MG1655
W3110
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Gene Report : carB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR28 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  carB
Related strains
     Strains Search Search strains by gene name:  carB   Search strains by all related name:  carB arg+ura b0033 cap ECK0034 JW0031 pyrA

General information  (Go to Linear View:)
 Gene Name carB  
 Alternative name arg+ura,b0033,cap,ECK0034,JW0031,pyrA  
 Location, Length 30,817 - 34,038 (  +  ) ;   0.66 min ; 3222 (bp) ,   1073 (aa) Go to Linear View
 Product carbamoyl-phosphate synthase large subunit  
 Operon Name carAB  
 Note GO_process: GO:0006526 - arginine biosynthetic process; GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process  
 Function enzyme; Pyrimidine ribonucleotide biosynthesis,1.5.1.3 metabolism; building block biosynthesis; amino acids; arginine,1.5.2.2 metabolism; building block biosynthesis; nucleotide; pyrimidine biosynthesis  
 Gene Ontology GO:0000166; nucleotide binding ( carB )
GO:0003824; catalytic activity ( carB )
GO:0004086; carbamoyl-phosphate synthase activity ( carB )
GO:0004088; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity ( carB )
GO:0005515; protein binding ( carB )
GO:0005524; ATP binding ( carB )
GO:0006221; pyrimidine nucleotide biosynthetic process ( carB )
GO:0006526; arginine biosynthetic process ( carB )
GO:0006807; nitrogen compound metabolic process ( carB )
GO:0008152; metabolic process ( carB )
GO:0008652; amino acid biosynthetic process ( carB )
GO:0016874; ligase activity ( carB )
GO:0030145; manganese ion binding ( carB )
GO:0046872; metal ion binding ( carB )
 PID 1786216  
 EC number
  (KEGG Pathway)
 
6.3.5.5  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene carB ( EG10135 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 4 more] )
  botton Entry name(Acc.no) CARA_ECOLI ( P00907 )
    -  Protein name Carbamoyl-phosphate synthase small chain  
    -  Synonyms EC 6.3.5.5, Carbamoyl- phosphate synthetase glutamine chain  
    -  Gene name Name=carA; Synonyms=pyrA; OrderedLocusNames=b0032, z0037, ECs0035;  

 Linear View (Whole Mode)
View Location
   40.817  –  65.817 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP carB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Carbamoyl Phosphate Synthetase From Escherichis Coli With Complexed With The Allosteric Ligand Imp  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Carbamoyl-phosphate synthase large chain  
         PSI-BLAST Carbamoyl-phosphate synthase large chain
    nr     (database updated : 2007.02.20 )
         BLAST carbamoyl-phosphate synthase large subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    PROKAR_LIPOPROTEIN    ATP_GTP_A    CPSASE_1    CPSASE_2     

Other Cross-Reference
    COG COG0458EF 
    EcoCyc carB 

MMBR References
J Bacteriol. 1980;143(2):914-20.
Patterns of polarity in the Escherichia coli car AB gene cluster.
Gigot D, Crabeel M, Feller A, Charlier D, Lissens W, Glansdorff N, Pierard A. ( 6162837 )
Proc Natl Acad Sci U S A. 1983;80:4629-4633
The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.
Nyunoya, H., and C. J. Lusty.
J Bacteriol. 1980;143(2):921-5.
Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.
Crabeel M, Charlier D, Weyens G, Feller A, Pierard A, Glansdorff N. ( 6451616 )

