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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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FEBS Lett. 1998;439(1-2):51-4.
Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins.
Tajima T, Yokota N, Matsuyama S, Tokuda H.
(
9849875
)
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Deletion
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OCR51
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
lolA
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Related strains
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Strains Search
|
Search strains by gene name:
lolA
Search strains by all related name:
lolA b0891 ECK0882 JW0874 lplA yzzV
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General information (Go to Linear View: )
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Gene Name
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lolA
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Alternative name
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b0891,ECK0882,JW0874,lplA,yzzV
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Location, Length
|
936,595 - 937,206
(
+
)
;
20.19
min
;
612
(bp) ,
203
(aa)
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Product
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lipoprotein chaperone
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Operon Name
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lolA-rarA
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Note
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periplasmic protein effects translocation of lipoproteins from inner membrane to outer; GO_component: GO:0042597 - periplasmic space
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Function
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transport; Protein, peptide secretion
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( lplA )
GO:0003824
;
catalytic activity ( lplA )
GO:0005515
;
protein binding ( lolA )
GO:0005515
;
protein binding ( lplA )
GO:0005524
;
ATP binding ( lplA )
GO:0005737
;
cytoplasm ( lplA )
GO:0006464
;
protein modification process ( lplA )
GO:0006810
;
transport ( lolA )
GO:0008565
;
protein transporter activity ( lolA )
GO:0015031
;
protein transport ( lolA )
GO:0016740
;
transferase activity ( lplA )
GO:0016979
;
lipoate-protein ligase activity ( lplA )
GO:0018055
;
peptidyl-lysine lipoylation ( lplA )
GO:0030288
;
outer membrane-bounded periplasmic space ( lolA )
GO:0042597
;
periplasmic space ( lolA )
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PID
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145693108
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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lolA
(
EG12684
)
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Number of removed
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22 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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LOLA_ECOLI
(
P61316
;
P39178; Q8X5H8
)
|
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- Protein name
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Outer-membrane lipoprotein carrier protein precursor
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- Synonyms
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P20
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- Gene name
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Name=lolA; Synonyms=lplA; OrderedLocusNames=b0891;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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lolA
(
homologous genes of other bacterias
)
lplA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Crystal Structure Of The Lipoprotein Localization Factor, Lola
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Outer-membrane lipoprotein carrier protein precursor (P20)
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PSI-BLAST
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Outer-membrane lipoprotein carrier protein precursor (P20)
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nr
(database updated :
2007.02.20
)
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BLAST
|
outer-membrane lipoprotein carrier protein precursor [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
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EMBO J. 1997;16:6947-6955
A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli.
Matsuyama, S., N. Yokota, and H. Tokuda.
|
EMBO J. 1995;14:3365-3372
A novel periplasmic carrier protein involved in the sorting and transport of Escherichia coli lipoproteins destined for the outer membrane.
Matsuyama, S., T. Tajima, and H. Tokuda.
|
| Sequences |
Amino acid
FASTA format
|
0001 MKKIAITCAL LSSLVASSVW ADAASDLKSR LDKVSSFHAS FTQKVTDGSG AAVQEGQGDL WVKRPNLFNW
0071 HMTQPDESIL VSDGKTLWFY NPFVEQATAT WLKDATGNTP FMLIARNQSS DWQQYNIKQN GDDFVLTPKA
0141 SNGNLKQFTI NVGRDGTIHQ FSAVEQDDQR SSYQLKSQQN GAVDAAKFTF TPPQGVTVDD QRK
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GAAAATTCAG TATTTTCTTC TTTCCTCAAG
-070 CTGATTATTA GCCTGGAATA GAGAGTAGAG GGAACTCCCG ATCGGGAGTG ACGTAATTTG AGGAATAATG
0001 atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg gctgatgccg
0071 caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc ttcacacaaa aagtgactga
0141 cggtagcggc gcggcggtgc aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg
0211 catatgacac aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
0281 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa
0351 ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc
0421 agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac aatccatcag tttagcgcgg
0491 tggagcagga cgatcagcgc agcagttatc aactgaaatc ccagcaaaat ggggctgtgg atgcagcgaa
0561 atttaccttc accccgccgc aaggcgtcac ggtagatgat caacgtaagt agAGGCACCT GAGTGAGCAA
0631 TCTGTCGCTC GATTTTTCGG ATAATACTTT TCAACCTCTG GCCGCGCGTA TGCGGCCAGA AAATTTAGCA
0701 CAGTATATCG GC
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