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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Mol Gen Genet. 1983;189(2):215-21.
Mutants of Escherichia coli defective in penicillin-insensitive murein DD-endopeptidase.
Iida K, Hirota Y, Schwarz U.
(
6343788
)
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Deletion
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OCL29,30-6 (AK4-11 / BK5-11)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
dacB
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Related strains
|
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Strains Search
|
Search strains by gene name:
dacB
Search strains by all related name:
dacB b3182 ECK3171 JW3149 o477
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General information (Go to Linear View: )
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Gene Name
|
dacB
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Alternative name
|
b3182,ECK3171,JW3149,o477
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Location, Length
|
3,326,985 - 3,328,418
(
+
)
;
71.71
min
;
1434
(bp) ,
477
(aa)
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Product
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D-alanyl-D-alanine carboxypeptidase
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Operon Name
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dacB
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Note
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D-alanyl-D-alanine carboxypeptidase, fraction B; penicillin-binding protein 4; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0042493 - response to drug
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Function
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enzyme; Murein sacculus, peptidoglycan
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Gene Ontology
|
GO:0004185
;
serine carboxypeptidase activity ( dacB )
GO:0005515
;
protein binding ( dacB )
GO:0006508
;
proteolysis ( dacB )
GO:0007047
;
cell wall organization and biogenesis ( dacB )
GO:0007049
;
cell cycle ( dacB )
GO:0008360
;
regulation of cell shape ( dacB )
GO:0009002
;
serine-type D-Ala-D-Ala carboxypeptidase activity ( dacB )
GO:0009252
;
peptidoglycan biosynthetic process ( dacB )
GO:0016787
;
hydrolase activity ( dacB )
GO:0042597
;
periplasmic space ( dacB )
GO:0046677
;
response to antibiotic ( dacB )
GO:0051301
;
cell division ( dacB )
|
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PID
|
1789573
|
EC number
(KEGG Pathway)
|
3.4.16.4
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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dacB
(
EG10202
)
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Number of removed
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20 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
PBP4_ECOLI
(
P24228
)
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- Protein name
|
Penicillin-binding protein 4 precursor
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- Synonyms
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PBP-4
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- Gene name
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Name=dacB; OrderedLocusNames=b3182;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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dacB
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Crystal Structure Of Penicillin Binding Protein 4 (Dacb) From Escherichia Coli
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Penicillin-binding protein 4 precursor
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PSI-BLAST
|
Penicillin-binding protein 4 precursor
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nr
(database updated :
2007.02.20
)
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BLAST
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D-alanyl-D-alanine carboxypeptidase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
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MMBR References
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Mol Microbiol. 1991;5(3):675-84.
Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition.
Korat B, Mottl H, Keck W.
(
2046551
)
|
Proc Natl Acad Sci U S A. 1977;74:2976-2979
Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity.
Matsuhashi, M., Y. Takagaki, I. N. Maruyama, S. Tamaki, Y. Nishimura, H. Suzuki, U. Ogino, and Y. Hirota.
|
J Bacteriol. 1992;174:3261-3269
Deletion of an additional domain located between SXXK and SXN active-site fingerprints in penicillin-binding protein 4 from Escherichia coli.
Mottl, H., P. Nieland, G. De Kort, J. J. Wierenga, and W. Keck.
|
FEMS Microbiol Lett. 1991;62(2-3):213-20.
Penicillin-binding protein 4 of Escherichia coli shows a novel type of primary structure among penicillin-interacting proteins.
Mottl H, Terpstra P, Keck W.
(
2040429
)
|
J Bacteriol. 1994;176:7767-7769
Transcriptional control of dacB, which encodes a major sporulation-specific penicillin-binding protein.
Simpson, E. B., T. W. Hancock, and C. E. Buchanan.
|
Plasmid. 1981;6(1):86-98.
Synthetic ColE1 Plasmids carrying genes for penicillin-binding proteins in Escherichia coli.
Takeda Y, Nishimura A, Nishimura Y, Yamada M, Yasuda S, Suzuki H, Hirota Y.
(
7025060
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MRFSRFIIGL TSCIAFSVQA ANVDEYITQL PAGANLALMV QKVGASAPAI DYHSQQMALP ASTQKVITAL
0071 AALIQLGPDF RFTTTLETKG NVENGVLKGD LVARFGADPT LKRQDIRNMV ATLKKSGVNQ IDGNVLIDTS
0141 IFASHDKAPG WPWNDMTQCF SAPPAAAIVD RNCFSVSLYS APKPGDMAFI RVASYYPVTM FSQVRTLPRG
0211 SAEAQYCELD VVPGDLNRFT LTGCLPQRSE PLPLAFAVQD GASYAGAILK DELKQAGITW SGTLLRQTQV
0281 NEPGTVVASK QSAPLHDLLK IMLKKSDNMI ADTVFRMIGH ARFNVPGTWR AGSDAVRQIL RQQAGVDIGN
0351 TIIADGSGLS RHNLIAPATM MQVLQYIAQH DNELNFISML PLAGYDGSLQ YRAGLHQAGV DGKVSAKTGS
0421 LQGVYNLAGF ITTASGQRMA FVQYLSGYAV EPADQRNRRI PLVRFESRLY KDIYQNN
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ATAGTCATTT TACCCTGAAG TTCCCGAAGG
-070 GTCATCGTTT ACTTTATAGG GCGTTGCGCC GTAGTATGAC GGCTCGATTC CAGGTTGTTA GCGCGAGATT
0001 atgcgatttt ccagatttat catcggattg accagctgta tagcgttcag tgttcaggcc gcaaatgttg
0071 atgagtacat tactcaactc cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc
0141 ccccgctatt gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
0211 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc aatgtggaaa
0281 acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg ttaaaacgtc aggatattcg
0351 caatatggtc gcgactttga aaaaatctgg cgtcaaccaa atcgatggca atgtgttgat agatacctcc
0421 attttcgcca gccacgataa agcccccggc tggccatgga atgacatgac acaatgcttt agcgctccgc
0491 ctgccgccgc catagttgac cgcaactgtt tctccgtctc gctctacagt gccccaaagc ctggtgatat
0561 ggcttttata cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
0631 tctgccgaag cgcaatactg cgaactggat gtggtgccag gcgacctgaa ccgctttacg ctgacgggat
0701 gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat ggagccagct atgccggtgc
0771 aattctgaaa gatgagttaa aacaggcggg tatcacctgg agcggaacac tgctgcgcca gactcaggtt
0841 aacgaacctg gaacggtagt tgccagtaaa cagtcggccc cgctgcacga tctgcttaag attatgctga
0911 aaaagtcgga caacatgatc gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtgcctgg
0981 aacatggcgg gccgggtcgg acgccgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
1051 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg atgcaggtgc
1121 tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg ccgctggcgg gctatgacgg
1191 ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg gatggaaaag tctcagcgaa aaccggttcg
1261 ttgcaggggg tatataacct ggcgggattc attaccacag cgagcgggca acgaatggcg tttgtgcaat
1331 atctttctgg ctatgcagta gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag
1401 ccgtttgtat aaagatattt atcagaacaa ttagTCAAAA AGAAACCCCG GCACATGGCC GGGGCTTCAG
1471 ATTATTGACA AAGTGCGCTT TGTTTATGCC GGATGCGGCG TAAACGCCTT ATCCGGCCTA CAAA
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