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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCL22-6
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
rpsG
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Related strains
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Strains Search
|
Search strains by gene name:
rpsG
Search strains by all related name:
rpsG b3341 ECK3328 f179 JW3303
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General information (Go to Linear View: )
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Gene Name
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rpsG
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Alternative name
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b3341,ECK3328,f179,JW3303
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Location, Length
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3,471,564 - 3,472,103
(
-
)
;
74.82
min
;
540
(bp) ,
179
(aa)
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Product
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30S ribosomal subunit protein S7
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Operon Name
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rpsLG-fusA-tufA
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Note
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30S ribosomal subunit protein S7, initiates assembly; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0003735 - structural constituent of ribosome; GO_process: GO:0006412 - translation
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Function
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structural component; Ribosomal proteins - synthesis, modification
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Gene Ontology
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GO:0000049
;
tRNA binding ( rpsG )
GO:0003723
;
RNA binding ( rpsG )
GO:0003735
;
structural constituent of ribosome ( rpsG )
GO:0005622
;
intracellular ( rpsG )
GO:0005840
;
ribosome ( rpsG )
GO:0006412
;
translation ( rpsG )
GO:0015935
;
small ribosomal subunit ( rpsG )
GO:0019843
;
rRNA binding ( rpsG )
GO:0030529
;
ribonucleoprotein complex ( rpsG )
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PID
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1789739
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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rpsG
(
EG10906
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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RS7_ECOLI
(
P02359
)
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- Protein name
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30S ribosomal protein S7.
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- Synonyms
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- Gene name
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Name=rpsG; OrderedLocusNames=b3341;
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- Comments
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FUNCTION:One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.
FUNCTION:Protein S7 is also a translational repressor protein; it regulates the expression of the str operon members to different degrees by binding to its mRNA.
SUBUNIT:Part of the 30S ribosomal subunit. Contacts proteins S9 and S11 (By similarity). Cross-links to IF3 and the P and E site tRNAs.
MASS SPECTROMETRY:MW=19888.7; METHOD=MALDI; RANGE=1-178; NOTE=Ref.16.
MISCELLANEOUS:The strain K12 sequence is shown.
MISCELLANEOUS:Has been predicted to contact the N-terminal domain of IF-3 based on footprint studies (PubMed 11684020); exactly how IF-3 interacts with the 30S subunit is controversial.
SIMILARITY:Belongs to the S7P family of ribosomal proteins.
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Entry name(Acc.no)
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RS7_ECOL6
(
Q8X887
)
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- Protein name
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30S ribosomal protein S7.
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- Synonyms
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- Gene name
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Name=rpsG; OrderedLocusNames=c4114, z4699, ECs4192, SF3359, S4403;
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- Comments
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FUNCTION:One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA (By similarity).
SUBUNIT:Part of the 30S ribosomal subunit. Contacts proteins S9 and S11 (By similarity).
SIMILARITY:Belongs to the S7P family of ribosomal proteins.
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MMBR References
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J Biol Chem. 1980;255:4660-4666
DNA sequences from the str operon of Escherichia coli.
Post, L., and M. Nomura.
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| Sequences |
Amino acid
FASTA format
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0001 MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE AFEVALENVR
0071 PTVEVKSRRV GGSTYQVPVE VRPVRRNALA MRWIVEAARK RGDKSMALRL ANELSDAAEN KGTAVKKRED
0141 VHRMAEANKA FAHYRWLSLR SFSHQAGASS KQPALGYLN
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttaatggttc tccgttaagt aaggccaaac
-070 gttttaactt aaatgtcaaa ctaaactcgt agagttttgg acaatcctga attaacaacg gagtatttcc
0001 atgccacgtc gtcgcgtcat tggtcagcgt aaaattctgc cggatccgaa gttcggatca gaactgctgg
0071 ctaaatttgt aaatatcctg atggtagatg gtaaaaaatc tactgctgaa tctatcgtat acagcgcgct
0141 ggagaccctg gctcagcgct ctggtaaatc tgaactggaa gcattcgaag tagctctcga aaacgtgcgc
0211 ccgactgtag aagttaagtc tcgccgcgtt ggtggttcta cttatcaggt accagttgaa gtccgtccgg
0281 ttcgtcgtaa tgctctggca atgcgttgga tcgttgaagc tgctcgtaaa cgcggtgata aatccatggc
0351 tctgcgcctg gcgaacgaac tttctgatgc tgcagaaaac aaaggtactg cagttaagaa acgtgaagac
0421 gttcaccgta tggccgaagc caacaaggcg ttcgcacact accgttggtt atcccttcgg agttttagtc
0491 accaggcggg cgcttccagt aagcagcccg ctttgggcta cttaaattga acgcctaaaa gataaacgag
0561 gaaacaaatg gctcgtacaa cacccatcgc acgctaccgt aacatcggta tcagtgcgca catcgacgcc
0631 ggtaaaacca
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