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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Mol Gen Genet. 1980;178(3):541-53.
A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaN, a new gene adjacent to the dnaA gene.
Sakakibara Y, Mizukami T.
(
6248733
)
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Deletion
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OCL04
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
dnaN
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Related strains
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Strains Search
|
Search strains by gene name:
dnaN
Search strains by all related name:
dnaN b3701 ECK3693 f366 JW3678
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General information (Go to Linear View: )
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Gene Name
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dnaN
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Alternative name
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b3701,ECK3693,f366,JW3678
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Location, Length
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3,879,244 - 3,880,344
(
-
)
;
83.61
min
;
1101
(bp) ,
366
(aa)
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Product
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DNA polymerase III, beta subunit
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Operon Name
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dnaAN-recF
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Note
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DNA polymerase III, beta-subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0003677
;
DNA binding ( dnaN )
GO:0003887
;
DNA-directed DNA polymerase activity ( dnaN )
GO:0005515
;
protein binding ( dnaN )
GO:0005737
;
cytoplasm ( dnaN )
GO:0006260
;
DNA replication ( dnaN )
GO:0008408
;
3'-5' exonuclease activity ( dnaN )
GO:0016740
;
transferase activity ( dnaN )
GO:0016779
;
nucleotidyltransferase activity ( dnaN )
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PID
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1790136
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EC number
(KEGG Pathway)
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2.7.7.7
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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dnaN
(
EG10242
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
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DP3B_ECOLI
(
P00583
)
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- Protein name
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DNA polymerase III, beta chain
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- Synonyms
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EC 2.7.7.7
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- Gene name
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Name=dnaN; OrderedLocusNames=b3701, c4623, z5192, ECs4636;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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dnaN
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Pol Iii (Beta Subunit) (E.C.2.7.7.7)
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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DNA polymerase III subunit beta
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PSI-BLAST
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DNA polymerase III subunit beta
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nr
(database updated :
2007.02.20
)
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BLAST
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DNA polymerase III subunit beta [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
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Gene. 1986;43(3):183-96.
Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences.
Armengod ME, Lambies E.
(
3527871
)
|
Nucleic Acids Res. 1982;10:7373-7385
The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
Hansen, E. B., F. G. Hansen, and K. von Meyenburg.
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Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M.
(
7574479
)
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J Bacteriol. 1979;140(3):825-34.
Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication.
Kimura M, Miki T, Hiraga S, Nagata T, Yura T.
(
160413
)
|
Gene. 1984;28(2):159-70.
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y.
(
6234204
)
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Plasmid. 1983;10(2):101-10.
Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome.
Ream LW, Clark AJ.
(
6356186
)
|
Mol Gen Genet. 1980;180(1):115-21.
Genetic and physical mapping of recF in Escherichia coli K-12.
Ream LW, Margossian L, Clark AJ, Hansen FG, von Meyenburg K.
(
6255290
)
|
Gene. 1981;13(1):47-55.
Organization and transcription of the dnaA and dnaN genes of Escherichia coli.
Sakakibara Y, Tsukano H, Sako T.
(
6453739
)
|
Mol Gen Genet. 1980;178(3):541-53.
A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaN, a new gene adjacent to the dnaA gene.
Sakakibara Y, Mizukami T.
(
6248733
)
|
Mol Gen Genet. 1980;179(3):521-6.
Coordinate expression of Escherichia coli dnaA and dnaN genes.
Sako T, Sakakibara Y.
(
6449652
)
|
Mol Gen Genet. 1980;180(2):267-73.
Identification of the dnaA and dnaN gene products of Escherichia coli.
Yuasa S, Sakakibara Y.
(
6258023
)
|
Proc Natl Acad Sci U S A. 1981;78:5391-5395
The dnaN gene codes for the subunit of DNA polymerase III holoenzyme of Escherichia coli.
Burgers, P. M. J., A. Kornberg, and Y. Sakakibara.
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| Sequences |
Amino acid
FASTA format
|
0001 MKFTVEREHL LKPLQQVSGP LGGRPTLPIL GNLLLQVADG TLSLTGTDLE MEMVARVALV QPHEPGATTV
0071 PARKFFDICR GLPEGAEIAV QLEGERMLVR SGRSRFSLST LPAADFPNLD DWQSEVEFTL PQATMKRLIE
0141 ATQFSMAHQD VRYYLNGMLF ETEGEELRTV ATDGHRLAVC SMPIGQSLPS HSVIVPRKGV IELMRMLDGG
0211 DNPLRVQIGS NNIRAHVGDF IFTSKLVDGR FPDYRRVLPK NPDKHLEAGC DLLKQAFARA AILSNEKFRG
0281 VRLYVSENQL KITANNPEQE EAEEILDVTY SGAEMEIGFN VSYVLDVLNA LKCENVRMML TDSVSSVQIE
0351 DAASQSAAYV VMPMRL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gtgcttcatg cctgccgtaa gatcgagcag
-070 ttgcgtgaag agagccacga tatcaaagaa gatttttcaa atttaatcag aacattgtca tcgtaaacct
0001 atgaaattta ccgtagaacg tgagcattta ttaaaaccgc tacaacaggt gagcggtccg ttaggtggtc
0071 gtcctacgct accgattctc ggtaatctgc tgttacaggt tgctgacggt acgttgtcgc tgaccggtac
0141 tgatctcgag atggaaatgg tggcacgtgt tgcgctggtt cagccacacg agccaggagc gacgaccgtt
0211 ccggcgcgca aattctttga tatctgccgt ggtctgcctg aaggcgcgga aattgccgtg cagctggaag
0281 gtgaacggat gctggtacgc tccgggcgta gccgtttttc gctgtctacc ctgccagcgg cggatttccc
0351 gaacctcgat gactggcaga gtgaagtcga atttaccctg ccgcaggcaa cgatgaagcg tctgattgaa
0421 gcgacccagt tttctatggc gcatcaggac gttcgctatt acttaaatgg tatgctgttt gaaaccgaag
0491 gtgaagaact gcgcaccgtg gcaaccgacg gccaccgtct ggcggtctgt tcaatgccaa ttggtcaatc
0561 tttgccaagc cattcggtga tcgtaccgcg taaaggcgtg attgaactga tgcgtatgct cgacggcggc
0631 gacaatccgc tgcgcgtaca gattggcagc aacaacattc gcgcccacgt tggcgacttt atcttcacct
0701 ccaaactggt ggatggtcgc ttcccggatt atcgccgcgt tctgccgaag aacccggaca aacatctgga
0771 agctggctgc gatctgctca agcaggcgtt tgctcgcgcg gcgattctct ctaacgagaa attccgcggc
0841 gtacgtcttt atgtcagcga aaaccagctg aaaatcaccg ccaacaaccc ggaacaggaa gaagcggaag
0911 agatcctcga cgttacctat agcggtgcgg agatggaaat cggcttcaac gtcagttatg tgctggatgt
0981 tctgaacgcg ctgaaatgcg aaaacgtccg catgatgctg accgattcgg tttccagcgt gcagattgaa
1051 gatgcggcca gccagagcgc ggcttatgtt gtcatgccaa tgagactgta atgtccctca cccgcttgtt
1121 gatccgcgat ttccgcaaca ttgaaaccgc ggatctcgcc ttatctcccg gctttaactt tctggtaggt
1191 gccaacggca g
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