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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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9-1
(LD)
,
OCR36,37-6 (LD 9C::Ap)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
lacI
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Related strains
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Strains Search
|
Search strains by gene name:
lacI
Search strains by all related name:
lacI b0345 ECK0342 JW0336
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General information (Go to Linear View: )
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Gene Name
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lacI
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Alternative name
|
b0345,ECK0342,JW0336
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Location, Length
|
365,652 - 366,734
(
-
)
;
7.88
min
;
1083
(bp) ,
360
(aa)
|
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Product
|
DNA-binding transcriptional repressor
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Operon Name
|
mhpR-lacI
|
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Note
|
transcriptional repressor of the lac operon; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0006350 - transcription
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Function
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regulator; Degradation of small molecules: Carbon compounds
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Gene Ontology
|
GO:0003677
;
DNA binding ( lacI )
GO:0003700
;
transcription factor activity ( lacI )
GO:0005515
;
protein binding ( lacI )
GO:0005622
;
intracellular ( lacI )
GO:0006350
;
transcription ( lacI )
GO:0006355
;
regulation of transcription, DNA-dependent ( lacI )
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PID
|
1786540
|
EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
lacI
(
EG10525
)
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Number of removed
|
0 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
LACI_ECOLI
(
P03023
;
O09196; P71309; Q47338
)
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- Protein name
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Lactose operon repressor.
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- Synonyms
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- Gene name
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Name=lacI; OrderedLocusNames=b0345;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
lacI
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Lac Repressor
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Lactose operon repressor
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PSI-BLAST
|
HTH-type transcriptional repressor purR
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nr
(database updated :
2007.02.20
)
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BLAST
|
lac repressor [Cloning vector pCMVLacI]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
CRYSTALLIN_BETAGAMMA
HTH_LACI_FAMILY
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MMBR References
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Nature. 1978;274(5673):765-9.
Sequence of the lacI gene.
Farabaugh PJ.
(
355891
)
|
J Mol Biol. 1988;200(2):239-51.
Missense mutation in the lacI gene of Escherichia coli. Inferences on the structure of the repressor protein.
Gordon AJ, Burns PA, Fix DF, Yatagai F, Allen FL, Horsfall MJ, Halliday JA, Gray J, Bernelot-Moens C, Glickman BW.
(
3286877
)
|
J Biol Chem. 1982;257:11740-11746
A termination site for Lac 1 transcription is between the CAP site and the lac promoter.
Horowitz, H., and T. Platt.
|
Biochim Biophys Acta. 1964;90:609-10.
A new method for the selection of mutants of escherichia coli forming beta-glactosidase cnstitutively.
Kesslar DP, Ricknberg HV.
(
14237870
)
|
Mol Gen Genet. 1969;105(1):74-83.
Mutations conferring quantitative and qualitative increases in beta-galactosidase activity in Escherichia coli.
Langridge J.
(
4899399
)
|
Mol Gen Genet. 1978;164(2):163-9.
Analysis of the regulatory mechanisms controlling the synthesis of the hexitol transport systems in Escherichia coli K12.
Lengeler J, Steinberger H.
(
360044
)
|
J Bacteriol. 1957;73(4):544-52.
Genetic control of raffinose utilization in Escherichia coli.
Lester G, Bonner DM.
(
13428689
)
|
Biochem Biophys Res Commun. 1965;20(2):230-4.
Genetic control of catabolite repression of the lac operon in Escherichia coli.
Loomis WF Jr, Magasanik B.
(
5321900
)
|
J Mol Biol. 1967;23(3):487-94.
The catabolite repression gene of the lac operon in Escherichia coli.
Loomis WF Jr, Magasanik B.
(
5340246
)
|
J Mol Biol. 1994;240(5):421-33.
Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence.
Markiewicz P, Kleina LG, Cruz C, Ehret S, Miller JH.
(
8046748
)
|
Proc Natl Acad Sci U S A. 1968;59(4):1259-64.
Mutants that mke more lac repressor.
Muller-Hill B, Crapo L, Gilbert W.
(
4870861
)
|
J Bacteriol. 1977;130(1):100-6.
Pseudoreversion of lactose operator-constitutive mutants.
Norwood WI, Sadler JR.
(
323217
)
|
Gene. 1986;42(3):283-92.
Cloning and characterization of several dominant-negative and tight-binding mutants of lac repressor.
Betz JL.
(
3732806
)
|
Eur J Biochem. 1975;59:491-509
Amino-acid sequence of lac repressor from Escherichia coli. Isolation, sequence analysis and sequence assembly of tryptic peptides and cyanogen-bromide fragments.
Beyreuther, K., K. Adler, E. Fanning, C. Murray, A. Klemm, and N. Geisler.
|
J Bacteriol. 1978;133(3):1358-67.
Permease-specific mutations in Salmonella typhimurium and Escherichia coli that release the glycerol, maltose, melibiose, and lactose transport systems from regulation by the phosphoenolpyruvate:sugar phosphotransferase system.
Saier MH Jr, Straud H, Massman LS, Judice JJ, Newman MJ, Feucht BU.
(
346569
)
|
J Mol Biol. 1971;59(2):273-305.
The nature of lactose operator constitive mutations.
Smith TF, Sadler JR.
(
4935786
)
|
Mol Gen Genet. 1980;178(1):225-7.
DNA sequence alteration resulting from a mutation impairing promoter function in the lac repressor gene.
Calos MP, Miller JH.
(
6770231
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS
0071 LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC
0141 TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI
0211 QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS
0281 CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA
0351 RQVSRLESGQ
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Nucleotide
FASTA format
View sequence in neighbor 100bp
|
0001 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
0071 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
0141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
0211 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
0281 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
0351 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
0421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
0491 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
0561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
0631 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
0701 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
0771 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
0841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
0911 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
0981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
1051 cgacaggttt cccgactgga aagcgggcag tga
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