Sequences
Amino acid
FASTA format
0001 MPKRTDIKSI LILGAGPIVI GQACEFDYSG AQACKALREE GYRVILVNSN PATIMTDPEM ADATYIEPIH 
0071 WEVVRKIIEK ERPDAVLPTM GGQTALNCAL ELERQGVLEE FGVTMIGATA DAIDKAEDRR RFDVAMKKIG 
0141 LETARSGIAH TMEEALAVAA DVGFPCIIRP SFTMGGSGGG IAYNREEFEE ICARGLDLSP TKELLIDESL 
0211 IGWKEYEMEV VRDKNDNCII VCSIENFDAM GIHTGDSITV APAQTLTDKE YQIMRNASMA VLREIGVETG 
0281 GSNVQFAVNP KNGRLIVIEM NPRVSRSSAL ASKATGFPIA KVAAKLAVGY TLDELMNDIT GGRTPASFEP 
0351 SIDYVVTKIP RFNFEKFAGA NDRLTTQMKS VGEVMAIGRT QQESLQKALR GLEVGATGFD PKVSLDDPEA 
0421 LTKIRRELKD AGADRIWYIA DAFRAGLSVD GVFNLTNIDR WFLVQIEELV RLEEKVAEVG ITGLNADFLR 
0491 QLKRKGFADA RLAKLAGVRE AEIRKLRDQY DLHPVYKRVD TCAAEFATDT AYMYSTYEEE CEANPSTDRE 
0561 KIMVLGGGPN RIGQGIEFDY CCVHASLALR EDGYETIMVN CNPETVSTDY DTSDRLYFEP VTLEDVLEIV 
0631 RIEKPKGVIV QYGGQTPLKL ARALEAAGVP VIGTSPDAID RAEDRERFQH AVERLKLKQP ANATVTAIEM 
0701 AVEKAKEIGY PLVVRPSYVL GGRAMEIVYD EADLRRYFQT AVSVSNDAPV LLDHFLDDAV EVDVDAICDG 
0771 EMVLIGGIME HIEQAGVHSG DSACSLPAYT LSQEIQDVMR QQVQKLAFEL QVRGLMNVQF AVKNNEVYLI 
0841 EVNPRAARTV PFVSKATGVP LAKVAARVMA GKSLAEQGVT KEVIPPYYSV KEVVLPFNKF PGVDPLLGPE 
0911 MRSTGEVMGV GRTFAEAFAK AQLGSNSTMK KHGRALLSVR EGDKERVVDL AAKLLKQGFE LDATHGTAIV 
0981 LGEAGINPRL VNKVHEGRPH IQDRIKNGEY TYIINTTSGR RAIEDSRVIR RSALQYKVHY DTTLNGGFAT 
1051 AMALNADATE KVISVQEMHA QIK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CCAGCCCTGG TCCACACGAC GCCGCGCCGT 
-070 TGTTCGACCA CTTTATCGAG TTAATTGAGC AGTACCGTAA AACCGCTAAG TAATCAGGAG TAAAAGAGCC 
0001 atgccaaaac gtacagatat aaaaagtatc ctgattctgg gtgcgggccc gattgttatc ggtcaggcgt 
0071 gtgagtttga ctactctggc gcgcaagcgt gtaaagccct gcgtgaagag ggttaccgcg tcattctggt 
0141 gaactccaac ccggcgacca tcatgaccga cccggaaatg gctgatgcaa cctacatcga gccgattcac 
0211 tgggaagttg tacgcaagat tattgaaaaa gagcgcccgg acgcggtgct gccaacgatg ggcggtcaga 
0281 cggcgctgaa ctgcgcgctg gagctggaac gtcagggcgt gttggaagag ttcggtgtca ccatgattgg 
0351 tgccactgcc gatgcgattg ataaagcaga agaccgccgt cgtttcgacg tagcgatgaa gaaaattggt 
0421 ctggaaaccg cgcgttccgg tatcgcacac acgatggaag aagcgctggc ggttgccgct gacgtgggct 
0491 tcccgtgcat tattcgccca tcctttacca tgggcggtag cggcggcggt atcgcttata accgtgaaga 
0561 gtttgaagaa atttgcgccc gcggtctgga tctctctccg accaaagagt tgctgattga tgagtcgctg 
0631 atcggctgga aagagtacga gatggaagtg gtgcgtgata aaaacgacaa ctgcatcatc gtctgctcta 
0701 tcgaaaactt cgatgcgatg ggcatccaca ccggtgactc catcactgtc gcgccagccc aaacgctgac 
0771 cgacaaagaa tatcaaatca tgcgtaacgc ctcgatggcg gtgctgcgtg aaatcggcgt tgaaaccggt 
0841 ggttccaacg ttcagtttgc ggtgaacccg aaaaacggtc gtctgattgt tatcgaaatg aacccacgcg 
0911 tgtcccgttc ttcggcgctg gcgtcgaaag cgaccggttt cccgattgct aaagtggcgg cgaaactggc 
0981 ggtgggttac accctcgacg aactgatgaa cgacatcact ggcggacgta ctccggcctc cttcgagccg 
