|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCR30
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
leuB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
leuB
Search strains by all related name:
leuB b0073 ECK0075 JW5807
|
General information (Go to Linear View: )
|
|
Gene Name
|
leuB
|
|
Alternative name
|
b0073,ECK0075,JW5807
|
|
Location, Length
|
80,867 - 81,958
(
-
)
;
1.74
min
;
1092
(bp) ,
363
(aa)
|
|
Product
|
3-isopropylmalate dehydrogenase, NAD(+)-dependent
|
|
Operon Name
|
leuLABCD
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009098 - leucine biosynthetic process
|
|
Function
|
enzyme; Amino acid biosynthesis: Leucine
|
|
Gene Ontology
|
GO:0000287
;
magnesium ion binding ( leuB )
GO:0003862
;
3-isopropylmalate dehydrogenase activity ( leuB )
GO:0005737
;
cytoplasm ( leuB )
GO:0008152
;
metabolic process ( leuB )
GO:0008652
;
amino acid biosynthetic process ( leuB )
GO:0009082
;
branched chain family amino acid biosynthetic process ( leuB )
GO:0009098
;
leucine biosynthetic process ( leuB )
GO:0016491
;
oxidoreductase activity ( leuB )
GO:0016616
;
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor ( leuB )
GO:0030145
;
manganese ion binding ( leuB )
GO:0046872
;
metal ion binding ( leuB )
|
|
PID
|
87081683
|
EC number
(KEGG Pathway)
|
1.1.1.85
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
leuB
(
EG11577
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
LEU3_ECOLI
(
P30125
;
P78043
)
|
|
- Protein name
|
3-isopropylmalate dehydrogenase
|
|
- Synonyms
|
EC 1.1.1.85, Beta-IPM dehydrogenase, IMDH, 3-IPM-DH
|
|
- Gene name
|
Name=leuB; OrderedLocusNames=b0073;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
leuB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, 3-Isopropylmalate Dehydrogenase From Escherichia Coli
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
3-isopropylmalate dehydrogenase
|
|
PSI-BLAST
|
3-isopropylmalate dehydrogenase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
COG0473: Isocitrate/isopropylmalate dehydrogenase [Escherichia coli E24377A]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
IDH_IMDH
|
|
MMBR References
|
J Bacteriol. 1977;129(2):1078-90.
Isolation and characterization of lambda pleu bacteriophages.
Davis MG, Calvo JM.
(
320178
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKNYHIAVL PGDGIGPEVM TQALKVLDAV RNRFAMRITT SHYDVGGAAI DNHGQPLPPA TVEGCEQADA
0071 VLFGSVGGPK WEHLPPDQQP ERGALLPLRK HFKLFSNLRP AKLYQGLEAF CPLRADIAAN GFDILCVREL
0141 TGGIYFGQPK GREGSGQYEK AFDTEVYHRF EIERIARIAF ESARKRRHKV TSIDKANVLQ SSILWREIVN
0211 EIATEYPDVE LAHMYIDNAT MQLIKDPSQF DVLLCSNLFG DILSDECAMI TGSMGMLPSA SLNEQGFGLY
0281 EPAGGSAPDI AGKNIANPIA QILSLALLLR YSLDADDAAC AIERAINRAL EEGIRTGDLA RGAAAVSTDE
0351 MGDIIARYVA EGV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ccatggtgca cgttctgaac aatatctggc
-070 gtgccgcaga agtcgaaaaa gagttgcaac gcaaagctca acacaacgaa aacaacaagg aaaccgtgtg
0001 atgtcgaaga attaccatat tgccgtattg ccgggggacg gtattggtcc ggaagtgatg acccaggcgc
0071 tgaaagtgct ggatgccgtg cgcaaccgct ttgcgatgcg catcaccacc agccattacg atgtaggcgg
0141 cgcagccatt gataaccacg ggcaaccact gccgcctgcg acggttgaag gttgtgagca agccgatgcc
0211 gtgctgtttg gctcggtagg cggcccgaag tgggaacatt taccaccaga ccagcaacca gaacgcggcg
0281 cgctgctgcc tctgcgtaag cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct
0351 ggaagcattc tgtccgctgc gtgcagacat tgccgcaaac ggcttcgaca tcctgtgtgt gcgcgaactg
0421 accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa gcctttgata
0491 ccgaggtgta tcaccgtttt gagatcgaac gtatcgcccg catcgcgttt gaatctgctc gcaagcgtcg
0561 ccacaaagtg acgtcgatcg ataaagccaa cgtgctgcaa tcctctattt tatggcggga gatcgttaac
0631 gagatcgcca cggaataccc ggatgtcgaa ctggcgcata tgtacatcga caacgccacc atgcagctga
0701 ttaaagatcc atcacagttt gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg
0771 cgcaatgatc actggctcga tggggatgtt gccttccgcc agcctgaacg agcaaggttt tggactgtat
0841 gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa cccgattgca caaatccttt
0911 cgctggcact gctgctgcgt tacagcctgg atgccgatga tgcggcttgc gccattgaac gcgccattaa
0981 ccgcgcatta gaagaaggca ttcgcaccgg ggatttagcc cgtggcgctg ccgccgttag taccgatgaa
1051 atgggcgata tcattgcccg ctatgtagca gaaggggtgt aatcatggct aagacgttat acgaaaaatt
1121 gttcgacgct cacgttgtgt acgaagccga aaacgaaacc ccactgttat atatcgaccg ccacctggtg
1191 ca
|
