;
hydrolase activity ( recB )
|
PID
|
1789183
|
EC number
(KEGG Pathway)
|
3.1.11.5
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
EcoGene
|
recB
(
EG10824
)
|
Number of removed
|
1 aa cleaved
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
EX5B_ECOLI
(
P08394
)
|
- Protein name
|
Exodeoxyribonuclease V beta chain
|
- Synonyms
|
EC 3.1.11.5, Exodeoxyribonuclease V 135 kDa polypeptide
|
- Gene name
|
Name=recB; Synonyms=rorA; OrderedLocusNames=b2820;
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- Comments
|
FUNCTION:Required for efficient DNA repair; it catalyzes the unwinding of double-stranded DNA and the cleavage of single-stranded DNA and it stimulates local genetic recombination. All of these activities require concomitant hydrolysis of ATP. CATALYTIC ACTIVITY:Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides. SUBUNIT:Consist of three subunits; recB, recC and recD. SIMILARITY:Belongs to the helicase family. UvrD subfamily.
|
Entry name(Acc.no)
|
Q8FEB3
(
Q8FEB3
)
|
- Protein name
|
Exodeoxyribonuclease V beta chain
|
- Synonyms
|
EC 3.1.11.5
|
- Gene name
|
Name=recB; OrderedLocusNames=c3414;
|
- Comments
|
SIMILARITY:Belongs to the ATP-dependent AMP-binding enzyme family.
|
Entry name(Acc.no)
|
Q8X6M9
(
Q8X6M9
)
|
- Protein name
|
DNA helicase, ATP-dependent dsDNA/ssDNA exonuclease V subunit, ssDNA endonuclease.
|
- Synonyms
|
|
- Gene name
|
Name=recB; OrderedLocusNames=z4137;
|
- Comments
|
SIMILARITY:Belongs to the beta-ketoacyl-ACP synthases family.
|
Homology Analysis
|
BLAST
|
Bacteria
|
GTOP
|
recB
(
homologous genes of other bacterias
)
|
PDB
(database updated :
2007.02.20
)
|
PSI-BLAST
|
Chain B, Recbcd:dna Complex
|
SWISS-PROT
(database updated :
2007.02.20
)
|
BLAST
|
Exodeoxyribonuclease V beta chain
|
PSI-BLAST
|
Exodeoxyribonuclease V beta chain
|
nr
(database updated :
2007.02.20
)
|
BLAST
|
exonuclease V (RecBCD complex), beta subunit [Escherichia coli K12]
|
Pfam 28.0
(database updated :
2015-05
)
|
Pfam
|
|
PROSITE
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
RGD
ATP_GTP_A
CRYSTALLIN_BETAGAMMA
|
MMBR References
|
J Bacteriol. 1984;157(1):21-7.
Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12.
Dykstra CC, Prasher D, Kushner SR.
(
6317651
)
|
Mol Gen Genet. 1995;246(4):514-8.
An Escherichia coli strain deficient for both exonuclease V and deoxycytidine triphosphate deaminase shows enhanced sensitivity to ionizing radiation.
Estevenon AM, Kooistra J, Sicard N.
(
7891665
)
|
Nucleic Acids Res. 1986;14:8573-8582
Complete nucleotide sequence of the Escherichia coli recB gene.
Finch, P. W., A. Storey, K. E. Chapman, K. Brown, I. D. Hickson, and P. T. Emmerson.
|
Nature. 1981;294(5841):578-80.
Identification of the Escherichia coli recB and recC gene products.
Hickson ID, Emmerson PT.
(
6458768
)
|
Biochem Biophys Res Commun. 1982;109(2):414-22.
Fine structure of the recB and recC gene region of Escherichia coli.
Sasaki M, Fujiyoshi T, Shimada K, Takagi Y.
(
6295393
)
|
J Biol Chem. 1992;267:4981-4987
The RecB subunit of the Escherichia coli RecBCD enzyme couples ATP hydrolysis to DNA unwinding.
Boehmer, P. E., and P. T. Emmerson.
|
J Biol Chem. 1995;270:24451-24458
Monomeric RecBCD enzyme binds and unwinds DNA.
Taylor, A. F., and G. R. Smith.
|
J Biol Chem. 1971;246:6543-6555
The isolation and characterization of an adenosine triphosphate-dependent deoxyribonuclease directed by recB, C genes.
Wright, M., G. Bultin, and J. Hurwitz.
|
Mol Gen Genet. 1985;201(3):525-8.
Role of Escherichia coli RecBC enzyme in SOS induction.
Chaudhury AM, Smith GR.
(
3911029
)
|
Biochimie. 1991;73(4):523-32.
rec genes and homologous recombination proteins in Escherichia coli.
Clark AJ.
