|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V.
(
8736541
)
|
|
|
Deletion
|
OCR67
(D)
,
OCR67-3
(D)
,
OCR67-5
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
narI
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
narI
Search strains by all related name:
narI b1227 chlI ECK1221 JW1218
|
General information (Go to Linear View: )
|
|
Gene Name
|
narI
|
|
Alternative name
|
b1227,chlI,ECK1221,JW1218
|
|
Location, Length
|
1,285,072 - 1,285,749
(
+
)
;
27.70
min
;
678
(bp) ,
225
(aa)
|
|
Product
|
nitrate reductase 1, gamma (cytochrome b(NR)) subunit
|
|
Operon Name
|
narGHJI
|
|
Note
|
nitrate reductase 1, cytochrome b(NR), gamma subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0017004 - cytochrome complex assembly
|
|
Function
|
enzyme; Energy metabolism, carbon: Anaerobic respiration
|
|
Gene Ontology
|
GO:0005506
;
iron ion binding ( narI )
GO:0005886
;
plasma membrane ( narI )
GO:0006118
;
electron transport ( narI )
GO:0006810
;
transport ( narI )
GO:0008940
;
nitrate reductase activity ( narI )
GO:0009325
;
nitrate reductase complex ( narI )
GO:0016020
;
membrane ( narI )
GO:0016021
;
integral to membrane ( narI )
GO:0016491
;
oxidoreductase activity ( narI )
GO:0042128
;
nitrate assimilation ( narI )
GO:0046872
;
metal ion binding ( narI )
|
|
PID
|
1787480
|
EC number
(KEGG Pathway)
|
1.7.99.4
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
narI
(
EG10640
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NARI_ECOLI
(
P11350
)
|
|
- Protein name
|
Respiratory nitrate reductase 1 gamma chain
|
|
- Synonyms
|
EC 1.7.99.4, Nitrate reductase A gamma subunit, Quinol-nitrate oxidoreductase gamma subunit, Cytochrome B-NR
|
|
- Gene name
|
Name=narI; Synonyms=chlI; OrderedLocusNames=b1227;
|
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
narI
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain C, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Respiratory nitrate reductase 1 gamma chain
|
|
PSI-BLAST
|
Respiratory nitrate reductase 1 gamma chain
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
nitrate reductase 1, gamma (cytochrome b(NR)) subunit [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Bacteriol. 1983;154(2):719-27.
Membrane cytochromes of Escherichia coli chl mutants.
Hackett NR, Bragg PD.
(
6302081
)
|
J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP.
(
1999382
)
|
J Gen Microbiol. 1980;117(1):257-62.
A mutant defective in electron transfer to nitrate in Escherichia coli K12.
Orth V, Chippaux M, Pascal MC.
(
6993626
)
|
J Bacteriol. 1984;159(1):159-66.
Construction in vitro of a cloned nar operon from Escherichia coli.
Rondeau SS, Hsu PY, DeMoss JA.
(
6330027
)
|
J Bacteriol. 1988;170(4):1721-9.
narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
Sodergren EJ, DeMoss JA.
(
2832376
)
|
J Biol Chem. 1988;263:16156-16162
Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.
Sodergren, E. J., P. Y. Hsu, and J. A. DeMoss.
|
J Bacteriol. 1982;151(2):788-99.
Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.
Stewart V, MacGregor CH.
(
7047497
)
|
Mol Gen Genet. 1981;181(4):535-40.
Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon.
Bonnefoy-Orth V, Lepelletier M, Pascal MC, Chippaux M.
(
7022129
)
|
Mol Gen Genet. 1981;182(3):477-9.
Operon fusions in the nitrate reductase operon and study of the control gene nir R in Escherichia coli.
Chippaux M, Bonnefoy-Orth V, Ratouchniak J, Pascal MC.
(
6457962
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MQFLNMFFFD IYPYIAGAVF LIGSWLRYDY GQYTWRAASS QMLDRKGMNL ASNLFHIGIL GIFVGHFFGM
0071 LTPHWMYEAW LPIEVKQKMA MFAGGASGVL CLIGGVLLLK RRLFSPRVRA TTTGADILIL SLLVIQCALG
0141 LLTIPFSAQH MDGSEMMKLV GWAQSVVTFH GGASQHLDGV AFIFRLHLVL GMTLFLLFPF SRLIHIWSVP
0211 VEYLTRKYQL VRARH
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACAAAGGCTG CGGTGATTCA GCAATCACTG
-070 CGCATCAGCG TCGCTTTGCC GGTGCCGTCG CGCCGCAATA TCTGAATATC ACCACCGGAG GACAGCACTA
0001 atgcaattcc tgaatatgtt cttctttgat atctacccgt acatagccgg ggcggtcttc ctgattggta
0071 gctggctgcg ttatgactac gggcagtaca cctggcgcgc ggcgtccagc cagatgctgg atcgcaaagg
0141 gatgaacctg gcgtcgaacc tgttccatat cgggattctg gggatttttg tcggtcactt cttcggtatg
0211 ctgacgccgc actggatgta tgaagcctgg ctgccgattg aagtgaaaca gaaaatggca atgtttgctg
0281 gtggtgccag cggcgtgctg tgtctgattg gcggcgtgct gttgctgaaa cgtcgtctgt tcagcccacg
0351 cgtgcgtgca accactaccg gagcggatat cctgatcctg tcgctgctcg ttatccagtg cgcgctgggc
0421 ctgttgacca ttccgttctc cgctcagcat atggacggta gcgagatgat gaaactggtt ggctgggcgc
0491 agtcggtggt gaccttccac ggtggcgctt ctcaacacct cgatggtgtg gcgtttatct tccgtctgca
0561 cctggtgctg gggatgacgt tattcctgct gttcccgttc tcgcgtctga tacacatctg gagcgtaccg
0631 gtggagtatc tgacacgtaa gtaccagctg gtgcgcgctc gtcactaaGC GAATTTTAGT TCACATAGAC
0701 CCTGCTTCGG CGGGGTTTTT TTATGGGCAC GGTGCGGGGT GAGTTGTCGG ATGCGCTTCG CTTATCCGAC
0771 CTACAGGG
|
