|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
2-2
(LD)
,
OCL09,11-1 (LD 2-2'_CRS)
(D)
,
OCL09,11-2 (LD 2-2'_P/O)
(D)
,
OCL10
(D)
,
OCL11
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
malS
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
malS
Search strains by all related name:
malS b3571 ECK3560 JW3543
|
General information (Go to Linear View: )
|
|
Gene Name
|
malS
|
|
Alternative name
|
b3571,ECK3560,JW3543
|
|
Location, Length
|
3,735,520 - 3,737,550
(
+
)
;
80.51
min
;
2031
(bp) ,
676
(aa)
|
|
Product
|
alpha-amylase
|
|
Operon Name
|
malS
|
|
Note
|
GO_component: GO:0042597 - periplasmic space; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0000272 - polysaccharide catabolic process; GO_process: GO:0006508 - proteolysis
|
|
Function
|
enzyme; Degradation of polysaccharides
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( malS )
GO:0004556
;
alpha-amylase activity ( malS )
GO:0005509
;
calcium ion binding ( malS )
GO:0005975
;
carbohydrate metabolic process ( malS )
GO:0008152
;
metabolic process ( malS )
GO:0016787
;
hydrolase activity ( malS )
GO:0016798
;
hydrolase activity, acting on glycosyl bonds ( malS )
GO:0042597
;
periplasmic space ( malS )
GO:0043169
;
cation binding ( malS )
GO:0046872
;
metal ion binding ( malS )
|
|
PID
|
1789995
|
EC number
(KEGG Pathway)
|
3.2.1.1
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
malS
(
EG11316
)
|
|
Number of removed
|
17 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
AMY1_ECOLI
(
P25718
)
|
|
- Protein name
|
Alpha-amylase precursor
|
|
- Synonyms
|
EC 3.2.1.1, 1,4-alpha-D-glucan glucanohydrolase
|
|
- Gene name
|
Name=malS; OrderedLocusNames=b3571;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
malS
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain , Cyclomaltodextrin Glucanotransferase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Alpha-amylase precursor
|
|
PSI-BLAST
|
Alpha-amylase precursor
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
periplasmic alpha-amylase precursor [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Biol Chem. 1986;261:2946-2953
Alpha-amylase of Escherichia coli, mapping and cloning of the structural gene, malS, and identification of its product as a periplasmic protein.
Freundlieb, S., and W. Boos.
|
J Biol Chem. 1992;267:5148-5154
Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by MalS.
Schneider, E., S. Freundlieb, S. Tapio, and W. Boos.
|
| Sequences |
Amino acid
FASTA format
|
0001 MKLAACFLTL LPGFAVAASW TSPGFPAFSE QGTGTFVSHA QLPKGTRPLT LNFDQQCWQP ADAIKLNQML
0071 SLQPCSNTPP QWRLFRDGEY TLQIDTRSGT PTLMISIQNA AEPVASLVRE CPKWDGLPLT VDVSATFPEG
0141 AAVRDYYSQQ IAIVKNGQIM LQPAATSNGL LLLERAETDT SAPFDWHNAT VYFVLTDRFE NGDPSNDQSY
0211 GRHKDGMAEI GTFHGGDLRG LTNKLDYLQQ LGVNALWISA PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW
0281 TNLDANMGNE ADLRTLVDSA HQRGIRILFD VVMNHTGYAT LADMQEYQFG ALYLSGDEVK KSLGERWSDW
0351 KPAAGQTWHS FNDYINFSDK TGWDKWWGKN WIRTDIGDYD NPGFDDLTMS LAFLPDIKTE STTASGLPVF
0421 YKNKMDTHAK AIDGYTPRDY LTHWLSQWVR DYGIDGFRVD TAKHVELPAW QQLKTEASAA LREWKKANPD
0491 KALDDKPFWM TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAQ MDTTWQQMAE KLQGFNVLSY
0561 LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESSRPFG PTGSDPLQGT RSDMNWQDVS GKSAASVAHW
0631 QKISQFRARH PAIGAGKQTT LLLKQGYGFV REHGDDKVLV VWAGQQ
|
Nucleotide
FASTA format
View sequence in neighbor 100bp
|
0001 atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg
0071 ggtttcccgc ctttagcgaa caggggacag gaacatttgt cagccacgcg cagttgccca aaggtacgcg
0141 tccactaacg ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa tcagatgctt
0211 tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga cggcgaatat acgctgcaaa
0281 tagacacccg ctccggtacg ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaagcct
0351 ggtccgtgaa tgcccgaaat gggatggatt accgctcaca gtggatgtca gcgccacttt cccggaagga
0421 gccgccgtac gggattatta cagccagcaa attgcgatag tgaagaacgg tcaaataatg ttacaacccg
0491 ctgccaccag caacggttta ctcctgctgg aacgggcaga aactgacaca tccgcccctt tcgactggca
0561 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga ccagagttac
0631 ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga tttacgcggc ctgaccaaca
0701 aactggatta cctccagcag ttgggcgtta atgctttatg gataagcgcc ccatttgagc aaattcacgg
0771 ctgggtcggc ggcggtacaa aaggcgattt cccgcattat gcctaccacg gttattacac acaggactgg
0841 acgaatcttg atgccaatat gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg
0911 gtattcgtat tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
0981 tcagtttggc gcgttatatc tttctggtga cgaagtgaaa aaatcgctgg gtgaacgctg gagcgactgg
1051 aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt cagcgacaaa acaggctggg
1121 ataaatggtg gggaaaaaac tggatcagaa cggatatcgg cgattacgac aatcctggat tcgacgatct
1191 cactatgtcg ctagcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc
1261 tataaaaaca aaatggatac ccacgccaaa gccattgacg gctatacgcc gcgcgattac ttaacccact
1331 ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
1401 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc taaccccgac
1471 aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca cggcgtgatg caaagtgact
1541 actatcgcca cggcttcgat gcgatgatca atttcgatta tcaggagcag gcggcgaaag cagtcgactg
1611 tctggcgcag atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac
1681 ctctcgtcgc atgatacccg cctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc
1751 caggcgcggt acaaatcttt tatggtgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
1821 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccagcgt cgcgcactgg
1891 cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa acaaacgaca cttttgctga
1961 agcagggcta cggctttgtt cgtgagcatg gcgacgataa agtgctggtc gtctgggcag ggcaacagta
2031 a
|
