BLAST Help
Available search programs
-------------------------
blastn - Comparing a nucleotide query sequence
against a nucleotide sequence database.
blastp - Comparing an amino acid query sequence
against a protein sequence database.
blastx - Comparing a nucleotide query sequence translated
in all reading frames aginst a protein sequence database.
tblastn - Comparing a protein query sequence aginst a nucleocide sequence
database dynamically translated in all reading frames.
tblastx - Comparing the six-frame translations of a nucleotide query
sequence against the six-frame translations of a nucleotide
sequence database.
Please note that tblastx program cannot be used with the DNA,
DDBJ, EMBL, GENBANK, JAPIO and CODON databases.
Parameters
---------------------
descriptions
Specify the number of one-line descriptions. [integer]
Default value: 100
alignments
Specify the number of alignments to show. [integer]
Default value: 100
expect
Specify the expected number of homologous sequences
in the database. If you need to get more sequences
with lower homology score, increase the "expect" value.
If you need only sequences with very high homology scores,
decrease the value. [Real]
Default value: 10.0
match (blastn only)
Specify the reward for a nucleotide match. [integer]
Default value: 1
mismatch (blastn only)
Specify the penalty for a nucleotide mismatch. [integer]
Default value: -3
view
Specify the alignment view options.
View parameter table:
0 = pairwise
1 = master-slave showing identities
2 = master-slave no identities
3 = flat master-slave, show identities
4 = flat master-slave, no identities
5 = master-slave no identities and blunt ends
6 = flat master-slave, no identities and blunt ends
Default value: 0
filter
The server filters your query sequence for low compositional complexity
regions by default. Low complexity regions commonly give spuriously high
scores that reflect compositional bias rather than significant position-by- position
alignment. Filtering can elminate these potentially confounding matches
(e.g., hits against proline-rich regions or poly-A tails) from the blast reports,
leaving regions whose blast statistics reflect the specificity of their pairwise
alignment. Queries searched with the blastn program are filtered with DUST. Other
programs use SEG.
Low complexity sequence found by a filter program is substituted using the
letter "N" in nucleotide sequence (e.g., "NNNNNNNNNNNNN") and the letter "X"
in protein sequences (e.g., "XXXXXXXXX").
gap_open
Specify the cost to open a gap
(zero invokes default behavior). [Integer]
Default value: blastn = 5, others = See "gap_extend"
gap_extend
Specify the cost to extend a gap
(zero invokes default behavior). [Integer]
Default value: blastn = 2, others = See the following
Sets of gap_open and gap_extend values (except blastn):
gap_open gap_extend
---------------------
11 1(default)
10 1
9 2
8 2
dropoff
Specify X dropoff value for gapped alignment (in bits)
(zero invokes default behavior). [integer]
Default value: 0
gi
Specify GI's in deflines. [T/F]
Default value: F
extend_hit
Specify the threshold for extending hits. [integer]
Default value: 0
gap_align
Perform gapped alignment [T/F]
Default value: T
query_code (blastx only)
Specify Query Genetic code to be used in blastx translation
of the query.
Query genetic code table:
1 = Standard
2 = Vertebrate Mitochondrial
3 = Yeast Mitochondrial
4 = Mold Mitochondrial
5 = Invertebrate Mitochondrial
6 = Ciliate Macronuclear
9 = Echinodermate Mitochondrial
10 = Euplotid Nuclear
11 = Bacterial
12 = Alternative Yeast Nuclear
13 = Ascidian Mitochondrial
14 = Flatworm Mitochondrial
15 = Blepharisma Macronuclear
Default value: 1