(
1911953
)
|
Sequences |
Amino acid
FASTA format
|
0001 MSDVAETLDP LRLPLQGERL IEASAGTGKT FTIAALYLRL LLGLGGSAAF PRPLTVEELL VVTFTEAATA
0071 ELRGRIRSNI HELRIACLRE TTDNPLYERL LEEIDDKAQA AQWLLLAERQ MDEAAVFTIH GFCQRMLNLN
0141 AFESGMLFEQ QLIEDESLLR YQACADFWRR HCYPLPREIA QVVFETWKGP QALLRDINRY LQGEAPVIKA
0211 PPPDDETLAS RHAQIVARID TVKQQWRDAV GELDALIESS GIDRRKFNRS NQAKWIDKIS AWAEEETNSY
0281 QLPESLEKFS QRFLEDRTKA GGETPRHPLF EAIDQLLAEP LSIRDLVITR ALAEIRETVA REKRRRGELG
0351 FDDMLSRLDS ALRSESGEVL AAAIRTRFPV AMIDEFQDTD PQQYRIFRRI WHHQPETALL LIGDPKQAIY
0421 AFRGADIFTY MKARSEVHAH YTLDTNWRSA PGMVNSVNKL FSQTDDAFMF REIPFIPVKS AGKNQALRFV
0491 FKGETQPAMK MWLMEGESCG VGDYQSTMAQ VCAAQIRDWL QAGQRGEALL MNGDDARPVR ASDISVLVRS
0561 RQEAAQVRDA LTLLEIPSVY LSNRDSVFET LEAQEMLWLL QAVMTPEREN TLRSALATSM MGLNALDIET
0631 LNNDEHAWDV VVEEFDGYRQ IWRKRGVMPM LRALMSARNI AENLLATAGG ERRLTDILHI SELLQEAGTQ
0701 LESEHALVRW LSQHILEPDS NASSQQMRLE SDKHLVQIVT IHKSKGLEYP LVWLPFITNF RVQEQAFYHD
0771 RHSFEAVLDL NAAPESVDLA EAERLAEDLR LLYVALTRSV WHCSLGVAPL VRRRGDKKGD TDVHQSALGR
0841 LLQKGEPQDA AGLRTCIEAL CDDDIAWQTA QTGDNQPWQV NDVSTAELNA KTLQRLPGDN WRVTSYSGLQ
0911 QRGHGIAQDL MPRLDVDAAG VASVVEEPTL TPHQFPRGAS PGTFLHSLFE DLDFTQPVDP NWVREKLELG
0981 GFESQWEPVL TEWITAVLQA PLNETGVSLS QLSARNKQVE MEFYLPISEP LIASQLDTLI RQFDPLSAGC
1051 PPLEFMQVRG MLKGFIDLVF RHEGRYYLLD YKSNWLGEDS SAYTQQAMAA AMQAHRYDLQ YQLYTLALHR
1121 YLRHRIADYD YEHHFGGVIY LFLRGVDKEH PQQGIYTTRP NAGLIALMDE MFAGMTLEEA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 agccagaacg ggaaagccga atatgtacac
-070 cctgaaggct ggaaagtgtg ggagaacgtc agcgcgttgc agcaaacaat gcccctgatg agtgaaaaga
0001 atgagtgatg tcgccgagac actagatcct ttgcgcttgc ccttacaggg tgagcgcctg attgaagcct
0071 ctgccggcac aggcaaaacc tttacgattg cggcgctcta tttgcgcctg ttacttggac taggcggttc
0141 cgccgccttt ccccgcccgc tgaccgttga agaactgctg gtggtcacct ttaccgaggc tgccacggca
0211 gaattgcgcg gtcgtatccg tagcaatatc cacgagttgc gcatcgcctg tctgcgtgaa accaccgaca
0281 atccactgta cgaacgcctg ctggaagaga tcgacgataa agcgcaagcc gcgcagtggt tgttgttagc
0351 cgaacggcag atggatgaag cggcagtctt tactattcac ggcttttgcc agcgcatgct caacctgaat
0421 gcctttgaat ccggcatgct gtttgagcag cagctgattg aagatgagtc tctgctacgc taccaggcct
0491 gcgccgattt ctggcgtcgc cactgctacc cgctgccgcg tgaaatagcc caggtcgtct ttgaaacctg
0561 gaaagggccg caggcgttgc tgcgcgatat taatcgttat ctgcaaggcg aagcgccggt tatcaaagca
0631 ccgccgcccg atgatgaaac gctggcttcc cgtcacgcgc aaattgtggc gcgtattgat acggtaaaac
0701 agcagtggcg cgacgcagtg ggtgaactgg atgcgctgat cgaatcttct ggtattgatc gacgcaagtt
0771 taaccgtagc aatcaggcta aatggatcga caagatcagc gcctgggcag aagaagagac aaacagttat
0841 cagttgccgg agtcgctgga aaaattctcc cagcgtttct tagaagatcg cacgaaggcc gggggggaaa
0911 ccccgcgaca tccactgttt gaggcgatcg atcaactgct tgcagaacca ttgtcgatcc gcgatctggt
0981 gatcacccgc gcattggctg agatccgcga aacagtagcg cgtgaaaaac gccgccgtgg cgaattgggt
1051 tttgatgaca tgttaagtcg gctcgattcc gcgctgcgta gcgaaagcgg tgaggtgttg gcagcggcga
