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PEC Original Annotations
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Essentiality
|
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Class
|
non-essential
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References (PMID)
|
Microbiol Mol Biol Rev. 1998;62(3):814-984
Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Berlyn MK.
(
9729611
)
|
|
|
Deletion
|
9-1
(LD)
,
DE(gpt-lac)5
,
OCR36,37-6 (LD 9C::Ap)
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
argF
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Related strains
|
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Strains Search
|
Search strains by gene name:
argF
Search strains by all related name:
argF Arg5 argD b0273 ECK0274 JW0266
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General information (Go to Linear View: )
|
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Gene Name
|
argF
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Alternative name
|
Arg5,argD,b0273,ECK0274,JW0266
|
|
Location, Length
|
288,525 - 289,529
(
-
)
;
6.22
min
;
1005
(bp) ,
334
(aa)
|
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Product
|
ornithine carbamoyltransferase 2, chain F; CP4-6 prophage
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Operon Name
|
argF
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Note
|
ornithine carbamoyltransferase 2, chain F; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006526 - arginine biosynthetic process
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Function
|
enzyme; Amino acid biosynthesis: Arginine; Phage or Prophage Related
|
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Gene Ontology
|
GO:0003824
;
catalytic activity ( argD )
GO:0003992
;
acetylornithine transaminase activity ( argD )
GO:0004585
;
ornithine carbamoyltransferase activity ( argF )
GO:0005737
;
cytoplasm ( argD )
GO:0005737
;
cytoplasm ( argF )
GO:0006520
;
amino acid metabolic process ( argF )
GO:0006525
;
arginine metabolic process ( argD )
GO:0006526
;
arginine biosynthetic process ( argD )
GO:0006526
;
arginine biosynthetic process ( argF )
GO:0008483
;
transaminase activity ( argD )
GO:0008652
;
amino acid biosynthetic process ( argD )
GO:0008652
;
amino acid biosynthetic process ( argF )
GO:0009016
;
succinyldiaminopimelate transaminase activity ( argD )
GO:0009085
;
lysine biosynthetic process ( argD )
GO:0009348
;
ornithine carbamoyltransferase complex ( argF )
GO:0016597
;
amino acid binding ( argF )
GO:0016740
;
transferase activity ( argD )
GO:0016740
;
transferase activity ( argF )
GO:0016743
;
carboxyl- or carbamoyltransferase activity ( argF )
GO:0030170
;
pyridoxal phosphate binding ( argD )
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PID
|
1786469
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EC number
(KEGG Pathway)
|
2.1.3.3
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
argF
(
EG10067
)
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Number of removed
|
1 aa cleaved
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SWISS-PROT
(
Show details
[
3
more]
)
|
Entry name(Acc.no)
|
OTC2_ECOLI
(
P06960
;
P78308
)
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- Protein name
|
Ornithine carbamoyltransferase chain F
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- Synonyms
|
EC 2.1.3.3, OTCase-2
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- Gene name
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Name=argF; OrderedLocusNames=b0273;
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Homology Analysis
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BLAST
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Bacteria
|
|
GTOP
|
argF
(
homologous genes of other bacterias
)
argD
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain A, Ornithine Transcarbamylase From Escherichia Coli
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Ornithine carbamoyltransferase chain F
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PSI-BLAST
|
Ornithine carbamoyltransferase
|
|
nr
(database updated :
2007.02.20
)
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BLAST
|
CP4-6 prophage; ornithine carbamoyltransferase 2, chain F [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
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PROSITE
|
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PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
CARBAMOYLTRANSFERASE
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MMBR References
|
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC.
(
6307829
)
|
J Bacteriol. 1992;174(11):3836-7.
Physical map location of the argFGH operon of Escherichia coli.
Hendrickson W, Rudd KE.
(
1592837
)
|
Mol Gen Genet. 1981;181(2):222-9.
Mapping of IS1 elements flanking the argF gene region on the Escherichia coli K-12 chromosome.
Hu M, Deonier RC.
(
6268939
)
|
Mol Gen Genet. 1985;201(2):347-50.
Amplification of the ArgF region in strain HfrP4X of E. coli K-12.
Jessop AP, Clugston C.
(
3003538
)
|
Eur J Biochem. 1972;27(1):93-102.
The dual genetic control of ornithine carbamolytransferase in Escherichia coli. A case of bacterial hybrid enzymes.
Legrain C, Halleux P, Stalon V, Glansdorff N.
(
4558857
)
|
Gene. 1979;5(2):159-75.
