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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCL04
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
recF
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Related strains
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Strains Search
|
Search strains by gene name:
recF
Search strains by all related name:
recF b3700 ECK3692 f357 JW3677 uvrF
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General information (Go to Linear View: )
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Gene Name
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recF
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Alternative name
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b3700,ECK3692,f357,JW3677,uvrF
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Location, Length
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3,878,171 - 3,879,244
(
-
)
;
83.59
min
;
1074
(bp) ,
357
(aa)
|
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Product
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gap repair protein
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Operon Name
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dnaAN-recF
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Note
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ssDNA and dsDNA binding, ATP binding; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006281 - DNA repair; GO_process: GO:0006310 - DNA recombination
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Function
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regulator; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( recF )
GO:0003677
;
DNA binding ( recF )
GO:0003697
;
single-stranded DNA binding ( recF )
GO:0005515
;
protein binding ( recF )
GO:0005524
;
ATP binding ( recF )
GO:0005694
;
chromosome ( recF )
GO:0005737
;
cytoplasm ( recF )
GO:0006259
;
DNA metabolic process ( recF )
GO:0006260
;
DNA replication ( recF )
GO:0006281
;
DNA repair ( recF )
GO:0006974
;
response to DNA damage stimulus ( recF )
GO:0009432
;
SOS response ( recF )
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PID
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1790135
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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recF
(
EG10828
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
|
RECF_ECOLI
(
P03016
)
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- Protein name
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DNA replication and repair protein recF.
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- Synonyms
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- Gene name
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OrderedLocusNames=b3700, c4622, z5191, ECs4635;
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MMBR References
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Nucleic Acids Res. 1987;15:771-784
DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.
Adachi, T., M. Mizuuchi, A. Robinson, E. Appella, M. H. O'Dea, M. Gellert, and K. Mizuuchi.
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Nucleic Acids Res. 1984;12:6389-6395
DNA sequence and transcription of the region upstream of the E. coli gyrB gene.
Adachi, T., R. Mizuuchi, R. Menzel, and M. Gellert.
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Gene. 1986;43(3):183-96.
Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences.
Armengod ME, Lambies E.
(
3527871
)
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J Bacteriol. 1990;172(11):6291-9.
Purification and preliminary characterization of the Escherichia coli K-12 recF protein.
Griffin TJ 4th, Kolodner RD.
(
2228960
)
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J Bacteriol. 1994;176:7113-7114
The RecF pathway of homologous recombination can mediate the initiation of DNA damage-inducible replication of the Escherichia coli chromosome.
Asai, T., and T. Kogoma.
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Proc Natl Acad Sci U S A. 1988;85:6592-6596
Properties of a mutant recA-encoded protein reveal a possible role for Escherichia coli recF-encoded protein in genetic recombination.
Madiraju, M. V., A. Templin, and A. J. Clark.
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Plasmid. 1983;10(2):101-10.
Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome.
Ream LW, Clark AJ.
(
6356186
)
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Mol Gen Genet. 1980;180(1):115-21.
Genetic and physical mapping of recF in Escherichia coli K-12.
Ream LW, Margossian L, Clark AJ, Hansen FG, von Meyenburg K.
(
6255290
)
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Proc Natl Acad Sci U S A. 1984;81:4622-4626
Molecular analysis of the recF gene of Escherichia coli.
Blanar, M. A., S. J. Sandler, M.-E. Armengod, L. W. Ream, and A. J. Clark.
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Mol Microbiol. 1996;19(4):871-80.
Overlapping functions for recF and priA in cell viability and UV-inducible SOS expression are distinguished by dnaC809 in Escherichia coli K-12.
Sandler SJ.
(
8820655
)
|
J Bacteriol. 1994;176:4011-4016
Mutational analysis of sequences in the recF gene of Escherichia coli K-12 that affect expression.
Sandler, S. J., and A. J. Clark.
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Nucleic Acids Res. 1992;20:839-845
Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop.
Sandler, S. J., B. Chackerian, J. T. Li, and A. J. Clark.
