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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Deletion
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OCL79,81-3 (BK5-5 / AK4-5)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
argS
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Related strains
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Strains Search
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Search strains by gene name:
argS
Search strains by all related name:
argS b1876 ECK1877 JW1865 lov
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General information (Go to Linear View: )
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Gene Name
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argS
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Alternative name
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b1876,ECK1877,JW1865,lov
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Location, Length
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1,958,086 - 1,959,819
(
+
)
;
42.20
min
;
1734
(bp) ,
577
(aa)
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Product
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arginyl-tRNA synthetase
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Operon Name
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argS
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Note
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arginine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( argS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( argS )
GO:0004814
;
arginine-tRNA ligase activity ( argS )
GO:0005524
;
ATP binding ( argS )
GO:0005737
;
cytoplasm ( argS )
GO:0006412
;
translation ( argS )
GO:0006418
;
tRNA aminoacylation for protein translation ( argS )
GO:0006420
;
arginyl-tRNA aminoacylation ( argS )
GO:0016874
;
ligase activity ( argS )
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PID
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1788184
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EC number
(KEGG Pathway)
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6.1.1.19
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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argS
(
EG10071
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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SYR_ECOLI
(
P11875
)
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- Protein name
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Arginyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.19, Arginine--tRNA ligase, ArgRS
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- Gene name
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Name=argS; OrderedLocusNames=b1876;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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argS
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Yeast Arginyl-Trna Synthetase
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Arginyl-tRNA synthetase
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PSI-BLAST
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Arginyl-tRNA synthetase
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nr
(database updated :
2007.02.20
)
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BLAST
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arginyl-tRNA synthetase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AA_TRNA_LIGASE_I
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MMBR References
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Nucleic Acids Res. 1989;17:5725-5736
Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase.
Eriani, G., G. Dirheimer, and J. Gangloff.
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J Mol Biol. 1968;35(1):83-93.
Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. 3. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants.
Hirshfield IN, Horn PC, Hopwood DA, Maas WK, DeDeken R.
(
4939781
)
|
Microbiol. Rev. 1983;47:231-284.
Gene-protein index of Escherichia coli K-12.
Neidhardt, F. C., V. Vaughn, T. A. Phillips, and P. L. Bloch.
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EMBO J. 1992;11:1493-1501
Penicillin binding protein 2 is dispensable in Escherichia coli when ppGpp synthesis is induced.
Vinella, D., R. D'Ari, and P. Bouloc.
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| Sequences |
Amino acid
FASTA format
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0001 MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK LGMAPRQLAE QVLTHLDLNG
0071 IASKVEIAGP GFINIFLDPA FLAEHVQQAL ASDRLGVATP EKQTIVVDYS APNVAKEMHV GHLRSTIIGD
0141 AAVRTLEFLG HKVIRANHVG DWGTQFGMLI AWLEKQQQEN AGEMELADLE GFYRDAKKHY DEDEEFAERA
0211 RNYVVKLQSG DEYFREMWRK LVDITMTQNQ ITYDRLNVTL TRDDVMGESL YNPMLPGIVA DLKAKGLAVE
0281 SEGATVVFLD EFKNKEGEPM GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA
0351 IVRKAGYVPE SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER ARRLVAEKNP DMPADELEKL
0421 ANAVGIGAVK YADLSKNRTT DYIFDWDNML AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ LAAAPVIIRE
0491 DREAQLAARL LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL SAENEEVRNS RLKLAQLTAK
0561 TLKLGLDTLG IETVERM
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACGTTGCTGA CGAAAACAGC CATTTGCAGT
-070 ATACTCCCGC CCTAATTTCT TTAACTGGTG CGGGCAATTT TTGCTCGCTT CATCAATGTA AGGTATTCCG
0001 gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
0071 gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca tgatggcagt
0141 tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga ctcatctgga tcttaacggt
0211 atcgccagca aagttgagat cgccggtcca ggctttatca acattttcct tgatccggca ttcctggctg
0281 aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt
0351 tgactactct gcgccaaacg tggcgaaaga gatgcatgtc ggtcacctgc gctctaccat tattggtgac
0421 gcagcagtgc gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
0491 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa tggagctggc
0561 tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gatgaagatg aagagttcgc cgagcgcgca
0631 cgtaactacg tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca
0701 tcaccatgac gcagaaccag atcacctacg atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg
0771 cgaaagcctc tacaacccga tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa
0841 agcgaagggg cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
0911 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc gttatgaaac
0981 actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac acctgatgca ggcatgggcg
1051 atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg
1121 gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc tggatgaagc
1191 cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag ccgacgagct ggaaaaactg
1261 gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
1331 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgcat acacgcgtgt
1401 attgtccgtg ttccgtaaag cagaaattga cgaagagcaa ctggctgcag ctccggttat catccgtgaa
1471 gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag
1541 gcacgccgca tgtaatgtgt gcttacctgt acgatctggc cggtctgttc tctggcttct acgagcactg
1611 cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctaaaac tggcacaact gacggcgaag
1681 acgctgaagc tgggtctgga tacgctgggt attgagactg tagagcgtat gtaaTCGATT TTTCGTGAGA
1751 GTGAAGCCTG ATCAGGGTTA GCCGATCAGG CTTTTTTATT GCCATCTAAA TGTATTCTGA AAATGGACAT
1821 GCCATTGTTT TCTC
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