MG1655
W3110
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Gene Report : lgt
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1989;171(1):565-8.
Identification and genetic mapping of the structural gene for an essential Escherichia coli membrane protein.
Williams MG, Fortson M, Dykstra CC, Jensen P, Kushner SR. ( 2644208 )
     Deletion OCL44,45-11 (D)  ,  OCL44,45-15 (AK1-9 / BK2-9) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lgt
Related strains
     Strains Search Search strains by gene name:  lgt   Search strains by all related name:  lgt b2828 ECK2824 JW2796 umpA

General information  (Go to Linear View:)
 Gene Name lgt  
 Alternative name b2828,ECK2824,JW2796,umpA  
 Location, Length 2,963,184 - 2,964,059 (  -  ) ;   63.87 min ; 876 (bp) ,   291 (aa) Go to Linear View
 Product phosphatidylglycerol-prolipoprotein diacylglyceryl transferase  
 Operon Name lgt-thyA  
 Note phosphatidylglycerol-prolipoprotein diacylglyceryl transferase; a major membrane phospholipid; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0008654 - phospholipid biosynthetic process; GO_process: GO:0006464 - protein modification process  
 Function enzyme; Macromolecule synthesis, modification: Phospholipids  
 Gene Ontology GO:0005886 ; plasma membrane ( lgt )
GO:0008961 ; phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity ( lgt )
GO:0009249 ; protein lipoylation ( lgt )
GO:0016020 ; membrane ( lgt )
GO:0016021 ; integral to membrane ( lgt )
GO:0016740 ; transferase activity ( lgt )
GO:0042158 ; lipoprotein biosynthetic process ( lgt )
 PID 1789192  
 EC number
  (KEGG Pathway)   		2.4.99.-  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) LGT_ECOLI ( P60955 ; P37149 )
    -  Protein name Prolipoprotein diacylglyceryl transferase  
    -  Synonyms EC 2.4.99.-  
    -  Gene name Name=lgt; Synonyms=umpA; OrderedLocusNames=b2828;  
    -  Comments
  • FUNCTION:Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins.
  • PATHWAY:Lipoprotein biosynthesis; first step.
  • SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane (Probable).
  • SIMILARITY:Belongs to the lgt family.  
    •   botton Entry name(Acc.no) LGT_ECO57 ( P60956 ; P37149 )
        -  Protein name Prolipoprotein diacylglyceryl transferase  
        -  Synonyms EC 2.4.99.-  
        -  Gene name Name=lgt; Synonyms=umpA; OrderedLocusNames=z4145, ECs3685;  
        -  Comments
  • FUNCTION:Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins (By similarity).
  • PATHWAY:Lipoprotein biosynthesis; first step.
  • SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane (Probable).
  • SIMILARITY:Belongs to the lgt family.  
    •   botton Entry name(Acc.no) LGT_ECOL6 ( P60957 ; P37149 )
        -  Protein name Prolipoprotein diacylglyceryl transferase  
        -  Synonyms EC 2.4.99.-  
        -  Gene name Name=lgt; Synonyms=umpA; OrderedLocusNames=c3423;  
        -  Comments
  • FUNCTION:Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins (By similarity).
  • PATHWAY:Lipoprotein biosynthesis; first step.
  • SUBCELLULAR LOCATION:Integral membrane protein. Inner membrane (Probable).
  • SIMILARITY:Belongs to the lgt family.  

    •  Linear View (Whole Mode)
    View Location
       2950.0  –  2975.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP lgt (  homologous genes of other bacterias  )  
        PDB     (database updated : 2007.02.20 )
            PSI-BLAST  
        SWISS-PROT     (database updated : 2007.02.20 )
             BLAST Prolipoprotein diacylglyceryl transferase  
             PSI-BLAST Prolipoprotein diacylglyceryl transferase
        nr     (database updated : 2007.02.20 )
             BLAST prolipoprotein diacylglyceryl transferase [Escherichia coli O157:H7 EDL933]  
    Pfam 28.0   (database updated : 2015-05 )
        Pfam
    PROSITE
        PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    LGT     

