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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCR28
(D)
|
|
Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
carA
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Related strains
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Strains Search
|
Search strains by gene name:
carA
Search strains by all related name:
carA arg arg+ura b0032 cap ECK0033 JW0030 pyrA
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General information (Go to Linear View: )
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Gene Name
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carA
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Alternative name
|
arg,arg+ura,b0032,cap,ECK0033,JW0030,pyrA
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Location, Length
|
29,651 - 30,799
(
+
)
;
0.64
min
;
1149
(bp) ,
382
(aa)
|
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Product
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carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
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Operon Name
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carAB
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Note
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GO_process: GO:0006526 - arginine biosynthetic process; GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process
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Function
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enzyme; Pyrimidine ribonucleotide biosynthesis,1.5.1.3 metabolism; building block biosynthesis; amino acids; arginine,1.5.2.2 metabolism; building block biosynthesis; nucleotide; pyrimidine biosynthesis
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Gene Ontology
|
GO:0003824
;
catalytic activity ( carA )
GO:0004086
;
carbamoyl-phosphate synthase activity ( carA )
GO:0004088
;
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity ( carA )
GO:0005515
;
protein binding ( carA )
GO:0005524
;
ATP binding ( carA )
GO:0006221
;
pyrimidine nucleotide biosynthetic process ( carA )
GO:0006526
;
arginine biosynthetic process ( carA )
GO:0006541
;
glutamine metabolic process ( carA )
GO:0006807
;
nitrogen compound metabolic process ( carA )
GO:0008652
;
amino acid biosynthetic process ( carA )
GO:0009058
;
biosynthetic process ( carA )
GO:0016874
;
ligase activity ( carA )
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PID
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1786215
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EC number
(KEGG Pathway)
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6.3.5.5
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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carA
(
EG10134
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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CARA_ECOLI
(
P00907
)
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- Protein name
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Carbamoyl-phosphate synthase small chain
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- Synonyms
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EC 6.3.5.5, Carbamoyl- phosphate synthetase glutamine chain
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- Gene name
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Name=carA; Synonyms=pyrA; OrderedLocusNames=b0032, z0037, ECs0035;
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MMBR References
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J Bacteriol. 1980;143(2):914-20.
Patterns of polarity in the Escherichia coli car AB gene cluster.
Gigot D, Crabeel M, Feller A, Charlier D, Lissens W, Glansdorff N, Pierard A.
(
6162837
)
|
Proc Natl Acad Sci U S A. 1984;81:4134-4138
DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoylphosphate.
Piette, J., H. Nyunoya, C. J. Lusty, R. Cunin, G. Weyens, M. Crabeel, D. Charlier, N. Glansdorff, and A. Pierard.
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Proc Natl Acad Sci U S A. 1984;81:4139-4143
Multiple regulatory signals in the control region of the Escherichia coli carAB operon.
Bouvier, J., J. C. Patte, and P. Stragier.
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J Bacteriol. 1980;143(2):921-5.
Use of gene cloning to determine polarity of an operon: genes carAB of Escherichia coli.
Crabeel M, Charlier D, Weyens G, Feller A, Pierard A, Glansdorff N.
(
6451616
)
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| Sequences |
Amino acid
FASTA format
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0001 MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR QIVTLTYPHI GNVGTNDADE
0071 ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA
0141 ALALEKARAF PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG AKRNILRMLV
0211 DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY AITAIQKFLE TDIPVFGICL GHQLLALASG
0281 AKTVKMKFGH HGGNHPVKDV EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF
0351 QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GTTTGCCAGA AATTCGTCGG TAAGCAGATT
-070 TGCATTGATT TACGTCATCA TTGTGAATTA ATATGCAAAT AAAGTGAGTG AATATTCTCT GGAGGGTGTT
0001 ttgattaagt cagcgctatt ggttctggaa gacggaaccc agtttcacgg tcgggccata ggggcaacag
0071 gttcggcggt tggggaagtc gttttcaata cttcaatgac cggttatcaa gaaatcctca ctgatccttc
0141 ctattctcgt caaatcgtta ctcttactta tccccatatt ggcaatgtcg gcaccaatga cgccgatgaa
0211 gaatcttctc aggtacatgc acaaggtctg gtgattcgcg acctgccgct gattgccagc aacttccgta
0281 ataccgaaga cctctcttct tacctgaaac gccataacat cgtggcgatt gccgatatcg atacccgtaa
0351 gctgacgcgt ttactgcgcg agaaaggcgc acagaatggc tgcattatcg cgggcgataa cccggatgcg
0421 gcgctggcgt tagaaaaagc ccgcgcgttc ccaggtctga atggcatgga tctggcaaaa gaagtgacca
0491 ccgcagaagc ctatagctgg acacaaggga gctggacgtt gaccggtggc ctgccagaag cgaaaaaaga
0561 agacgagctg ccgttccacg tcgtggctta tgattttggt gccaagcgca acatcctgcg gatgctggtg
0631 gatagaggct gtcgcctgac catcgttccg gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag
0701 acggcatctt cctctccaac ggtcctggcg acccggcccc gtgcgattac gccattaccg ccatccagaa
0771 attcctcgaa accgatattc cggtattcgg catctgtctc ggtcatcagc tgctggcgct ggcgagcggt
0841 gcgaagactg tcaaaatgaa atttggtcac cacggcggca accatccggt taaagatgtg gagaaaaacg
0911 tggtaatgat caccgcccag aaccacggtt ttgcggtgga cgaagcaaca ttacctgcaa acctgcgtgt
0981 cacgcataaa tccctgttcg acggtacgtt acagggcatt catcgcaccg ataaaccggc attcagcttc
1051 caggggcacc ctgaagccag ccctggtcca cacgacgccg cgccgttgtt cgaccacttt atcgagttaa
1121 ttgagcagta ccgtaaaacc gctaagtaaT CAGGAGTAAA AGAGCCATGC CAAAACGTAC AGATATAAAA
1191 AGTATCCTGA TTCTGGGTGC GGGCCCGATT GTTATCGGTC AGGCGTGTGA GTTTGACTA
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