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ICN-UCLA Symp. Mol. Cell. Biol. 1980;19:137-159.
The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations.
von Meyenburg, K., F. G. Hansen, E. Riise, H. E. N. Bergmans, M. Meijer, and W. Messer.
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Mutat Res. 1997;381(1):111-5.
Involvement of recA and recF in the induced precise excision of Tn10 in Escherichia coli.
Chan A, Nagel R.
(
9403037
)
|
Biochimie. 1991;73(4):523-32.
rec genes and homologous recombination proteins in Escherichia coli.
Clark AJ.
(
1911953
)
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| Sequences |
Amino acid
FASTA format
|
0001 MSLTRLLIRD FRNIETADLA LSPGFNFLVG ANGSGKTSVL EAIYTLGHGR AFRSLQIGRV IRHEQEAFVL
0071 HGRLQGEERE TAIGLTKDKQ GDSKVRIDGT DGHKVAELAH LMPMQLITPE GFTLLNGGPK YRRAFLDWGC
0141 FHNEPGFFTA WSNLKRLLKQ RNAALRQVTR YEQLRPWDKE LIPLAEQIST WRAEYSAGIA ADMADTCKQF
0211 LPEFSLTFSF QRGWEKETEY AEVLERNFER DRQLTYTAHG PHKADLRIRA DGAPVEDTLS RGQLKLLMCA
0281 LRLAQGEFLT RESGRRCLYL IDDFASELDD ERRGLLASRL KATQSQVFVS AISAEHVIDM SDENSKMFTV
0351 EKGKITD
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aaaacgtccg catgatgctg accgattcgg
-070 tttccagcgt gcagattgaa gatgcggcca gccagagcgc ggcttatgtt gtcatgccaa tgagactgta
0001 atgtccctca cccgcttgtt gatccgcgat ttccgcaaca ttgaaaccgc ggatctcgcc ttatctcccg
0071 gctttaactt tctggtaggt gccaacggca gtggcaaaac cagcgtgctg gaagccatct atacgctcgg
0141 ccatggtcgg gcgtttcgca gtttgcagat tggtcgcgtc attcgccatg agcaggaggc gtttgttctc
0211 cacgggcgat tacagggcga agagcgcgag acagcgattg gcttaaccaa agacaaacag ggcgacagca
0281 aagtccgcat cgacggtaca gacgggcata aggtcgcgga actggcgcac ctgatgccaa tgcagttgat
0351 aacgccagaa gggtttactt tactcaacgg cggccccaaa tacagaagag cattcctcga ctggggatgc
0421 tttcacaacg aacccggatt tttcaccgcc tggagcaatc tcaagcgatt gctcaagcag cgcaatgcgg
0491 cgctgcgcca ggtgacacgt tacgaacagc tacgcccgtg ggataaagag ctgatcccgc tggcggagca
0561 aatcagcacc tggcgcgcgg agtatagcgc cggtatcgcg gccgatatgg ctgatacctg taagcaattt
0631 ctccctgagt tttctctgac tttctctttc cagcgcggct gggagaaaga gacagaatat gctgaggtgc
0701 tggaacgtaa ttttgaacgc gatcgccagc taacctacac cgcgcacggc ccgcacaaag cggacttacg
0771 cattcgcgcc gacggtgcgc cggtggaaga taccttatcg cgtgggcagc ttaagctgtt gatgtgcgcc
0841 ttacgtctgg cgcaaggaga gttcctcacc cgtgaaagcg ggcggcggtg tctctacctg atagatgatt
0911 ttgcctctga gcttgatgat gagcgtcgcg ggctgcttgc cagccgctta aaagcgacgc aatcacaggt
0981 ctttgtcagc gcgatcagtg ctgaacacgt tatagacatg tcggacgaaa attcgaagat gtttaccgtg
1051 gaaaagggta aaataacgga ttaacccaag tataaatgag cgagaaacgt tgatgtcgaa ttcttatgac
1121 tcctccagta tcaaagtcct gaaagggctg gatgcggtgc gtaagcgccc gggt
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