    Other Cross-Reference
        EcoCyc lgt 

    MMBR References
    J Bacteriol. 1995;177:1879-1882
    The umpA gene of Escherichia coli encodes phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (lgt) and regulates thymidylate synthase levels through translational coupling.
    Gan, K., K. Sankaran, M. G. Williams, M. Aldea, K. E. Rudd, S. R. Kushner, and H. C. Wu.
    J Mol Biol. 1995;254(4):552-65.
    Regulation of codBA operon expression in Escherichia coli by UTP-dependent reiterative transcription and UTP-sensitive transcriptional start site switching.
    Qi F, Turnbough CL Jr. ( 7500333 )
    J Bacteriol. 1995;177:6820-6824
    Structure-function relationship of bacterial prolipoprotein diacylglyceryl transferase: functionally significant conserved regions.
    Qi, H.-Y., K. Sankaran, K. Gan, and H. C. Wu.
    J Bacteriol. 1989;171(1):565-8.
    Identification and genetic mapping of the structural gene for an essential Escherichia coli membrane protein.
    Williams MG, Fortson M, Dykstra CC, Jensen P, Kushner SR. ( 2644208 )

    Sequences
    Amino acid
    FASTA format 
    0001 MTSSYLHFPE FDPVIFSIGP VALHWYGLMY LVGFIFAMWL ATRRANRPGS GWTKNEVENL LYAGFLGVFL 
0071 GGRIGYVLFY NFPQFMADPL YLFRVWDGGM SFHGGLIGVI VVMIIFARRT KRSFFQVSDF IAPLIPFGLG 
0141 AGRLGNFING ELWGRVDPNF PFAMLFPGSR TEDILLLQTN PQWQSIFDTY GVLPRHPSQL YELLLEGVVL 
0211 FIILNLYIRK PRPMGAVSGL FLIGYGAFRI IVEFFRQPDA QFTGAWVQYI SMGQILSIPM IVAGVIMMVW 
0281 AYRRSPQQHV S
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             ccttgtgcta ttattcgcac ctttggagcg 
-070 cctgaaacct gcggcgcgca tttcaatcgc tgttctcttt cagcgaaata acaagaactt gtggtgacag 
0001 atgaccagta gctatctgca ttttccggag tttgatccgg tcattttctc aataggaccc gtggcgcttc 
0071 actggtacgg cctgatgtat ctggtgggtt tcatttttgc aatgtggctg gcaacacgac gggcgaatcg 
0141 tccgggcagc ggctggacca aaaatgaagt tgaaaactta ctctatgcgg gcttcctcgg cgtcttcctc 
0211 gggggacgta ttggttatgt tctgttctac aatttcccgc agtttatggc cgatccgctg tatctgttcc 
0281 gtgtctggga cggcggcatg tctttccacg gcggcctgat tggcgttatc gtggtgatga ttatcttcgc 
0351 ccgccgtact aaacgttcct tcttccaggt ctctgatttt atcgcaccac tcattccgtt tggtcttggt 
0421 gccgggcgtc tgggcaactt tattaacggt gaattgtggg gccgcgttga cccgaacttc ccgtttgcca 
0491 tgctgttccc tggctcccgt acagaagata ttttgctgct gcaaaccaac ccgcagtggc aatccatttt 
0561 cgacacttac ggtgtgctgc cgcgccaccc atcacagctt tacgagctgc tgctggaagg tgtggtgctg 
0631 tttattatcc tcaacctgta tattcgtaaa ccacgcccaa tgggagctgt ctcaggtttg ttcctgattg 
0701 gttacggcgc gtttcgcatc attgttgagt ttttccgcca gcccgacgcg cagtttaccg gtgcctgggt 
0771 gcagtacatc agcatggggc aaattctttc catcccgatg attgtcgcgg gtgtgatcat gatggtctgg 
0841 gcatatcgtc gcagcccaca gcaacacgtt tcctgaggaa ccatgaaaca gtatttagaa ctgatgcaaa 
0911 aagtgctcga cgaaggcaca cagaaaaacg accgtaccgg aaccggaacg ctttccattt ttggtc