|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V.
(
8736541
)
|
|
|
Deletion
|
OCR67
(D)
,
OCR67-3
(D)
,
OCR67-5
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
narJ
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
narJ
Search strains by all related name:
narJ b1226 ECK1220 JW1217
|
General information (Go to Linear View: )
|
|
Gene Name
|
narJ
|
|
Alternative name
|
b1226,ECK1220,JW1217
|
|
Location, Length
|
1,284,362 - 1,285,072
(
+
)
;
27.68
min
;
711
(bp) ,
236
(aa)
|
|
Product
|
molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1
|
|
Operon Name
|
narGHJI
|
|
Note
|
nitrate reductase 1, delta subunit, assembly function; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0006457 - protein folding
|
|
Function
|
enzyme; Energy metabolism, carbon: Anaerobic respiration
|
|
Gene Ontology
|
GO:0005515
;
protein binding ( narJ )
GO:0006118
;
electron transport ( narJ )
GO:0008940
;
nitrate reductase activity ( narJ )
GO:0009325
;
nitrate reductase complex ( narJ )
GO:0016491
;
oxidoreductase activity ( narJ )
GO:0042128
;
nitrate assimilation ( narJ )
|
|
PID
|
1787479
|
EC number
(KEGG Pathway)
|
1.7.99.4
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
NARJ_ECOLI
(
P11351
)
|
|
- Protein name
|
Respiratory nitrate reductase 1 delta chain
|
|
- Synonyms
|
EC 1.7.99.4
|
|
- Gene name
|
Name=narJ; OrderedLocusNames=b1226, z2003, ECs1731;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
narJ
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Respiratory nitrate reductase 1 delta chain
|
|
PSI-BLAST
|
Respiratory nitrate reductase 1 delta chain
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
nitrate reductase 1 delta subunit [Escherichia coli O157:H7 str. Sakai]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Bacteriol. 1992;174:867-872
The narJ gene product is required for biogenesis of respiratory nitrate reductase in Escherichia coli.
Dubourdieu, M., and J. A. DeMoss.
|
J Bacteriol. 1991;173(5):1574-5.
Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome.
Leonardo MR, Clark DP.
(
1999382
)
|
J Biol Chem. 1997;272:24266-24271
Characterization of NarJ, a system-specific chaperone required for nitrate reductase biogenesis in Escherichia coli.
Liu, X., and J. A. DeMoss.
|
Mol Microbiol. 1996;20(4):875-84.
Involvement of the narJ and mob gene products in distinct steps in the biosynthesis of the molybdoenzyme nitrate reductase in Escherichia coli.
Palmer T, Santini CL, Iobbi-Nivol C, Eaves DJ, Boxer DH, Giordano G.
(
8793883
)
|
Mol Gen Genet. 1989;218(2):249-56.
Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.
Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V.
(
2674654
)
|
J Bacteriol. 1988;170(4):1721-9.
narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.
Sodergren EJ, DeMoss JA.
(
2832376
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MIELVIVSRL LEYPDAALWQ HQQEMFEAIA ASKNLPKEDA HALGIFLRDL TTMDPLDAQA QYSELFDRGR
0071 ATSLLLFEHV HGESRDRGQA MVDLLAQYEQ HGLQLNSREL PDHLPLYLEY LAQLPQSEAV EGLKDIAPIL
0141 ALLSARLQQR ESRYAVLFDL LLKLANTAID SDKVAEKIAD EARDDTPQAL DAVWEEEQVK FFADKGCGDS
0211 AITAHQRRFA GAVAPQYLNI TTGGQH
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCTTTGGTGA TGGCTGCCAC GGTTCAGATA
-070 CCAAATTCAA TCTGTTCAAC AGCCGTCGTA TCGATGCCAT CGATGTGACC AGCAAAACGG AGCCGCATCC
0001 atgatcgaac tcgtgattgt atcgcgtctc cttgaatatc cggatgctgc cttatggcag catcaacaag
0071 agatgtttga ggcgattgcc gcgtcgaaaa atctgccaaa agaggatgcc catgcgctgg gcattttcct
0141 gcgcgattta acgacgatgg atccgctcga tgcccaggcg cagtacagcg aactgttcga ccgtggccgc
0211 gccacgtcac tgttgctgtt tgaacatgtg cacggcgaat cccgcgaccg cggccaggcg atggtggacc
0281 tgctggcgca gtacgagcag cacggcttgc agttaaacag ccgcgaattg ccggaccatc tgccgctgta
0351 tctggagtac ctggcgcagc tgccgcaaag cgaagccgtg gaaggtttga aagatatcgc gccgattctg
0421 gcattgctga gcgcgcgtct gcaacagcgt gaaagccgtt atgccgtgct gtttgatctg ctgctgaaac
0491 tggcgaatac cgctatcgac agcgacaaag tggcggaaaa aattgccgac gaagcgcgcg atgatacgcc
0561 gcaggcgctg gatgctgtct gggaagaaga gcaggttaaa ttctttgctg acaaaggctg cggtgattca
0631 gcaatcactg cgcatcagcg tcgctttgcc ggtgccgtcg cgccgcaata tctgaatatc accaccggag
0701 gacagcacta aTGCAATTCC TGAATATGTT CTTCTTTGAT ATCTACCCGT ACATAGCCGG GGCGGTCTTC
0771 CTGATTGGTA GCTGGCTGCG TTATGACTAC GGGCAGTACA C
|
