MG1655
W3110
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Gene Report : metE
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR04 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  metE
Related strains
     Strains Search Search strains by gene name:  metE   Search strains by all related name:  metE b3829 ECK3823 JW3805 metB12

General information  (Go to Linear View:)
 Gene Name metE  
 Alternative name b3829,ECK3823,JW3805,metB12  
 Location, Length 4,011,076 - 4,013,337 (  +  ) ;   86.45 min ; 2262 (bp) ,   753 (aa) Go to Linear View
 Product 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase  
 Operon Name metE  
 Note tetrahydropteroyltriglutamate methyltransferase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009086 - methionine biosynthetic process; GO_process: GO:0006556 - S-adenosylmethionine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Methionine  
 Gene Ontology GO:0003871 ; 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity ( metE )
GO:0005515 ; protein binding ( metE )
GO:0008168 ; methyltransferase activity ( metE )
GO:0008270 ; zinc ion binding ( metE )
GO:0008652 ; amino acid biosynthetic process ( metE )
GO:0009086 ; methionine biosynthetic process ( metE )
GO:0016740 ; transferase activity ( metE )
GO:0046872 ; metal ion binding ( metE )
 PID 2367304  
 EC number
  (KEGG Pathway)   		2.1.1.14  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene metE ( EG10584 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) METE_ECOLI ( P25665 )
    -  Protein name 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase  
    -  Synonyms EC 2.1.1.14, Methionine synthase, vitamin-B12 independent isozyme, Cobalamin-independent methionine synthase  
    -  Gene name Name=metE; OrderedLocusNames=b3829;  

 Linear View (Whole Mode)
View Location
   4001.076  –  4026.076 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP metE (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, A. Thaliana Cobalamine Independent Methionine Synthase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase  
         PSI-BLAST 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    nr     (database updated : 2007.02.20 )
         BLAST 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION     

Other Cross-Reference
    COG COG0620E 
    EcoCyc metE 

MMBR References
Mol Gen Genet. 1988;211(1):78-87.
Cloning and characterization of the genes for the two homocysteine transmethylases of Escherichia coli.
Old IG, Hunter MG, Wilson DT, Knight SM, Weatherston CA, Glass RE. ( 2830470 )
J Mol Biol. 1988;200(3):427-38.
Generation of a detailed physical and genetic map of the ilv-metE-udp region of the Escherichia coli chromosome.
Aldea M, Maples VF, Kushner SR. ( 3294419 )
Sov. Genet. (Engl. Transl. Genetika) 1981;17:1127-1133.
Regulation of the activity of the uridine phosphorylase of Escherichia coli K-12. I. Mapping of mutations for the structural gene and determination of the direction of transcription.
Alkhimova, R. A., A. S. Mironov, and V. V. Sukhodolets.
Science. 1992;257(5071):771-8.
Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes.
Daniels DL, Plunkett G 3rd, Burland VD, Blattner FR. ( 1379743 )