Mapping of restriction sites in the argF gene of Escherichia coli by partial endonuclease digestion of end-labeled DNA.
Moore SK, James E.
(
376404
)
|
Gene. 1981;16(1-3):119-32.
Nucleotide sequence of the argF regulatory region of Escherichia coli K-12.
Moore SK, Garvin RT, James E.
(
6282686
)
|
EMBO J. 1982;1(7):853-7.
Homologous control sites and DNA transcription starts in the related argF and argI genes of Escherichia coli K12.
Piette J, Cunin R, Van Vliet F, Charlier D, Crabeel M, Ota Y, Glansdorff N.
(
6329710
)
|
Nucleic Acids Res. 1984;12:6277-6289
Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferase-complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB.
Van Vliet, F., R. Cunin, A. Jacobs, J. Piette, D. Gigot, M. Lauwereys, A. Pierard, and N. Glansdorff.
|
Mol Gen Genet. 1981;181(2):230-40.
Characterization of P1argF derivatives from Escherichia coli K12 transduction. I. IS1 elements flank the argF gene segment.
York MK, Stodolsky M.
(
6268940
)
|
Gene. 1979;5(3):207-31.
Cloning and endonuclease restriction analysis of argF and of the control region of the argECBH bipolar operon in Escherichia coli.
Crabeel M, Charlier D, Cunin R, Glansdorff N.
(
381103
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSDLYKKHFL KLLDFTPAQF TSLLTLAAQL KADKKNGKEV QKLTGKNIAL IFEKDSTRTR CSFEVAAFDQ
0071 GARVTYLGPS GSQIGHKESI KDTARVLGRM YDGIQYRGHG QEVVETLAQY AGVPVWNGLT NEFHPTQLLA
0141 DLMTMQEHLP GKAFNEMTLV YAGDARNNMG NSMLEAAALT GLDLRLLAPK ACWPEESLVA ECSALAEKHG
0211 GKITLTEDVA AGVKGADFIY TDVWVSMGEA KEKWAERIAL LRGYQVNAQM MALTDNPNVK FLHCLPAFHD
0281 DQTTLGKQMA KEFDLHGGME VTDEVFESAA SIVFDQAENR MHTIKAVMMA TLGE
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cctttgtaag aaagaattgt gaaatggggt
-070 tgcaaatgaa taattacaca tataaagtga attttaattc aataagtggc gttcgccatg cgaggataaa
0001 atgtccgatt tatacaaaaa acactttctg aaactgctcg actttacccc tgcacagttc acttctctgc
0071 tgacccttgc cgcacagctc aaagccgata aaaaaaatgg caaggaagta cagaagctta ccggtaaaaa
0141 catcgcgctc atcttcgaaa aagactcgac tcgtacccgt tgctctttcg aagttgccgc atttgaccag
0211 ggcgcgcgcg ttacctattt agggccgagc ggcagccaga ttgggcataa agagtcaatt aaggacaccg
0281 cgcgggttct cgggcggatg tatgacggca ttcagtatcg cggtcacggc caggaagtgg tcgaaacgct
0351 ggcgcagtat gcgggcgtgc cggtgtggaa cgggctgacc aacgagttcc acccgaccca gctgctggcg
0421 gacctgatga ccatgcagga gcacctgccg ggcaaggcgt ttaacgagat gacgctggtc tacgcgggcg
0491 atgcgcgcaa caacatgggc aactcgatgc tggaagcggc ggcgctgacc gggctggatc tgcgcctgtt
0561 ggccccgaaa gcctgctggc cggaagagag cctggtggcg gagtgcagcg cgctggcgga gaagcacggc
0631 gggaaaatta ctctgacgga agacgtggcg gcaggcgtta agggcgcgga ctttatctat accgacgtgt
0701 gggtgtcgat gggcgaggcc aaagagaagt gggcagagcg gattgcgctg ctgcgcgggt atcaggtgaa
0771 cgcgcagatg atggcgctga ccgacaaccc gaacgtgaag ttcctgcact gtctgccggc gttccatgac
0841 gaccagacta cgctcggcaa gcagatggcg aaggagttcg atctgcacgg cgggatggag gtgacggacg
0911 aggtgtttga gtcggcggcg agcatcgtgt tcgaccaggc ggaaaaccgg atgcatacga ttaaggcggt
0981 gatgatggca acgcttgggg agtgattggg tccgctgcgc gttggtgccc tcaccccggc cctctcccac
1051 agggagaggg agaacaccgg ctccatttca ttgatttttc atcccgaaaa aggta
|
