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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Deletion
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OCR33,34-1
(D)
,
OCR33,34-2
(D)
,
OCR33,35-4 (AK4-2 / BK5-2)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
tsf
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Related strains
|
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Strains Search
|
Search strains by gene name:
tsf
Search strains by all related name:
tsf b0170 ECK0169 JW0165
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General information (Go to Linear View: )
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Gene Name
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tsf
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Alternative name
|
b0170,ECK0169,JW0165
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Location, Length
|
190,857 - 191,708
(
+
)
;
4.11
min
;
852
(bp) ,
283
(aa)
|
|
Product
|
protein chain elongation factor EF-Ts
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Operon Name
|
tff-rpsB-tsf
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006412 - translation
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Function
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factor; Proteins - translation and modification
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Gene Ontology
|
GO:0003746
;
translation elongation factor activity ( tsf )
GO:0005515
;
protein binding ( tsf )
GO:0005622
;
intracellular ( tsf )
GO:0005624
;
membrane fraction ( tsf )
GO:0005737
;
cytoplasm ( tsf )
GO:0006412
;
translation ( tsf )
GO:0006414
;
translational elongation ( tsf )
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PID
|
1786366
|
EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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tsf
(
EG11033
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
|
EFTS_ECOLI
(
P02997
)
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- Protein name
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Elongation factor Ts
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- Synonyms
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EF-Ts
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- Gene name
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OrderedLocusNames=b0170, c0206, z0181, ECs0172, SF0160, S0163;
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- Comments
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FUNCTION:Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
SUBUNIT:Heterotetramer composed of two EF-Ts.EF-Tu dimer complex.
SUBCELLULAR LOCATION:Cytoplasmic.
MASS SPECTROMETRY:MW=30294; MW_ERR=6; METHOD=Electrospray; RANGE=1-282; NOTE=Ref.14.
SIMILARITY:Belongs to the EF-Ts family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
tsf
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain B, Elongation Factor Complex Ef-TuEF-Ts From Escherichia Coli
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|
SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Elongation factor Ts (EF-Ts)
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PSI-BLAST
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Elongation factor Ts (EF-Ts)
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nr
(database updated :
2007.02.20
)
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BLAST
|
elongation factor Ts [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
|
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
EF_TS_1
EF_TS_2
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MMBR References
|
Mol Gen Genet. 1981;182(1):178-9.
Revised location of the Escherichia coli gene coding for ribosomal protein S2.
Lathe R, Bollen A, Herzog R.
(
7022135
)
|
Mol Gen Genet. 1981;181(3):356-62.
Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed.
Bendiak DS, Friesen JD.
(
7017348
)
|
J Mol Biol. 1979;132(2):219-33.
A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB).
Bollen A, Lathe R, Herzog A, Denicourt D, Lecocq JP, Desmarez L, Lavalle R.
(
161328
)
|
Nucleic Acids Res. 1981;9:4163-4172
Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.
An, G., D. S. Bendiak, L. A. Mamelak, and J. D. Friesen.
|
| Sequences |
Amino acid
FASTA format
|
0001 MAEITASLVK ELRERTGAGM MDCKKALTEA NGDIELAIEN MRKSGAIKAA KKAGNVAADG VIKTKIDGNY
0071 GIILEVNCQT DFVAKDAGFQ AFADKVLDAA VAGKITDVEV LKAQFEEERV ALVAKIGENI NIRRVAALEG
0141 DVLGSYQHGA RIGVLVAAKG ADEELVKHIA MHVAASKPEF IKPEDVSAEV VEKEYQVQLD IAMQSGKPKE
0211 IAEKMVEGRM KKFTGEVSLT GQPFVMEPSK TVGQLLKEHN AEVTGFIRFE VGEGIEKVET DFAAEVAAMS
0281 KQS
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TAGTACACGT TTGGTTAGGG GGCCTGCATA
-070 TGGCCCCCTT TTTCACTTTT ATATCTGTGC GGTTTAATGC CGGGCAGATC ACATCTCCGA GGATTTTAGA
0001 atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg atggattgca
0071 aaaaagcact gactgaagct aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat
0141 taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac
0211 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
0281 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga
0351 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc gctggaaggc
0421 gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag
0491 agctggttaa acacatcgct atgcacgttg ctgcaagcaa gccagaattc atcaaaccgg aagacgtatc
0561 cgctgaagtg gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa
0631 atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
0701 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat
0771 ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc tgcgatgtcc
0841 aagcagtctt aaTTATCAAA AAGGAGCCGC CTGAGGGCGG CTTCTTTTTG TGCCCATCTT GTAAATTCAG
0911 CTAACCCTTG TGGGGCTGCG CTGAAAAGCG ACGTACAATG TC
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