Sequences
Amino acid
FASTA format 
0001 MTILNHTLGF PRVGLRRELK KAQESYWAGN STREELLAVG RELRARHWDQ QKQAGIDLLP VGDFAWYDHV 
0071 LTTSLLLGNV PARHQNKDGS VDIDTLFRIG RGRAPTGEPA AAAEMTKWFN TNYHYMVPEF VKGQQFKLTW 
0141 TQLLDEVDEA LALGHKVKPV LLGPVTWLWL GKVKGEQFDR LSLLNDILPV YQQVLAELAK RGIEWVQIDE 
0211 PALVLELPQA WLDAYKPAYD ALQGQVKLLL TTYFEGVTPN LDTITALPVQ GLHVDLVHGK DDVAELHKRL 
0281 PSDWLLSAGL INGRNVWRAD LTEKYAQIKD IVGKRDLWVA SSCSLLHSPI DLSVETRLDA EVKSWFAFAL 
0351 QKCHELALLR DALNSGDTAA LAEWSAPIQA RRHSTRVHNP AVEKRLAAIT AQDSQRANVY EVRAEAQRAR 
0421 FKLPAWPTTT IGSFPQTTEI RTLRLDFKKG NLDANNYRTG IAEHIKQAIV EQERLGLDVL VHGEAERNDM 
0491 VEYFGEHLDG FVFTQNGWVQ SYGSRCVKPP IVIGDISRPA PITVEWAKYA QSLTDKPVKG MLTGPVTILC 
0561 WSFPREDVSR ETIAKQIALA LRDEVADLEA AGIGIIQIDE PALREGLPLR RSDWDAYLQW GVEAFRINAA 
0631 VAKDDTQIHT HMCYCEFNDI MDSIAALDAD VITIETSRSD MELLESFEEF DYPNEIGPGV YDIHSPNVPS 
0701 VEWIEALLKK AAKRIPAERL WVNPDCGLKT RGWPETRAAL ANMVQAAQNL RRG
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             CCCGGTCACT TACTTCAGTA AGCTCCCGGG 
-070 GATGAATAAA CTTGCCGCCT TCCCTAAATT CAAAATCCAT AGGATTTACA TATAATTAGA GGAAGAAAAA 
0001 atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag 
0071 aaagttattg ggcggggaac tccacgcgtg aagaactgct ggcggtaggg cgtgaattgc gtgctcgtca 
0141 ctgggatcaa caaaagcaag cgggtatcga cctgctgccg gtgggcgatt ttgcctggta cgatcatgta 
0211 ctgaccacca gtctgctgct gggtaacgtt ccggcgcgtc atcagaacaa agatggttcg gtagatatcg 
0281 acaccctgtt ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa 
0351 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa actgacctgg 
0421 acgcagctgc tggacgaagt ggacgaggcg ctggcgctgg gccacaaggt gaaacctgtg ctgctggggc 
0491 cggttacctg gctgtggctg gggaaagtga aaggtgaaca atttgaccgc ctgagcctgc tgaacgacat 
0561 tctgccggtt tatcagcaag tgctggcaga actggcgaaa cgcggcatcg agtgggtaca gattgatgaa 
0631 cccgcgctgg tactggaact accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg 
0701 gacaggtgaa actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct 
0771 gcctgttcag ggtctgcatg ttgacctcgt acatggtaaa gatgacgttg ctgaactgca caagcgcctg 
0841 ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg gcgcgccgat cttaccgaga 
0911 aatatgcgca aattaaggac attgtcggca aacgtgattt gtgggtggca tcttcctgct cgttgctgca 
0981 cagccccatc gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta 
1051 caaaaatgcc atgaactggc actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt 
1121 ggagcgcccc gattcaggca cgtcgtcact ctacccgcgt acataatccg gcggtagaaa agcgtctggc 
1191 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt 
1261 tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac cacggaaatt cgtaccctgc 
1331 gtctggattt caaaaagggc aatctcgacg ccaacaacta ccgcacgggc attgcggaac atatcaagca 
1401 ggccattgtt gagcaggaac gtttgggact ggatgtgctg gtacatggcg aggccgagcg taatgacatg 
1471 gtggaatact ttggcgagca cctcgacgga tttgtcttta cgcaaaacgg ttgggtacag agctacggtt 
1541 cccgctgcgt gaagccaccg attgtcattg gtgacattag ccgcccggca ccgattaccg tggagtgggc 
1611 gaagtatgcg caatcgctga ccgacaaacc ggtgaaaggg atgctgacgg ggccggtgac catactctgc 
1681 tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat tgcgctggcg ctgcgtgatg 
1751 aagtggccga tctggaagcc gctggaattg gcatcatcca gattgacgaa ccggcgctgc gcgaaggttt 
1821 accgctgcgt cgtagcgact gggatgcgta tctccagtgg ggcgtagagg ccttccgtat caacgccgcc 
1891 gtggcgaaag atgacacaca aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga 
1961 ttgcggcgct ggacgcagac gtcatcacca tcgaaacctc gcgttccgac atggagttgc tggagtcgtt 
2031 tgaagagttt gattatccaa atgaaatcgg tcctggcgtc tatgacattc actcgccaaa cgtaccgagc 
2101 gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc agagcgcctg tgggtcaacc 
2171 cggactgtgg cctgaaaacg cgcggctggc cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc 
2241 gcagaacttg cgtcgggggt aaAATCCAAA CCGGGTGGTA ATACCACCCG GTCTTTTCTC ATTACAGCGA 
2311 CTTCTTCCCA CCATACTGCT TAAACCATTC CAGCATACGC TGCCAGCCAT CT