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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
|
DE(araA-leu)7697
,
OCR30
(D)
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Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
thiB
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Related strains
|
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Strains Search
|
Search strains by gene name:
thiB
Search strains by all related name:
thiB b0068 ECK0069 f327 JW0067 sfuA tbpA yabL
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General information (Go to Linear View: )
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Gene Name
|
thiB
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Alternative name
|
b0068,ECK0069,f327,JW0067,sfuA,tbpA,yabL
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Location, Length
|
74,497 - 75,480
(
-
)
;
1.61
min
;
984
(bp) ,
327
(aa)
|
|
Product
|
thiamin transporter subunit
|
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Operon Name
|
sgrR-sroA-tbpA-thiPQ
|
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Note
|
thiamin-binding periplasmic protein; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0009228 - thiamin biosynthetic process
|
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Function
|
putative transport; Transport of small molecules: Other
|
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Gene Ontology
|
GO:0005215
;
transporter activity ( thiB )
GO:0005524
;
ATP binding ( thiB )
GO:0006810
;
transport ( thiB )
GO:0030288
;
outer membrane-bounded periplasmic space ( thiB )
GO:0042597
;
periplasmic space ( thiB )
GO:0042626
;
ATPase activity, coupled to transmembrane movement of substances ( thiB )
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|
PID
|
1786255
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EC number
(KEGG Pathway)
|
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
tbpA
(
EG11574
)
|
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Number of removed
|
18 aa cleaved
|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
TBPA_ECOLI
(
P31550
;
P75637
)
|
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- Protein name
|
Thiamine-binding periplasmic protein precursor.
|
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- Synonyms
|
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- Gene name
|
Name=tbpA; Synonyms=thiB; OrderedLocusNames=b0068;
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Homology Analysis
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BLAST
|
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Bacteria
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GTOP
|
tbpA
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Ferric Binding Protein From Campylobacter Jejuni
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Thiamine-binding periplasmic protein precursor
|
|
PSI-BLAST
|
Thiamine-binding periplasmic protein precursor
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
thiamin binding protein [Escherichia coli]
|
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
|
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PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
SBP_BACTERIAL_1
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MMBR References
|
GenBank submission U09984. 1994
E. coli periplasmic thiamin binding protein: cloning, overexpression, purification, and characterization.
Hollenbach, A. D., K. A. Dickson, and M. W. Washabaugh.
|
J Bacteriol. 1993;175:982-992
Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli.
Vander Horn, P. B., A. D. Backstrom, V. J. Stewart, and T. P. Begley.
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| Sequences |
Amino acid
FASTA format
|
0001 MLKKCLPLLL LCTAPVFAKP VLTVYTYDSF AADWGPGPVV KKAFEADCNC ELKLVALEDG VSLLNRLRME
0071 GKNSKADVVL GLDNNLLDAA SKTGLFAKSG VAADAVNVPG GWNNDTFVPF DYGYFAFVYD KNKLKNPPQS
0141 LKELVESDQN WRVIYQDPRT STPGLGLLLW MQKVYGDDAP QAWQKLAKKT VTVTKGWSEA YGLFLKGESD
0211 LVLSYTTSPA YHILEEKKDN YAAANFSEGH YLQVEVAART AASKQPELAQ KFLQFMVSPA FQNAIPTGNW
0281 MYPVANVTLP AGFEKLTKPA TTLEFTPAEV AAQRQAWISE WQRAVSR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tgcgctgaga aaatacccgt cgaacctgat
-070 ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt gaggtgcaaa
0001 gtgttaaaaa aatgtctgcc cctgctgttg ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg
0071 tttataccta cgattccttc gccgccgact gggggcctgg tccggtggtt aaaaaagcct ttgaagccga
0141 ctgtaattgc gaactgaaac tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa
0211 ggcaaaaaca gtaaagccga tgtggtgctg gggctggata acaacctgtt agacgccgcc agtaaaaccg
0281 gactgtttgc caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt
0351 cgtaccgttt gattatggct acttcgcctt cgtttatgac aagaacaaac tgaaaaaccc gccacaaagc
0421 ctgaaagaac tggttgagag cgatcaaaac tggcgggtga tttatcagga tccgcgcacc agtacaccgg
0491 ggctgggtct gttgctatgg atgcaaaaag tctatggcga tgacgcccca caagcctggc agaaactggc
0561 gaagaaaacg gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat
0631 ctggtactga gttacaccac ctctccggct tatcacattc tcgaagagaa gaaagataac tacgccgccg
0701 cgaacttcag cgaaggtcac tatctgcaag tggaagtcgc cgcccgcacc gctgccagca agcagccgga
0771 gctggcgcaa aaattcctcc agtttatggt ttctccggct ttccagaatg cgatcccaac cggcaactgg
0841 atgtatccgg tggcaaacgt cacgctgcct gccggttttg aaaaattgac caaacccgca accacgttgg
0911 agttcacgcc agccgaagtg gcggcacaac gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg
0981 ttaattcccg gctggttaat tccaggtgta agcgccacca cgctggtggt agcggttgcg ctggcggcgt
1051 ttctcgccct gtggtggaac gcgccgcagg atga
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