1051 tccatcgact atgtggttac taaaattcct cgcttcaact tcgaaaaatt cgccggtgct aacgaccgtc 
1121 tgaccactca gatgaaatcg gttggcgaag tgatggcgat tggtcgcacg cagcaggaat ccctgcaaaa 
1191 agcgctgcgc ggcctggaag tcggtgcgac tggattcgac ccgaaagtga gcctggatga cccggaagcg 
1261 ttaaccaaaa tccgtcgcga actgaaagac gcaggcgcag atcgtatctg gtacatcgcc gatgcgttcc 
1331 gtgcgggcct gtctgtggac ggcgtcttca acctgaccaa cattgaccgc tggttcctgg tacagattga 
1401 agagctggtg cgtctggaag agaaagtggc ggaagtgggc atcactggcc tgaacgctga cttcctgcgc 
1471 cagctgaaac gcaaaggctt tgccgatgcg cgcttggcaa aactggcggg cgtacgcgaa gcggaaatcc 
1541 gtaagctgcg tgaccagtat gacctgcacc cggtttataa gcgcgtggat acctgtgcgg cagagttcgc 
1611 caccgacacc gcttacatgt actccactta tgaagaagag tgcgaagcga atccgtctac cgaccgtgaa 
1681 aaaatcatgg tgcttggcgg cggcccgaac cgtatcggtc agggtatcga attcgactac tgttgcgtac 
1751 acgcctcgct ggcgctgcgc gaagacggtt acgaaaccat tatggttaac tgtaacccgg aaaccgtctc 
1821 caccgactac gacacttccg accgcctcta cttcgagccg gtaactctgg aagatgtgct ggaaatcgtg 
1891 cgtatcgaga agccgaaagg cgttatcgtc cagtacggcg gtcagacccc gctgaaactg gcgcgcgcgc 
1961 tggaagctgc tggcgtaccg gttatcggca ccagcccgga tgctatcgac cgtgcagaag accgtgaacg 
2031 cttccagcat gcggttgagc gtctgaaact gaaacaaccg gcgaacgcca ccgttaccgc tattgaaatg 
2101 gcggtagaga aggcgaaaga gattggctac ccgctggtgg tacgtccgtc ttacgttctc ggcggtcggg 
2171 cgatggaaat cgtctatgac gaagctgacc tgcgtcgcta cttccagacg gcggtcagcg tgtctaacga 
2241 tgcgccagtg ttgctggacc acttcctcga tgacgcggta gaagttgacg tggatgccat ctgcgacggc 
2311 gaaatggtgc tgattggcgg catcatggag catattgagc aggcgggcgt gcactccggt gactccgcat 
2381 gttctctgcc agcctacacc ttaagtcagg aaattcagga tgtgatgcgc cagcaggtgc agaaactggc 
2451 cttcgaattg caggtgcgcg gcctgatgaa cgtgcagttt gcggtgaaaa acaacgaagt ctacctgatt 
2521 gaagttaacc cgcgtgcggc gcgtaccgtt ccgttcgtct ccaaagccac cggcgtaccg ctggcaaaag 
2591 tggcggcgcg cgtgatggct ggcaaatcgc tggctgagca gggcgtaacc aaagaagtta tcccgccgta 
2661 ctactcggtg aaagaagtgg tgctgccgtt caataaattc ccgggcgttg acccgctgtt agggccagaa 
2731 atgcgctcta ccggggaagt catgggcgtg ggccgcacct tcgctgaagc gtttgccaaa gcgcagctgg 
2801 gcagcaactc caccatgaag aaacacggtc gtgcgctgct ttccgtgcgc gaaggcgata aagaacgcgt 
2871 ggtggacctg gcggcaaaac tgctgaaaca gggcttcgag ctggatgcga cccacggcac ggcgattgtg 
2941 ctgggcgaag caggtatcaa cccgcgtctg gtaaacaagg tgcatgaagg ccgtccgcac attcaggacc 
3011 gtatcaagaa tggcgaatat acctacatca tcaacaccac ctcaggccgt cgtgcgattg aagactcccg 
3081 cgtgattcgt cgcagtgcgc tgcaatataa agtgcattac gacaccaccc tgaacggcgg ctttgccacc 
3151 gcgatggcgc tgaatgccga tgcgactgaa aaagtaattt cggtgcagga aatgcacgca cagatcaaat 
3221 aaTAGCGTGT CATGGCAGAT ATTTTTCATC CGCTAATTTG ATCGAATAAC TAATACGGTT CTCTGATGAG 
3291 GACCGTTTTT TTTTGCCCAT TAAGTAAATC TT