1121 tccgtacgcg attcccggtg gcaatgatcg atgaatttca ggataccgac ccccagcagt accgaatttt
1191 tcgccgtatc tggcaccatc agccggaaac cgcattgttg ctaattggcg acccgaagca ggccatatat
1261 gcattccggg gtgcggatat cttcacttat atgaaggcgc gtagcgaagt tcacgcccac tacactttag
1331 acaccaactg gcgttccgca ccaggaatgg tgaacagcgt gaataagctt ttcagccaga ctgatgacgc
1401 gttcatgttt cgcgaaatac cgtttattcc agtgaaatca gccgggaaaa atcaggcgtt acgttttgta
1471 tttaaaggtg aaacacagcc tgcgatgaaa atgtggctga tggaaggcga aagctgcggc gttggcgatt
1541 atcaaagtac catggcgcag gtatgtgctg cgcaaatccg cgactggcta caagccggac agcggggcga
1611 agcgttgctg atgaacggcg acgacgcgcg tccggtgcgt gcttcggaca tcagtgtgct ggtgcgcagc
1681 cgccaggagg ccgcccaggt gcgcgatgcc ttaacgttgc tggaaatccc ttccgtttac ctttcgaacc
1751 gcgacagtgt ttttgaaact ctggaagcgc aggaaatgct ttggttgttg caggcggtga tgacgcccga
1821 acgtgagaac accctgcgta gtgcgctggc aacgtcaatg atggggctga acgcgctgga tatcgaaacg
1891 ctgaacaatg acgaacatgc gtgggatgtg gtagtcgaag agttcgatgg ttatcggcaa atctggcgca
1961 aacgtggcgt tatgccgatg ctgcgggcgc tgatgtcggc gcgtaacatt gctgaaaact tgctggcaac
2031 ggcaggcggt gagcggcgtc ttaccgatat cttgcatatc agcgaactgc tacaagaagc cggaacgcag
2101 ctggaaagtg aacatgcgct ggtacgctgg ttatcgcaac atatcctcga gccagacagt aatgcctcca
2171 gccaacaaat gcgtctcgaa agtgataaac atctggtgca gattgtcacg atccacaaat cgaaagggct
2241 ggaatatcca ttggtctggc tgccgtttat caccaatttc cgcgtccagg agcaggcgtt ttatcacgat
2311 cgccactcgt ttgaggcagt tctggatctt aatgctgcgc cagaaagcgt cgacctcgcg gaggccgaac
2381 gtctggcgga agatctgcgt ttgctttacg tggcgctgac acgttcggtt tggcattgca gtctcggcgt
2451 tgcaccgctg gtgcgccgtc gtggcgataa aaaaggtgac accgacgtcc accaaagtgc gctcgggcgt
2521 ttgctgcaaa aaggggaacc gcaagatgcg gcagggcttc gcacctgtat tgaagcgtta tgcgatgatg
2591 atattgcctg gcaaacggca caaactggtg ataaccaacc ctggcaggtt aatgatgttt ctacagcaga
2661 gctgaatgcg aagacgttac aacgattgcc cggcgataac tggcgcgtca ccagctactc tggtttgcaa
2731 cagcgtggtc acggtatcgc ccaggatttg atgcctcggc tggatgtcga tgctgcaggc gttgccagcg
2801 tcgttgaaga accgacgtta acaccacatc agtttccgcg cggtgcgtca ccggggacgt tcttgcacag
2871 tttgtttgaa gacctggatt ttacccagcc ggttgacccg aactgggtgc gggaaaaact ggaactcggc
2941 ggctttgaat cgcagtggga accggtattg accgagtgga tcacggctgt cctccaggca cctctcaatg
3011 aaaccggcgt aagcctgagt caactttccg cccgcaataa acaggtggag atggagtttt atctgccgat
3081 tagtgaaccg cttatcgcca gtcagcttga tacgttaatc cgccagtttg acccgctatc cgcaggctgc
3151 ccgccgctgg agttcatgca ggtacgtggc atgttaaaag gctttatcga cctggtgttc cgccacgaag
3221 ggcgttatta cctgctcgac tataaatcca actggttggg tgaagacagt tcggcttaca cccaacaggc
3291 tatggcagcg gcaatgcagg cacaccgcta tgatctgcaa tatcagcttt ataccctggc gctgcatcgt
3361 tatctgcgcc atcgcattgc tgattacgac tatgagcacc actttggcgg cgttatttat ctgttcctgc
3431 gtggcgttga taaagaacat ccgcaacagg ggatttacac aacccgaccc aacgccgggt tgattgccct
3501 gatggatgag atgtttgccg gtatgaccct ggaggaggcg taatgaaatt gcaaaagcaa ttactggaag
3571 ctgtggagca caaacagcta cgcccgctgg atgtgcaatt tgccctgacc gtggcgggag atgaacatcc
3641 tgc
|